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Metafictional narrative strategies of two Cervantes' Exemplary Novels
Krátká, Tereza ; Holub, Jiří (advisor) ; Sánchez Fernández, Juan Antonio (referee)
The aim of the work is to sum up main function of the metafictional narrative strategies in two Cervantes' Exemplary Novels - The dialogue between Scipio and Berganze and The deceitful marriage. At the beginning, the text is focused on composition of both novels, mainly on their narrative structure. In the next part, the work is concentrated on analysis of reminiscences relevant - as for novels' genre, language and style - for interpretation of metaficitonal narrative with respect to the period of baroque upcoming. Then, the narrative structure is compared with Cervantes' novel Don Quijote which is based on the same type of narrative strategy. The author comes to the conclusion that the primary function of the metafictional narrative strategies is a possibility to interpret the question of the truth in relation to the baroque reality of the 17th century in Spain. The main importance of the work is presentation of a detailed narrative scheme of two novels, up to now absent in majority of theoretical works aimed at Exemplary Novels composition. Key words: Miguel de Cervantes, Dialogue between Scipio and Berganze, El coloquio de los perros, metafiction, intertextuality, credibility, narrative strategies.
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Use of technical hemp extracts in hair cosmetics
Benková, Sarah ; Holub, Jiří (referee) ; Márová, Ivana (advisor)
Technical hemp as a versatile plant has found applications in many sectors of the industry. In cosmetics, it is mainly used for bioactive substances as well as proteins. The subject of this bachelor thesis was the preparation and characterization of a fraction containing hydrolysed hemp proteins and its use in hair cosmetics. In the theoretical part, the characteristics of the plant as well as its chemical composition are processed. Attention is paid especially to hemp proteins, their hydrolysis possibilities, and the subsequent use of hydrolysates in hair cosmetics. Within the experimental part, the preparation of hemp protein hydrolysates from hempseed cake of the Santhica 70 variety was optimized using the industrial enzymes TS-E 1930 and TS-E 1993 and the subsequent determination of the protein content and profile of prepared hydrolysates. The carbohydrate, chlorophyll, phenolic and flavonoid content of the analysed hydrolysates was also determined. A hair conditioner was prepared from the selected hydrolysate and tested by ten respondents. Thanks to the prepared conditioner, hair cosmetics with the addition of hydrolysed hemp proteins has been shown to enhance shine and pliability of the hair, as well as help the healing of frayed ends.
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Production of pates with the addition of mushrooms
Tóthová, Martina ; Holub, Jiří (referee) ; Mikulíková, Renata (advisor)
This bachelor thesis deals with the sensory evaluation of pâté with mushrooms. First the 3 samples of pâtés were made to select the right concentration of mushrooms for sensory evaluation. Then the 7 samples of pâtés which contained different texture and types of mushrooms were produced. In the theoretical part pâtés are characterised, their manufacturing and types of pâtés are described. The part of literature search is description of mushrooms in general, their composition and description of mushrooms which were used for production of pâtés. Another topic is description of conditionals and methods of sensory analysis. The experimental part is focused on the production of pâtés for two sensory analyses, evaluating the heat treatment and sensory analysis. The content of vitamin D in the samples of pâtés was analysed by liquid chromatography. Processing of results of sensory analysis of pâtés was determined that the best samples were pâtés with milled champignons and champignons which were add in blender.
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Body and sexuality in San Camilo 1936
Šatopletová, Petra ; Sánchez Fernández, Juan Antonio (advisor) ; Holub, Jiří (referee)
In the Bachelor's thesis Body and Sexuality in the Novel San Camilo 1936, we are going to pursue some of the principal themes of this work; these are based on depictions of the human body in its physical form, i.e. disease, injury, death, external appearance of the characters, and various aspects of sexuality. In order to get to analyze the respective themes, it is neccessary to get to know the author first. It's Camilo José Cela, often noted as one of the greatest novelists of the 20th century, a winner of the Nobel Prize for Literature in 1989 and various other awards. Beside novels, Cela also wrote poetry, travelogs, plays, essays, short stories and newspaper articles. He is famous for his novel La Familia de Pascual Duarte (The Family of Pascual Duarte), introducing the new literary trend tremendism to the world of literature. The term tremendism is derived from the Spanish word 'tremendo', which can be translated as terrible, horrifying, raising fear etc. This by itself gives us an idea about this style's characteristics, which we are going to present in the following text. We consider it essential to give some insight into the period of history the novel was written and set in. It takes place at the very beginning of the Spanish Civil War, specifically the week around the 18th July 1936,...
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Self-rated health in geo-demographic context
Láchová, Jitka ; Dzúrová, Dagmar (advisor) ; Holub, Jiří (referee)
Master thesis tries to analyze self-rated health in the Czech Republic during the period of transformation. In total, individual data for 7827 respondents from three health interview surveys (years 1996, 2002 and 2008), were analyzed. Significant improvement of self-rated health status in the Czech population was identified. Factors as gender, age, education, economic activity, physical activity, body mass index and social contacts were confirmed as influencing factors of self-rated health in the Czech population. Significant results were found also for marital status, smoking and alcohol consumption, but opposite trends for different age groups were noticed. Eating habits and rural vs. urban differences do not significantly influence self-assessing health. Regional differences in self-rated health were not found in the Czech Republic at NUTS III level. Keywords: self-rated health, influencing factors of self-rated health, regional differences, transformation
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Luis Cernuda - The Melancholic Poet Tossed beetwen the Reality and the Desire.
Juračková, Pavlína ; Sánchez Fernández, Juan Antonio (advisor) ; Holub, Jiří (referee)
Palabras clave Cernuda - Melancolía - Generación del 27 - Moderna - Vanguardia Sumario La tesis reflexiona sobre la poética del libro La realidad y el deseo del poeta español y crítico literario Luis Cernuda. Dicho libro contiene seis colecciones que reflejan la maduración sucesiva de una sensibilidad. El trabajo va a través de las reflexiones generales hasta la circunstancia histórica cernudiana presentada por los conceptos de la vanguardia y la modernidad, a los cuales intenta acercarse y describirlos. Consecuentemente el trabajo avanza desde el contexto más amplio hasta el espacio español, a la Generación del 27, y luego a la meditación sobre cada una de las colecciones. El objetivo de las reflexiones es una introducción del poeta Luis Cernuda que se acerca con su poética y su vida privada a la grandeza de los poetas europeos, a los cuales premeditadamente elige como una fuente de su inspiración. Los poemas de Cernuda se incorporan, por un lado, a la tradición de la lírica moderna europea cavilada sobre la melancolía y la tristeza y, por otro lado, nos muestran unas originales reflexiones líricas que piensan en el destino humano en general. El trabajo podría ser un impulso para la traducción de la obra cernudiana al checo porque todavía sigue faltando en el espacio literario de nuestro país.
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Application of chemical and physical stresses in the late phase of growth to selected strains of microorganisms
Langer, Marek ; Holub, Jiří (referee) ; Szotkowski, Martin (advisor)
A wide spectrum of microorganisms is used in the biotechnological industry for production of meaningful metabolites; including carotenoids and chlorofyles with antioxidant effects, fatty acids, ubiquinone and ergosterol. Ways to increase yield of metabolites to maximum capacity are being researched to increase efficiency and economical sustainability in industry. One of the ways is the application of chemical stress factors on microorganisms as is described in this thesis. In the theoritical part certain microorganisms, their important intracellular metabolites and their metabolism are established. The last subchapter covers the usage of stress factors in industry. In the experimental part each microorganism was subjected to various concentrations of sodium chloride, cobalt(II) sulfate and iron(III) chloride. Significant increase of lipid production was registered after an application of sodium chloride on yeast strain Rhodosporium toruloides. An increase in production of torularhodin in Sporidiobolus pararoseus occured after application of iron salt. After an application of sodium chloride the growth of torularhodin in strains Cystofilobasidium macerans a Rhodosporidium toruloides was significant. Sodium chloride was also a suitable stress factor for lycopene formation in Rhodotorula mucilaginosa. The most meaningful stress factor of algae was cobalt(II) sulfate which increased metabolite production in Scenedesmus obliquus. Cyanobacteria Anabaena torulosa also attained a significant production in the presence of sodium chloride.
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Testing of biological effect of glucans on cell lines in vitro
Šimková, Eva ; Holub, Jiří (referee) ; Němcová, Andrea (advisor)
The aim of this bachelor thesis is isolation and testing of -glucans from selected strains of yeast. In the theoretical part, characterisation of -glucans, microbial production of -glucans by yeast, determination of polysaccharides, exopolysaccharides and in vitro testing are described. Yeast have a cell wall made up of -glucans and -glucans are known for their healing properties. In the experimental part of this work deals with characterization of selected biomass yeasts in different forms and comparison of their effects and the amount of -glucans in each form. Stains of Metschnikowia pulcherrima, Cystofilobasidium infirmominiatum and Rhodotorula kratochvilovae have been selected for this thesis. Subsequently, the thesis deals with the isolation and testing of isolated -glucans from a selected lyophilised strain of yeast Rhodotorula kratochvilovae. The selected strain was isolated in 2 ways. The first method of isolation that was more friendly to conformation of -glucans due to the effect of ultrasonic homogenization of cells. The second method of isolation was less friendly to -glucans, using acid and base to homogenise them. Analyses of both extraction methods confirmed the presence of isolated -glucans, however it was found that the recovery of both methods was relatively low and requires further optimisation of the procedure. Overall, the better extraction procedure was the second method. A sample of this extract was further tested on the HaCaT cell line. The results of the pilot tests confirmed the cytotoxicity of the extract obtained by us.
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Optimization of extraction of metabolites produced by selected strains of microalgae and carotenogenic yeast.
Obračaj, Jan ; Holub, Jiří (referee) ; Szotkowski, Martin (advisor)
This bachelor thesis is focused on the production of microbial biomass using selected strains of cyanobacteria and carotenogenic yeasts. Subsequent preparation of extracts and study of their stability under different temperature conditions with the addition of the antioxidant tocopherol. In the experiment, 2 series of extracts with hexane and ethanol were prepared. The theoretical part of the work describes the studied metabolites carotenoids, chlorophylls, ubiquinone, ergosterol and microbial lipids. Furthermore, the used strains of cyanobacteria and carotenogenic yeasts are described here. Finally, methods for extraction and analysis of metabolites are described. The experimental part describes the used cultivation techniques, preparation of microbial extracts and used analytical methods. The results part of the work then contains a summary of the results of long-term stability tests of extracts. The results of the work clearly confirm the temperature dependence of the stability of individual metabolites. This phenomenon was most evident in chlorophyll and carotenoid pigments. Hexane appears to be the best solvent in terms of the stability of the substances used. The results of this work provide important information in terms of the economics of storage of these valuable substances usable in the food, pharmaceutical or feed industries.
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