Original title: Study of mechanical properties and dynamics of biomembranes and single molecules with the use of laser beam - an overview
Authors: Jonáš, Alexandr ; Zemánek, Pavel
Document type: Papers
Conference/Event: Biophysics of the Genome and Its Interactions, Hlohovec (CZ), 2001-10-15 / 2001-10-17
Year: 2001
Language: eng
Abstract: A micron-sized dielectric particle confined in a laser trap can be employed as a probe for the measurement of forces in the range from piconewtons to hundreds of piconewtons. Thus, if a biological system of interest (e.g. DNA molecule, single myosin or kinesin molecule, cell membrane) is attached to such a probe, its mechanical and dynamical properties (elasticity, viscosty, forces associated with movement) can be studied during its interaction with the environment with unprecedented resolution. This article introduces the basic principles of the laser trapping and force measurement and illustrates on several examples the great potential of the light-based force transducer for exploiting non-invasively the dynamics of basic biological systems.
Keywords: cell; force measurement; laser trap
Project no.: CEZ:AV0Z2065902 (CEP), GA202/99/0959 (CEP), IAA1065203 (CEP), GA101/00/0974 (CEP)
Funding provider: GA ČR, GA AV ČR, GA ČR
Host item entry: Proceedings of the conference at Hlohovec, Czech Republic

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0101201

Permalink: http://www.nusl.cz/ntk/nusl-29570


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Research > Institutes ASCR > Institute of Scientific Instruments
Conference materials > Papers