Original title: Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Authors: Jelínková, Petra ; Tichá, M. ; Jonáková, Věra
Document type: Proceedings
Conference/Event: Biologically Active Peptides /8./, Praha (CZ), 2003-04-23 / 2003-04-25
Year: 2003
Language: eng
Series: volume 6
Abstract: Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers.
Keywords: proteinase inhibitors, aggregated forms, boar seminal plasma
Project no.: GA303/02/0433 (CEP), GP303/02/P069 (CEP), NJ7463 (CEP), CEZ:MSM 113100001
Funding provider: GA ČR, GA ČR, GA MZd

Institution: Institute of Molecular Genetics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0087342

Permalink: http://www.nusl.cz/ntk/nusl-28143


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