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Emergency Button for Seniors
Verbík, Josef ; Chmelař, Milan (referee) ; Strašil, Ivo (advisor)
This Thesis „Emergency Button for Seniors“ is about suggestion of realization of system emergency signalization. The System consists of programmable digital watch with wireless interface and PC with software processing signals. Status signalization is created by phone message and figuration of dialog on monitor. There is used free software for realization. Operation system on PC is Linux (distribution Ubontu 11.04). For graphic transitional plane PC application is used cross-platform framework QT. For watch firmware is used C compiler MSP430-GCC, MSP debug v0,15 and firmware OpenChronos.
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Home security system - remote access module
Šarda, Petr ; Bradáč, Zdeněk (referee) ; Fiedler, Petr (advisor)
This thesis deals with remote access module for electric security systems (ESS). The design is based on valid standards that apply to a production of electric security system. It includes a design of module connection to the central that controls the module. There is also a communication protocol for connection with the central and with the user. The last step is development of web interface, which is realized on the selected development kit.
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Mobile Switching Centre Emulator
Králíček, Jan ; Ráb, Jaroslav (referee) ; Ščuglík, František (advisor)
This thesis attempts to provide basic overview of the topic of the GSM system and tries to design Mobile Switching Centre Emulator that could perform the operation Location Update. The first part of this thesis describes the principles and specifics of the GSM network (network structure, the operations required to support user mobility, network entities, etc.). The second part of this thesis describes the SS7 signaling protocols, SIGTRAN and MAP protocol. The final part deals with design of the emulator.
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Prediction and analysis of traffic crossings using machine learning
Hlaváček, Jakub ; Frýza, Tomáš (referee) ; Götthans, Jakub (advisor)
The thesis focuses on the area of traffic control, specifically traffic control of intersections, especially control technologies based on the obtained traffic data. The main goal of the work is the design and subsequent implementation of a real intersection Anenská and Capt. Jaroše, Pardubice in the form of a model. The work also deals with the demonstration of the used signal programs of junction control through the created model. The model is assembled from a wooden base and smaller parts are made by 3-D printing. The unit is controlled by an Arduino microcomputer. In creating this thesis, I managed to understand the comprehensive traffic management in today’s cities, which requires the current traffic situation.
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Possibilities of connecting the Asterisk softswitch to the 4th generation PBX
Halamík, Zdeněk ; Krajsa, Ondřej (referee) ; Šilhavý, Pavel (advisor)
This master’s thesis dissertate the possibilities of the linkage between Asterisk softswitch and the 4th generation private branch exchange. This should create a new generation’s network, so-called NGN, by the convergence of existing telecommunication networks with an IP computer network. This master’s thesis is divided into several chapters. In introduction is described the evolution of the private branch exchanges as well as the principles of the voice digitizing, codecs and signaling commonly used in both TDM and VoIP networks. The main aim of this project is the configuration of Asterisk software exchange for connection with PBX Alcatel 4400 as well as public phone network PSTN. Another goal of this master’s thesis was the configuration of Alcatel PBX and diagnostics of CCS and CAS signaling on E1 interface. In conclusion there are summarized advantages of NGN networks and their utilization in the future.
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Role of β-arrestin in μ-opioid and TRPV1 receptor signalling
Nagy Marková, Vendula ; Novotný, Jiří (advisor) ; Blahoš, Jaroslav (referee) ; Roubalová, Lenka (referee)
β-Arrestin belongs to the protein family which has a huge impact not only on GPCR signaling, but its role exceeds the function of the membrane channel, its own signaling cascade, or as a scaffold protein, etc. Here we aimed to study β-arrestin roles on the MOR behaviour in the plasma membrane or -opioid receptor (MOR) signaling and effect on adenylyl cyclase (AC) function using the siRNA to decrease the expression of β-arrestin isoforms. Furthermore, we focused on investigating the role of β-arrestin on the crosstalk between MOR and TRPV1 channels, which are important parts of pain transduction. For this purpose, we used HEK293 cells that stably expressed MOR-YFP or transiently transfected with TRPV1-CFP. We observed that both β-arrestin isoforms have an effect on the lateral mobility of MOR in the plasma membrane and the silencing of one or another β-arrestin isoforms abolishes the effect of MOR agonists to affect its diffusion in the plasma membrane. Interestingly, silencing of β-arrestin1 diminish the internalization of MOR induced by the endogenous agonist endomorphin-2. On the other hand, silencing of β-arrestin2 did not abolish the endomorphin-2 induced MOR internalization. Moreover, both isoforms exhibit a distinct impact on the inhibition of AC induced by the agonists of MOR....
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Physiological functioning of preptin and its analogs
Mrázková, Lucie ; Žáková, Lenka (advisor) ; Obšilová, Veronika (referee)
Preptin is a 34 amino acid long peptide that is derived from pro-IGF2, where it is cleaved from its E-domain and produced together with the matured IGF2. In my thesis we prepared two full-length preptins (mouse and human) and 8 preptin analogues (fragments with or without olefin metathesis). The full-length preptins as well as the preptin fragments were synthesized by solid-phase peptide synthesis. Mouse or human preptin served as our reference molecule for comparison with the results of bioassays of preptin analogues. All substances were tested for different biological activities to further elucidate the effect of preptin as a possible biologically active substance. We tested the effect on insulin secretion in pancreatic β-cells, indirectly in- vestigated the effect on bone cell proliferation and tried to approach the discovery of a possible receptor for preptin. From all our results we found that although preptin or some of its analogues have a higher effect than controls, the results are not consistent and the effect is not significantly higher. We were unsuccessful in our search for a receptor, despite having done a number of saturation experiments with radiolabelled preptin in many cell types. We also ruled out at the outset by binding experiments the possibility that preptin is able to bind...
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Role of the cannabinoid system in neurodegenerative processes
Akantisová, Lucia ; Novotný, Jiří (advisor) ; Búran, Peter (referee)
The endocannabinoid system (ECD) is involved in a significant number of physiological functions in the central and peripheral nervous system. The ECD consists of cannabinoid receptors (CB1 and CB2), endogenous ligands (ANA, 2-AG) and the enzymatic apparatus required for their synthesis (NAPE and DAGL) and degradation (FAAH, MAG). In the human brain, the cannabinoid receptor CB1 is the most widespread of the group of receptors that bind to G proteins. The signaling mechanisms of these proteins contribute to the overall homeostasis of the organism. With their activity, they affect the concentrations of second messengers, the activity of ion channels, the release of neurotransmitters, and regulate immune responses. In this context, studies in the last 20 years have focused on research in the field of neurodegenerative diseases. Today, i tis known that treatment with cannabinoid compounds improves neurological deficits associated with neuronal damage and alleviates inflammatory processes in animal models of Alzheimer's disease, Parkinson's disease and multiple sclerosis. At the clinical level, treatment with cannabinoids has helped with certain accompanying symptoms occuring in neurodegenerative diseases such as neuropathic pain, insomnia and spasticity. Key words: endocannabinoid system, cannabinoids,...
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Studies of cell signaling triggered by the TRH receptor
Trubačová, Radka
Activation of thyrotropin-releasing hormone receptor (TRH-R) signaling has an irreplaceable role in a number of cellular processes. Thyroliberin (TRH) plays an important role in the regulation of secretion of other hormones and there are also mentions of its possible antiapoptotic and neuroprotective effects. On the other hand, TRH is quickly degraded and also its other properties, such as cardiac and endocrine side effects and low lipophilicity, disadvantage the therapeutic applications of this hormone compared to its various analogues. Due to their effects on the central nervous systém, TRH and its analogues represent a potential possibility for the treatment of various neurological diseasses, including neurodegenerative disorders. The molecular mechanisms responsible for the beneficial effects of TRH and its analogues have not yet been fully elucidated. So far, little is known about the involvement of TRH, or its analogues, in the regulation of energy metabolism or impact on the cellular phosphoproteome. In the first part of the thesis, we focused on the study of the effect of TRH and interacting partners of TRH-R on the lateral mobility of this receptor in the membrane of the TRY-1 cell line stably expressing TRH-R labeled with yellow fluorescent protein (YFP) at the C-terminus. The results of...
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