Original title: Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Authors: Farka, Z. ; Hlaváček, Antonín ; Mickert, M. J. ; Skládal, P. ; Gorris, H. H.
Document type: Papers
Conference/Event: NANOCON 2017. International Conference on Nanomaterials - Research and Application /9./, Brno (CZ), 20171018
Year: 2018
Language: eng
Abstract: Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Keywords: antibody; bioconjugation; diclofenac; immunoassay; photon-upconversion nanoparticle
Project no.: GBP206/12/G014 (CEP)
Funding provider: GA ČR
Host item entry: NANOCON 2017: Conference Proceedings, ISBN 978-80-87294-81-9

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0287392

Permalink: http://www.nusl.cz/ntk/nusl-387484


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