Název: Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Autoři: Jelínková, Petra ; Tichá, M. ; Jonáková, Věra
Typ dokumentu: Sborníky
Konference/Akce: Biologically Active Peptides /8./, Praha (CZ), 2003-04-23 / 2003-04-25
Rok: 2003
Jazyk: eng
Edice: volume 6
Abstrakt: Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers.
Klíčová slova: proteinase inhibitors, aggregated forms, boar seminal plasma
Číslo projektu: GA303/02/0433 (CEP), GP303/02/P069 (CEP), NJ7463 (CEP), CEZ:MSM 113100001
Poskytovatel projektu: GA ČR, GA ČR, GA MZd

Instituce: Ústav molekulární genetiky AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0087342

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-28143


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav molekulární genetiky
Konferenční materiály > Sborníky