Název: Micropreparative solution isoelectric focusing of peptides and proteins in nonwoven strip
Autoři: Duša, Filip ; Šlais, Karel
Typ dokumentu: Příspěvky z konference
Konference/Akce: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Rok: 2012
Jazyk: eng
Abstrakt: Recently we devised a new instrument for micropreparative analysis [1]. It is based on solution phase isoelectric focusing (sIEF) performed in a narrow channel (approximately 2 – 4 mm wide) with a strip of nonwoven fabric serving as the analysis bed. The strip can be precut before an analysis to make harvesting of fractions easier. Isoelectric focusing is driven by a programmable electrophoretic power supply. Progress of analysis is monitored by addition of colored isoelectric point (pI) markers. Usually sIEF runs are performed overnight. Evaporation of water is one of crucial features of analysis and it forces free liquid to shrink into the nonwoven fabric strip. Moreover, water evaporation increases liquid viscosity by altering ethylene glycol/water ratio. Fractions can be harvested simply by collecting and washing precut segments after the sIEF run. In this paper we used sIEF device for purification of caseinomacropeptide (CMP) from crude whey. Obtained fractions were further analyzed by HPLC. From acquired data a plot with chromatograms of all fractions was constructed to show purification profile of CMP. We proved that the new instrument is capable of quantitative purification of CMP complex from a globulin fraction.
Klíčová slova: isoelectric focusing; preparative; whey
Číslo projektu: VG20102015023 (CEP), EE2.3.20.0182
Poskytovatel projektu: GA MV, GA MŠk
Zdrojový dokument: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3
Poznámka: Související webová stránka: http://hdl.handle.net/11104/0215261

Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v repozitáři Akademie věd.
Původní záznam: http://hdl.handle.net/11104/0215261

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-135528


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Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference