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High-throughput organoid analysis platform for biomedical applications
Roček, Vojtěch ; Chmelíková, Larisa (referee) ; Janoušek, Oto (advisor)
Nowadays, the organoids structures has become more popular as suitable model systems for clinical research, particularly for development of new medication and drug screening. The standard study approaches include invazive biochemical or molecular-biology analysis as well as non-invasive optical approaches. Among optical methods, various microscopy techniques can give a very detailed information about the structure of organoids. However, the microscopy is time consuming as well as it puts a great demand on instrumentation. Therefore, the microscopy is not suitable for high content analysis of multiple samples. This work is focused on the development of the device and experimental technique for high-throughput screenings of organoids structures for biomedical applications based on microtitrate plates. Literatre search for non-invasive optical methods, suitable for analysis of organoid structures. The necessary adjustements of existing system for algae phenotypization are discussed. An experiment was made to test functionality of designed system. Practical use for clinical use is tested by the experiment of spheroids reaction to selected cytostatics. The results and findings are discussed in the conclusion.
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TiO2 nanoporous surfaces and their properties
Roček, Vojtěch ; Urbánková, Kateřina (referee) ; Drbohlavová, Jana (advisor)
This thesis is focused on creation nanostructured surfaces of titanium dioxide and the use of photocatalytic activity of these surfaces for antimicrobial applications. The work is divided into nine main chapters, in which the literature search for producing these surfaces, the principle of photocatalysis and their typical specifics was made. These theoretical findings are then used to form such layers, analyzing their chemical and physical properties and practical verification of their antimicrobial activity. This work experimentally verified processes to ensure the increase of antimicrobial activity. The results and findings are discussed in the conclusion.
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Biomolecules influence on optical properties of deposited quantum dots
Roček, Vojtěch ; Mravec, Filip (referee) ; Drbohlavová, Jana (advisor)
This thesis is focused on creation of quantum dots of titanium dioxide with help of nanoporous alumina mask and on funcionalization of their surface in purpose to increase the quantum yield of its. This thesis is devided into 6 main chapters, where the glossary topic of quantum dots creation process and ways of practical usage of these quantum dots are made. The theoretical knowledge is used for creation of titanium dioxide quantum dots, which was deposited by gold and funcionalized with glutation. The results are shown and discussed in the end of this work.
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High-throughput organoid analysis platform for biomedical applications
Roček, Vojtěch ; Chmelíková, Larisa (referee) ; Janoušek, Oto (advisor)
Nowadays, the organoids structures has become more popular as suitable model systems for clinical research, particularly for development of new medication and drug screening. The standard study approaches include invazive biochemical or molecular-biology analysis as well as non-invasive optical approaches. Among optical methods, various microscopy techniques can give a very detailed information about the structure of organoids. However, the microscopy is time consuming as well as it puts a great demand on instrumentation. Therefore, the microscopy is not suitable for high content analysis of multiple samples. This work is focused on the development of the device and experimental technique for high-throughput screenings of organoids structures for biomedical applications based on microtitrate plates. Literatre search for non-invasive optical methods, suitable for analysis of organoid structures. The necessary adjustements of existing system for algae phenotypization are discussed. An experiment was made to test functionality of designed system. Practical use for clinical use is tested by the experiment of spheroids reaction to selected cytostatics. The results and findings are discussed in the conclusion.
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Biomolecules influence on optical properties of deposited quantum dots
Roček, Vojtěch ; Mravec, Filip (referee) ; Drbohlavová, Jana (advisor)
This thesis is focused on creation of quantum dots of titanium dioxide with help of nanoporous alumina mask and on funcionalization of their surface in purpose to increase the quantum yield of its. This thesis is devided into 6 main chapters, where the glossary topic of quantum dots creation process and ways of practical usage of these quantum dots are made. The theoretical knowledge is used for creation of titanium dioxide quantum dots, which was deposited by gold and funcionalized with glutation. The results are shown and discussed in the end of this work.
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TiO2 nanoporous surfaces and their properties
Roček, Vojtěch ; Urbánková, Kateřina (referee) ; Drbohlavová, Jana (advisor)
This thesis is focused on creation nanostructured surfaces of titanium dioxide and the use of photocatalytic activity of these surfaces for antimicrobial applications. The work is divided into nine main chapters, in which the literature search for producing these surfaces, the principle of photocatalysis and their typical specifics was made. These theoretical findings are then used to form such layers, analyzing their chemical and physical properties and practical verification of their antimicrobial activity. This work experimentally verified processes to ensure the increase of antimicrobial activity. The results and findings are discussed in the conclusion.
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Getting rid of social networks addiction
Roček, Vojtěch ; Řepa, Václav (advisor) ; Jelínková, Veronika (referee)
The aim of this thesis is to find a method how to test the tools for an intervention with procrastination on social networks. The thesis tests and according to a built scale evaluates if there are people who are addicted to social networks. Further, the thesis introduces tools suitable for the intervention of this addiction and states distinct suggestions on how to perform a wider research to explore the most suitable tools. The thesis compares both the software tools centered on measuring, blocking, hiding and aiming, and psychological tools centered on how to work with oneself through calming down and mindfulness or creating a personal vision. The research is aimed at the PC and smart phones Android platforms. The outcome is a suggestion to investigate both tools individually described and their variable combinations during the intervention of the addiction to social networks to closely understand the effect of their using and practical implementation.
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Competitive Intelligence analyze of alternative energy market
Roček, Vojtěch ; Molnár, Zdeněk (advisor) ; Vejlupek, Tomáš (referee)
The main goal of this bachelor thesis was to analytically map part of alternative energy technology and services suppliers market in the Czech Republic based on the publicly available information. In the process, I tried to verify the division of the intelligence cycle into separate collecting and analyzing parts. To achieve this goal thirty five analytic documents were created by class 4IZ210 students in the winter semester of 2009. In these documents, the entities and the links between them were tagged using a macro developed by the Tovek company and processed in Analyst's Notebook. The result is a Topic Map containing around 2.000 entities and similar amount of links, which were adjusted and evaluated to formulate competitive intelligence conclusions. The structure of this thesis copies intelligence cycle, from the identification of the client's requirements, through data collecting to results analysis. At the end, some suggestions were made to help to increase the overall efficiency and optimize the intelligence cycle.
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