Název: Dynamic modification of proteins for fluorometric detection in CZE
Autoři: Horká, Marie ; Šlais, Karel
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Symposium Separations in the BioSciences /2./, Praha (CZ), 2001-09-17 / 2001-09-20
Rok: 2001
Jazyk: eng
Abstrakt: The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
Klíčová slova: capillary zone electrophoresis; dynamic modification; fluorometric detection
Číslo projektu: CEZ:AV0Z4031919 (CEP), IAA4031901 (CEP)
Poskytovatel projektu: GA AV ČR
Zdrojový dokument: 2nd International Symposium Separations in the BioSciences. SBS 2001, ISBN 80-7080-437-8

Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0059618

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-24526


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference