Original title: Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm
Authors: Margaryan, Hasmik ; Dorosh, Andriy ; Čapková, Jana ; Postlerová, Pavla ; Philimonenko, Anatoly ; Hozák, Pavel ; Pěknicová, Jana
Document type: Papers
Conference/Event: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Year: 2015
Language: eng
Abstract: Sperm proteins are important for the structure and function of these specific, highly differentiated cells. Certain of these proteins play a role in sperm-egg recognition during primary or secondary binding at zona pellucida glycoprotein matrix. The aim of this study was to characterize the acrosomal sperm protein recognized by a monoclonal antibody (MoAb) Hs-8, prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperm, and to test the possible role of this protein in gamete interaction. MoAb Hs-8 specifically labelled a 45 kDa protein from the sperm extract in the immunoblotting test. Sequence analysis identified this Hs-8 protein as GAPDHS. In order to perform a control tests, a commercial mouse anti-GAPDHS MoAb was applied. Both antibodies showed similar staining patterns using immunofluorescence labelling, transmission electron microscopy and immunoblot analysis. Moreover, both Hs-8 and commercial anti-GAPDHS antibodies blocked the secondary sperm-zona pellucida binding. Generally, GAPDHS was considered mainly as sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility and its role in the sperm head was unknown. In this study, we confirmed the potential additional role of GAPDHS as a binding protein that is involved in the sperm-zona pellucida interaction.
Keywords: GAPDHS; monoclonal antibody; sperm glycolytic enzyme; zona pellucida glycoprotein matrix
Project no.: GA P503/12/1834, ED1.1.00/02.0109
Funding provider: GA ČR
Host item entry: Book of abstracts of XXIst Symposium of biology and immunology of reproduction

Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0262906

Permalink: http://www.nusl.cz/ntk/nusl-260986


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Research > Institutes ASCR > Institute of Biotechnology
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