Název: Standards for capillary isoelectric focusing with laser induced fluorescence detection
Autoři: Šlais, Karel ; Nováčková, J. ; Friedl, Z.
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Symposium Separations in the BioSciences /2./, Praha (CZ), 2001-09-17 / 2001-09-20
Rok: 2001
Jazyk: eng
Abstrakt: Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.
Klíčová slova: capillary isoelectric focusing; laser induced fluorescent detection; standards for cIEF
Číslo projektu: CEZ:AV0Z4031919 (CEP), IAA4031901 (CEP)
Poskytovatel projektu: GA AV ČR
Zdrojový dokument: 2nd International Symposium Separations in the BioSciences. SBS 2001, ISBN 80-7080-437-8

Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0059619

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-24527


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference