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Název:
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
Autoři: Benkovská, Dagmar ; Flodrová, Dana ; Bobálová, Janette ; Laštovičková, Markéta
Typ dokumentu: Příspěvky z konference
Konference/Akce: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Rok: 2013
Jazyk: eng
Abstrakt: N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Klíčová slova: barley; glycosylation; N-glycopetptides; post-translational modification
Číslo projektu: EE2.3.20.0182, GPP503/12/P395 (CEP)
Poskytovatel projektu: GA MŠk, GA ČR
Zdrojový dokument: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770
Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na vyžádání prostřednictvím repozitáře Akademie věd.
Původní záznam: http://hdl.handle.net/11104/0228704
Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-170551
Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference
Autoři: Benkovská, Dagmar ; Flodrová, Dana ; Bobálová, Janette ; Laštovičková, Markéta
Typ dokumentu: Příspěvky z konference
Konference/Akce: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Rok: 2013
Jazyk: eng
Abstrakt: N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Klíčová slova: barley; glycosylation; N-glycopetptides; post-translational modification
Číslo projektu: EE2.3.20.0182, GPP503/12/P395 (CEP)
Poskytovatel projektu: GA MŠk, GA ČR
Zdrojový dokument: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770
Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na vyžádání prostřednictvím repozitáře Akademie věd.
Původní záznam: http://hdl.handle.net/11104/0228704
Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-170551
Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference
Záznam vytvořen dne 2014-03-13, naposledy upraven 2023-12-06.