Original title: Study of DNA oligonucleotides interactions with ethidium bromide by partial-filling affinity capillary electrophoresis
Authors: Růžička, Martin ; Koval, Dušan ; Kašička, Václav
Document type: Papers
Conference/Event: Advances in Chromatography and Electrophoresis & Chiranal 2014, Olomouc (CZ), 2014-02-10 / 2014-02-14
Year: 2014
Language: eng
Abstract: A partial filling affinity capillary electrophoresis was developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classical DNA intercalator ligand –ethidium bromide (EtBr). Binding constants of DNA EtBr complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths in hydroxypropylcellulose coated fused silica capillary. Binding constants of DNA-EtBr complex were found to be in the range 4.2 x 103 – 1.5 104 L/mol.
Keywords: affinity capillary electrophoresis; binding constant; oligonucleotide
Project no.: GAP206/12/0453 (CEP), GA13-17224S (CEP), GA13-32974S (CEP)
Funding provider: GA ČR, GA ČR, GA ČR
Host item entry: Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 51S, ISBN 978-80-244-3950-1, ISSN 0232-0061

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0235085

Permalink: http://www.nusl.cz/ntk/nusl-187442


The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers