Název:
Sperm protein profiles of different mammalian species
Autoři:
Pohlová, Alžběta ; Zigo, Michal ; Jonáková, Věra ; Postlerová, Pavla Typ dokumentu: Příspěvky z konference Konference/Akce: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Rok:
2015
Jazyk:
eng
Abstrakt: Proteins are a substantial equipment of the spermatic cell; therefore, the characterization of sperm proteins is crucial for explanation of molecular mechanisms in the reproduction process. We isolated sperm proteins from different mammalian species - pig, bull, human, mouse, dog and cat. Extracted proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal or ejaculated sperm were compared. Additionally, we tested cross-reactivity of antibodies prepared to sperm boar proteins on spermatozoa of other mammalian species using immunofluorescent technique. Our future plan is to compare the protein profiles of sperm during their functional development (epididymal, ejaculated, capacitated) in various mammalian species and identify species-specific sperm proteins with zona pellucida binding activity.
Klíčová slova:
monoclonal antibody; SDS-electrophoresis; sperm proteins; zona pellucida binding activity Číslo projektu: GA P503/12/1834, GA 14-05547S, ED1.1.00/02.0109 Poskytovatel projektu: GA ČR, GA ČR, GA MŠk Zdrojový dokument: Book of abstracts of XXIst Symposium of biology and immunology of reproduction
Instituce: Biotechnologický ústav AV ČR
(web)
Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0264415