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Integrated treatment of people with a dual diagnosis in the aftercare program: a series of case studies
Vít, Ondřej ; Volfová, Anna (advisor) ; Minařík, Jakub (referee)
Background: In the addiction treatment continuum, there is a relatively large group of clients with a dual diagnosis who repeatedly fail in treatment and follow-up treatment, with frequent relapses and recurrences of the disease and subsequent returns to the treatment continuum. The case study is focused on the perception of the integrated treatment of dual diagnosis in the follow-up care program by clients with dual diagnosis. Objectives: The research aims to map the perception of the integrated treatment of dual diagnosis in the aftercare service by the clients themselves. The sub-goals were to map the perception of the connection between addiction treatment and their mental disorder, what factors clients perceive as important in their journey to recovery, and how they perceive the presence of clients without mental illness. Methods: The theoretical part was processed in the form of a search of professional literature. The research part was carried out in the form of semi-structured interviews with three clients of the after-treatment program, the processing of their case reports, and their subsequent comparison. Research group: The research group consists of three clients of the after-treatment program selected by purposeful selection. The condition for participation in the research...
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Performance measuring of individuals with physical handicap
Vít, Ondřej ; Kálal, Jan (advisor) ; Radvanský, Jiří (referee)
The thesis is focused on the measurement of the energy consumption during the locomotion of the mobility disabled (riding a wheelchair a walking with 2 point support - crutch). The probands were handicapped by partial amputation of lower limb based on the vascular cause which represents approximately 90 percent of all amputations done in the Czech Republic. The thesis also presents a brief classification of amputations according to the extent of the separation, the history of amputations and the main etiologic factors leading to amputation. In the result section the measured data are discussed. In the last part of the thesis, the conclusions are discussed and compared to information available from similar studies. Powered by TCPDF (www.tcpdf.org)
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Studies of minor capsid proteins of the mouse polyomavirus
Vít, Ondřej ; Forstová, Jitka (advisor) ; Němečková, Šárka (referee)
Mouse polyomavirus (MPyV) is a small non-enveloped virus. Its capsid consists of 72 pentamers of the major capsid protein VP1. The central cavity of each VP1 pentamer contains one minor capsid protein, either VP2, or VP3. The minor capsid proteins are dispensable for capsid formation, but their presence is required for infection of the host cell, presumably because of their anticipated functions during virus entry. After internalization, MPyV virions traffic to endoplasmic reticulum (ER). VP2 and VP3 have been proposed to function as factors responsible for penetration of ER membranes, which is required for subsequent delivery of the viral DNA into the nucleus, a key step of the early phase of MPyV infection. Three hydrophobic domains were predicted in the sequence of VP2 and VP3. First in the unique Nterminal part of VP2, second and third in the common part of VP2 and VP3. The third domain corresponds to C-terminal VP1binding alpha-helix. It has been previously found in our laboratory, that VP2 and VP3 fused to N-terminus of EGFP, when expressed in mammalian cells, display properties similiar to the wild-type VP2 and VP3, namely affinity to intracellular membranes and high cytotoxicity. Expression plasmids carrying mutated VP2 and VP3 fused to Nterminus of EGFP were prepared to determine the hydrophobic...
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Proteomic analysis of soluble and transmembrane proteins in human lymphoma cells
Vít, Ondřej
In the works presented here, we studied molecular changes associated with drug resistance in human mantle cell lymphoma (MCL) cells using proteomics. Our analyses allowed us to identify causal and/or secondary changes in protein expression associated with the development of resistance to the experimental drug TRAIL and the clinically used antimetabolites cytarabine and fludarabine. Resistance of MCL cells to the recombinant proapoptotic cytokine TRAIL was associated with downregulation of key enzymes of purine metabolism. This pathway potentially represents a molecular weakness , which could be used as a therapeutic target for selective elimination of such resistant cells. Resistance to the pyrimidine analog drug cytarabine was associated with cross-resistance to other antinucleosides. Proteomic and transcriptomic analyses showed pronounced downregulation of deoxycytidine kinase (dCK), which activates both purine and pyrimidine antinucleosides. This change explains the cross-resistance and is the causal mechanism of resistance to cytarabine. Our observations suggest that MCL patients, who do not respond to cytarabine-based therapy, should be treated with non-nucleoside drugs. MCL cells resistant to purine-derived antinucleoside fludarabine were cross-resistant to all tested antinucleosides and also...
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Impact of membrane properties on clustering of transmembrane peptides
Sabó, Ján ; Cebecauer, Marek (advisor) ; Vít, Ondřej (referee)
Unfolded protein response (UPR) is a complex cellular mechanism induced upon ER stress caused by various environmental factors. Single spanning signal transducers of UPR were reported to recognise also lipid-induced ER stress. Studies of these transducers, namely PERK and IRE1 uncovered that they can sense change in membrane properties and activate themselves by clustering. Moreover, signal transducer IRE1 retained ability to sense changes in the membrane properties with TMD exchanged for a polyLeu α-helix. It was thus unclear what mechanism drives lipid-induced UPR via IRE1. We employed model membrane system in form of LUVs, where properties of membranes can be readily altered by specific lipid composition. As a simplified model of the UPR signal transducers in the ER, synthetic transmembrane peptides with polyLeu core were used. Dynamic light scattering (DLS) has been used for qualitative and semi-quantitative analysis of LUVs. Clustering of synthetic peptides was determined by time resolved anisotropy of fluorescence. DLS results demonstrate successful formation of vesicles with a desired size in all planned composition. On the contrary to the studies in living cells, the presence of cholesterol or palmitic acid in model membranes did not induce the aggregation of transmembrane peptides....
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Proteomic analysis of soluble and transmembrane proteins in human lymphoma cells
Vít, Ondřej
In the works presented here, we studied molecular changes associated with drug resistance in human mantle cell lymphoma (MCL) cells using proteomics. Our analyses allowed us to identify causal and/or secondary changes in protein expression associated with the development of resistance to the experimental drug TRAIL and the clinically used antimetabolites cytarabine and fludarabine. Resistance of MCL cells to the recombinant proapoptotic cytokine TRAIL was associated with downregulation of key enzymes of purine metabolism. This pathway potentially represents a molecular weakness , which could be used as a therapeutic target for selective elimination of such resistant cells. Resistance to the pyrimidine analog drug cytarabine was associated with cross-resistance to other antinucleosides. Proteomic and transcriptomic analyses showed pronounced downregulation of deoxycytidine kinase (dCK), which activates both purine and pyrimidine antinucleosides. This change explains the cross-resistance and is the causal mechanism of resistance to cytarabine. Our observations suggest that MCL patients, who do not respond to cytarabine-based therapy, should be treated with non-nucleoside drugs. MCL cells resistant to purine-derived antinucleoside fludarabine were cross-resistant to all tested antinucleosides and also...
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Proteomic analysis of soluble and transmembrane proteins in human lymphoma cells
Vít, Ondřej ; Petrák, Jiří (advisor) ; Šulc, Miroslav (referee) ; Lenčo, Juraj (referee)
In the works presented here, we studied molecular changes associated with drug resistance in human mantle cell lymphoma (MCL) cells using proteomics. Our analyses allowed us to identify causal and/or secondary changes in protein expression associated with the development of resistance to the experimental drug TRAIL and the clinically used antimetabolites cytarabine and fludarabine. Resistance of MCL cells to the recombinant proapoptotic cytokine TRAIL was associated with downregulation of key enzymes of purine metabolism. This pathway potentially represents a molecular "weakness", which could be used as a therapeutic target for selective elimination of such resistant cells. Resistance to the pyrimidine analog drug cytarabine was associated with cross-resistance to other antinucleosides. Proteomic and transcriptomic analyses showed pronounced downregulation of deoxycytidine kinase (dCK), which activates both purine and pyrimidine antinucleosides. This change explains the cross-resistance and is the causal mechanism of resistance to cytarabine. Our observations suggest that MCL patients, who do not respond to cytarabine-based therapy, should be treated with non-nucleoside drugs. MCL cells resistant to purine-derived antinucleoside fludarabine were cross-resistant to all tested antinucleosides and...
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Performance measuring of individuals with physical handicap
Vít, Ondřej ; Kálal, Jan (advisor) ; Radvanský, Jiří (referee)
The thesis is focused on the measurement of the energy consumption during the locomotion of the mobility disabled (riding a wheelchair a walking with 2 point support - crutch). The probands were handicapped by partial amputation of lower limb based on the vascular cause which represents approximately 90 percent of all amputations done in the Czech Republic. The thesis also presents a brief classification of amputations according to the extent of the separation, the history of amputations and the main etiologic factors leading to amputation. In the result section the measured data are discussed. In the last part of the thesis, the conclusions are discussed and compared to information available from similar studies. Powered by TCPDF (www.tcpdf.org)
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