Název:
Polymerase synthesis of new photocaged DNA
Autoři:
Vaníková, Zuzana ; Hocek, Michal Typ dokumentu: Příspěvky z konference Konference/Akce: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Rok:
2014
Jazyk:
eng
Abstrakt: 5-[(2-nitrobenzyl)oxymethyl]-2’-deoxyuridine 5’-O-triphosphate (dUNBTP) and 5-hydroxymethyl-2’- deoxyuridine 5’-O-triphosphate (dUHMTP) were incorporated to diverse DNA sequences by polymerase synthesis (PEX or PCR). UHM modified DNA was synthesized also through the photolysis of photocaged, UNB-linked DNA. The presence of bulky NB-modification in the recognition sequence of DNA resulted in blocking of cleavage by all restriction endonucleases (REs), however sequences with small HM-modification (formed after irradiation by UV) were perfectly cleaved by all enzymes. Using of photoremovable protecting group in the DNA sequence presents bioorthogonal chemical masking and it has a potential in gene manipulation and cloning.
Klíčová slova:
DNA; polymerase synthesis Číslo projektu: GBP206/12/G151 (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0
Instituce: Ústav organické chemie a biochemie AV ČR
(web)
Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0234534