Název:
Silica-based monolithic capillary columns modified to zwitterionic stationary phase for hydrophilic interaction liquid chromatography
Autoři:
Moravcová, Dana ; Planeta, Josef ; Kahle, Vladislav ; Horká, Marie ; Roth, Michal Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Rok:
2012
Jazyk:
eng
Abstrakt: Zwitterionic monolithic capillary columns intended for isocratic gradient hydrophilic interaction chromatography (HILIC) separations are introduced. Silica-based capillary columns (150 mm x 0.1 mm) were prepared by acidic hydrolysis of tetramethoxysilane in the presence of polyethylene glycol and urea. The modification by a 3-(trimethoxysilyl)propyl methacrylate and then by a zwitterionic [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide to HILIC stationary phase bearing sulfoalkylbetaine groups on its surface followed. Prepared columns were characterized in HILIC separation mode employing mobile phase containing 10% (v/v) of 5 mM ammonium acetate pH = 4.5 in acetonitrile. Comparison with the commercially available ZIC-HILIC® column (Merck SeQuant®) under the same separation conditions using a mixture of aromatic carboxylic acids as a sample was done on the basis of separation efficiency of tested columns as well as retention factors and peak asymmetry of individual solutes.
Klíčová slova:
HILIC; phenolic acids; zwitterionic stationary phase Číslo projektu: VG20112015021 (CEP), GAP206/11/0138 (CEP), GAP106/12/0522 (CEP) Poskytovatel projektu: GA MV, GA ČR, GA ČR Zdrojový dokument: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3 Poznámka: Související webová stránka: http://hdl.handle.net/11104/0215358
Instituce: Ústav analytické chemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v repozitáři Akademie věd. Původní záznam: http://hdl.handle.net/11104/0215358