Original title: Separation of nonpolar lipids from vernix caseosa
Authors: Háková, Eva ; Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef
Document type: Papers
Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year: 2014
Language: eng
Abstract: The aim of this work is to develop a separation method for characterization of unknown nonpolar lipids in vernix caseosa. Here we optimized adsorption chromatography coupled to mass spectrometry detection with exact mass measurement. A long silica gel column (250 + 250 x 4.6 mm, particle size: 5 mu m) provided the best separation of nonpolar lipids present in vernix caseosa.
Keywords: lipids; mass spectrometry; normal-phase chromatography; vernix caseosa
Project no.: GAP206/12/0750 (CEP)
Funding provider: GA ČR
Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0243322

Permalink: http://www.nusl.cz/ntk/nusl-180563


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers