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Visualisation of alarms via OPC AE in the control system SIMOTION
Hrnčíř, Tomáš ; Hajátko, Pavel (referee) ; Zezulka, František (advisor)
In the Diploma Work is described the Simotion commanding system which has been produced by Siemens. Conception of the system and its features are explained. Further, the work deals with the data and alarm transfer among separate systems, an item which has been dealt with by the help of OPC (OLE for Process Control). Its update and specification have been covered with OPC Foundation organization. The alarm report visualization out of the Simotion system to WinnCC environment (which is not a client of OPC Alarms & Events server) is introduced here .
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The Role of Gut Microbiota and Lipopolysachaide Content of the Diet in the Development, Maturation and Function of the Immune System
Hrnčíř, Tomáš ; Tlaskalová - Hogenová, Helena (advisor) ; Prokešová, Ludmila (referee) ; Macela, Aleš (referee)
Mammals are essentially born germ-free but the epithelial surfaces are promptly colonized by astounding numbers of bacteria soon after birth. The most extensive microbial community is harboured by the distal intestine. The gut microbiota outnumbers ~10 times the total number of our somatic and germ cells. The hostmicrobiota relationship has evolved to become mutually beneficial. Studies in germfree mice have shown that gut microbiota is essential for the proper development of the immune system. The pivotal role of the innate immune system in the complex and dynamic host-microbiota interactions has become increasingly evident. The principal aims of the present study were: firstly, to determine whether LPS-rich sterile diet can promote maturation of the immune system in germ-free mice, secondly, to elucidate whether gut microbiota and LPS-rich sterile diet influence the LPS susceptibility, and finally, to investigate a role of the adaptive immunity in endotoxin shock. Our data clearly show that both live gut microbiota and LPS-rich sterile diet increase susceptibility to endotoxin shock. Further, we demonstrate that immunodeficient SCID mice, which lack mature B and T cells, are more sensitive to endotoxin shock than immunocompetent Balb/c mice. In addition, we show that not only live gut microbiota but also...
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Toll-like receptors in bacterial infections
Svobodová, Barbora ; Nachtigal, Petr (advisor) ; Hrnčíř, Tomáš (referee)
Bc. Barbora Svobodová Toll-like receptors in bacterial infections. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Aim: The aim of this diploma thesis was to determine the expression and the activation of the toll-like receptors (TLRs) 2, 4, 5 and 9 in bacterial infections by reverse transcription by real time polymerase chain reaction (RT-qPCR) and by Western blotting (WB). Another aim was to introduce detection of TLRs in a pig by WB in the Institute of Microbiology of the ASCR, v.v.i. in Nový Hrádek. Methods: 1) Optimization of WB - extraction of proteins, protein quantification, electrophoresis, Western blotting, immunodetection of proteins. 2) Four groups of one-week- old gnotobiotic piglets: germfree as a control (GF), piglets infected with Salmonella enterica serovar Typhimurium strain LT2 (LT2), piglets infected with Escherichia coli O55 (O55) and piglets colonized with Escherichia coli Nissle 1917 (EcN). 3) RT-qPCR - purification of a total RNA, spectrophotometric RNA quantification and estimation of purity, synthesis of cDNA, RT- qPCR, normalization and relativization of real-time PCR data. Results: WB analysis of TLR5 in the piglet ileum and mesenteric lymph nodes was optimized. Antibodies against TLR2, 4 and 9 were tested....
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The Role of Gut Microbiota and Lipopolysachaide Content of the Diet in the Development, Maturation and Function of the Immune System
Hrnčíř, Tomáš ; Tlaskalová - Hogenová, Helena (advisor) ; Prokešová, Ludmila (referee) ; Macela, Aleš (referee)
Mammals are essentially born germ-free but the epithelial surfaces are promptly colonized by astounding numbers of bacteria soon after birth. The most extensive microbial community is harboured by the distal intestine. The gut microbiota outnumbers ~10 times the total number of our somatic and germ cells. The hostmicrobiota relationship has evolved to become mutually beneficial. Studies in germfree mice have shown that gut microbiota is essential for the proper development of the immune system. The pivotal role of the innate immune system in the complex and dynamic host-microbiota interactions has become increasingly evident. The principal aims of the present study were: firstly, to determine whether LPS-rich sterile diet can promote maturation of the immune system in germ-free mice, secondly, to elucidate whether gut microbiota and LPS-rich sterile diet influence the LPS susceptibility, and finally, to investigate a role of the adaptive immunity in endotoxin shock. Our data clearly show that both live gut microbiota and LPS-rich sterile diet increase susceptibility to endotoxin shock. Further, we demonstrate that immunodeficient SCID mice, which lack mature B and T cells, are more sensitive to endotoxin shock than immunocompetent Balb/c mice. In addition, we show that not only live gut microbiota but also...
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Visualisation of alarms via OPC AE in the control system SIMOTION
Hrnčíř, Tomáš ; Hajátko, Pavel (referee) ; Zezulka, František (advisor)
In the Diploma Work is described the Simotion commanding system which has been produced by Siemens. Conception of the system and its features are explained. Further, the work deals with the data and alarm transfer among separate systems, an item which has been dealt with by the help of OPC (OLE for Process Control). Its update and specification have been covered with OPC Foundation organization. The alarm report visualization out of the Simotion system to WinnCC environment (which is not a client of OPC Alarms & Events server) is introduced here .
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Dismissals of employees for organizational changes
Hrnčíř, Tomáš ; Soušková, Milena (advisor) ; Spirit, Michal (referee)
The thesis deals with the dismissals of employees for organizational changes. This area of laubor law become very important in connection with the economic crisis beginning in the year of 2008. The main goal of this thesis therefore is, using various methods of law interpretation and relevant judicature, to find the content of very brief law regulation of artical 52 letter a) to c) Labour code. Finding that, the thesis could be used by the public as a manual for solving unclear law questions. The secondary aim of this disertation is to propose reasonable changes into the law regulation of dismissals of employees for organizational changes.
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Is Europol heading towards the "European FBI"?
Hrnčíř, Tomáš ; Druláková, Radka (advisor) ; Dubský, Zbyněk (referee)
The question whether EU should create its own parallel to the American FBI has been circulating in the society for several years. Similar discussions have strengthen even more after a new increase in global terrorism on the beginning of the new millennium. In this situation public started to focus almost exclusively on the European Police Office, an EU's agency founded in 1999 and commonly known as Europol.
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Vliv rekombinantní bakterie Lactobacillus plantarum na vývoj pylové alergieL
Schwarzer, Martin ; Repa, A. ; Wiedermann, U. ; Hrnčíř, Tomáš ; Daniel, C. ; Pot, B. ; Štěpánková, Renata ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Součková, Martina ; Kozáková, Hana
Germfree mice were monocolonized with recombinant Lactobacillus plantarum producing Bet v 1. Subsequently mice were sensitized by intraperitoneal injections to Bet v 1. We found out that monocolonization with recombinant L. plantarum reduced the level of specific Bet v 1 specific IgE antibody and stimulated production of INF-gamma in spleen cells supernatants. We conclude that monocolonization by recombinant L. plantarum shifts the immune response towards Th-1 direction and that it is a promising vaccine candidate against type I allergy
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