National Repository of Grey Literature 44 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Quality of life evaluation in pregnant women in the Czech republic and Slovakia by means of a specific questionaire QOL-GRAV
Bujňáková, Terézia ; Rathouská, Jana (advisor) ; Hronek, Miloslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of diploma thesis: Quality of life evaluation in pregnant women in the Czech republic and Slovakia by means of a specific questionaire QOL-GRAV Author: Terézia Bujňáková Supervisor: PharmDr. Jana Rathouská, Ph.D. Backround First, an overview of the theory concerning quality of life, pregnancy and folic acid intake was the part of the main aims of this thesis. Experimental section is focused on evaluating quality of life of pregnant women, supplementation of folic acid before and during gravidity and its potential effect on quality of life of women in the Czech republic and Slovakia, evaluated by a specific questionaire QOL-GRAV. Methods We used a quntitative method in the form of specific questionaire QOL-GRAV, in which questions about folic acid supplementation and eating habits changes before and during pregnancy, were implemented. 564 pregnant women from Czech and Slovak republic were questioned in any week of gravidity or two weeks after delivery, at latest. Results We can consider quality of life of pregnant women in Czech and Slovak republic as very good, according to average value 18,81 points of 45 points overall obtained from the questionaire, where minimum possible value of 9...
Atopic dermatitis ańd progress in therapy
Krymová, Lucie ; Rathouská, Jana (advisor) ; Konečná, Klára (referee)
Atopic dermatitis is a multifactorial chronic inflammatory disease which is one of the most common skin diseases. The prevalence is relatively high and is still growing. In former times, atopic dermatitis was considered primarily a childhood disease but today, the prevalence is increasing in adult patients too. Atopic dermatitis is a chronic incurable disease with a significant negative impact on patients' lives. The negative impact on the quality of life often leads to a lack of adherence of these patients to the standard treatment procedure, which implies the need to combine different therapies in the field of complementary and alternative medicine. While there is still not enough data available to conclude that adjuvant therapies are effective in the conventional sense, there are already promising results that suggest that these therapeutic strategies might be an alternative to treating this disease. Although mild and moderate forms of disease can be well controlled using external treatments, such as emollients, topical corticosteroids, and local calcineurin inhibitors, the treatment of severe atopic dermatitis is still a major challenge. Systemic treatment with corticosteroids is a relatively effective treatment in many cases, but cannot be used for a long time due to numerous side effects. And...
Voluntary vaccination in children
Sejkorová, Ilona ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Bc. Ilona Sejkorová Voluntary vaccination in children Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods Many viruses or bacteria may be the cause of infectious diseases. Is possible to use vaccination against some of them. Voluntary vaccination in childeren is still an actual topic. Even though, vaccination has many opponents, it still belongs to the most effective preventive precautions. Objectives: The thesis consists of a theoretical summary of information related to child voluntary vaccination problems. The practical part consists of a questionnaire study whose goal was to determine the attitude of parents and potential parents to child optional vaccination topic. Also, to find out the awareness of the population about the topic, to assess the most used voluntary vaccination, and possibly further information about dangerous side effects of the voluntary vaccinations. Methods: A questionnaire study for a period October 2018 to February 2019 in the Czech Republic. The questionnaire consisted of 20 questions directed to parents and potential parents of various ages (606 respondents). Results: Most of the respondents (94 %) were aware about a possibility of the child voluntary vaccination. More than a half (51 %) of respondents was informed about...
Drug allergies - immunopathological reactions of skin and mucous membranes
Švecová, Monika ; Rathouská, Jana (advisor) ; Kovařík, Miroslav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medicinal Science Consultant: PharmDr. Jana Rathouská, Ph.D. Candidate: Monika Švecová Title of diploma thesis: Drug allergies - immunopathological reactions of skin and mucous membranes Immune reactions to small molecular compounds as drugs can cause a variety of diseases mainly involving skin, mucous membranes, liver, kidney, lungs and other organs. In addition to relatively well-known immediate and IgE mediated reactions to drugs, many drug - induced hypersensitivity reactions appear delayed. These reactions are predominantly characterized by exanthematous macular or maculopapular skin eruptions. However, approximately 2 % of cases develop into a severe and life - threating systematic immune reaction associated with organ damage. Severe/life - threatening reactions include bullous reactions, such as Stevens - Johnson syndrome, toxic epidermal necrolysis, but also acute generalized exanthematous pustulosis and drug reaction with eosinophilia and systemic symptoms. Advances in the understanding of the pathophysiology of these reactions is based on the understanding of the mechanisms by which drugs are recognized by T lymphocytes, on a thorough analysis of subtypes and function of CD4+ and CD8+ T cells...
Pathophysiology of Metabolic syndrome and current therapeutic approaches.
Mohamed Hamdy, Engy ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
The goal of this thesis is the knowledge and analysis of the Metabolic Syndrome and its association with the cardiovascular diseases. Aspects such as epidemiology, pathophysiology, and complications will be discussed in this thesis. The general and known pharmacological and non-pharmacological approaches towards the syndrome will be analyzed. It is also discussed in this thesis the new therapeutic approaches towards the syndrome, with the benefits and toxicities listed.
Antigenic structures of erythrocytes
Anderlová, Petra ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Background: The knowledge of antigens expressed on red blood cells is an integral part of a transfusion medicine that uses this knowledge for effective and more safe hemotherapy. The aim of this work is to create an overview of selected important blood group systems and to compile existing knowledge into one document. Main fingings: Most antigenic structures on erythrocytes are included in group systems that provide better lucidity and orientation. Each group system contains both antigens and relevant antibodies, whose importance is clinically specific. Expression of the antigens on erythrocyte is always given by genetics, so it is possible to find links between belonging to some blood group and appearance of some diseases. Conclusions: There have been sought information about individual group systems, their antigens and their antibodies. In antigens especially about their structure and genetic background, in antibodies especially about their particular reactivity and clinical relevance. These already existing facts have been compared with some selected clinical trials and there has also been referred to associations of blood antigens with the occurrence of some pathological conditions. Keywords: antigen, antibody, blood group system, blood transfusion
Depression and its risk factors
Sejkorová, Monika ; Rathouská, Jana (advisor) ; Kudláčková, Zděnka (referee)
Background: Thesis consists of theoretical summary of information which characterizes depression. Practical part consists of questionnaire study, whose goal is to determine the most important factors, which take part in growth of depression disease. As well to determine the effect of season on depression progress, the most common age when depression starts to develop, eventually some knowledge about a character and treatment of depression. Methods: Questionnaire study from November 2017 to April 2018, which consists of 22 questions for respondents from Czech Republic (850 respondents of whom 291 were diagnosed depression). Results: More than a half of respondents (53 %) diagnosed with depression have chosen two and more risk factors. Thus, depression appears based on combination of more factors. The most frequently are mentioned ill family relationships (20 %) and tragic event in life (20 %). It is also negatively influenced by season, mostly autumn (46 % of answers) and winter (33 % of answers). The most common age when the depression starts to appear is 11-20 years. Most frequently respondents were diagnosed moderate level of depression (60 %) and they have chosen to visit a doctor out of their own belief (57 %). The main signs are tiredness (18 %) and loss of interest and enjoyment of activities...
Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice
Čermáková, Martina ; Rathouská, Jana (advisor) ; Blažíčková, Kateřina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice Author of diploma thesis: Bc. Martina Čermáková Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: The aim of this diploma thesis was to evaluate expression of selected oxidative stress molecules (SOD-3, iNOS, catalase, HO-1) in cardiac aorta and nitrate and nitrite levels in plasma in two groups of mice. These two groups of mice varied in genetic features - the monitored group was, compared to control group of mice, deficient for apolipoprotein E and LDL receptor. Methods: For the study, 2 months old male mice were fed a standard rodent diet. The males were divided into two groups - control (C57BL/6J) and monitored (apoE-/- /LDLR- /- ). Western blot method was used to evaluate and quantify iNOS, SOD-3, catalase and HO-1 molecules in the aorta. HPLC method was used to quantify nitrate and nitrite levels in plasma. Result: HPLC method did not show statistically significant differences in the nitrate and nitrite levels in plasma between the two groups of examined mice. Western blot analysis of aortic samples showed statistically significantly higher SOD-3 expression (208.39 %) and a significantly...
Attitude of mothers to compulsory vaccination in children
Červinková, Pavla ; Rathouská, Jana (advisor) ; Konečná, Klára (referee)
Bc. Pavla Červinková Attitude of mothers to compulsory vaccination in children Charles University, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods The most effective prevention of serious infectious diseases is vaccination. In the Czech Republic, there is a compulsory vaccination against nine diseases, which have not been eradicated in the world yet. The topic of compulsory vaccination is currently a very topical and discussed issue. Objectives: Theoretical overview of the issue of compulsory vaccination in children, individual diseases, the possible risks of vaccination and available vaccines. The aim of the questionnaire study is to determine the percentage of mothers with a negative attitude to this issue and their reasons for their attitude. Methods: Questionnaire study within the Czech Republic. The questionnaire was intended for women - mothers of different age categories. Results: In the general public, there is a wide range of views on this issue. Most of mothers have a positive attitude towards compulsory vaccination. A significant part of mothers point to a bad legislation in the Czech Republic. They particularly criticize the vaccination scheme and the obligation of using combined vaccines, in which mothers often observe side effects. Conclusion: The questionnaire...
Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...

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