National Repository of Grey Literature 24 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Treatment of spinal cord injury targetting secondary mechanisms
Valášková, Barbora ; Jendelová, Pavla (advisor) ; Hájek, Milan (referee) ; Rokyta, Richard (referee)
1 Abstract Traumatic spinal cord injury (SCI) is severe condition with devastating long- lasting consequences. There is still no available treatment. After initialing mechanical trauma, there is a huge cascade of secondary reactions which amplifies the damage. This thesis is focused on potential therapeutic effect of photobiomodulation, natural anti- inflammatory compounds epigallocatechin-3-gallate (EGCG), curcumin, their combination and extrapure synthetic form of curcumin called "nanocurcumin" on impacts of experimental model of SCI in rats. Photobiomodulation using combination of two synchronized wavelengths 808 and 905 nm improved functional recovery after SCI evaluated by battery of locomotor tests and somatosensory plantar test. The group treated by photobiomodulation obtain better results in all tests. The histopathological analysis showed a positive effect on white and gray matter sparing and our data suggests an upregulation of M2 microglia/macrophages in photobiomodulation treated rats assessed by immunohistochemical and RT-qPCR analysis. Our results demonstrated that the photobiomodulation is a promising non-invasive therapy for improving functional recovery and tissue sparing after SCI. EGCG and curcumin are natural compounds known in Chinese medicine for centuries. Their neuroprotective and...
Modulation of memory using enzymatic digestion of perineuronal nets in perirhinal cortex
Procházková, Natálie ; Jendelová, Pavla (advisor) ; Chmelová, Martina (referee)
In the adult brain, neuronal plasticity is regulated by a specialized structure of extracellular matrix, the perineuronal nets (PNNs), which restrict synaptic plasticity by binding molecules of inhibitory nature and posing as a physical barrier to alterations in neuronal connectivity. This effect is abolished by removal of PNNs by the enzyme chondroitinase ABC (chABC), which enables to reopen critical period window and leads to memory improvement. Here, we utilized chABC and a novel approach in removing the PNNs in perirhinal cortex, using the enzyme hyaluronidase (Hyase), to assess differences in the use of these enzymes in object recognition (OR) memory improvement and alterations in the structure of neuronal network of wild type mice. Our findings suggest that Hyase may be a more convenient tool to PNN removal than chABC, as Hyase surpasses chABC in promoting the OR memory, influence larger portion of neuronal network by affecting both inhibitory and excitatory neurons, and provides extended temporal window for experimental modulation of activity-dependent synaptic plasticity. Key words: perineuronal nets, synaptic plasticity, chondroitinase ABC, hyaluronidase, perirhinal cortex, memory
Cellular and molecular basis of a skin regeneration in amphibians and mammals
Hybešová, Michaela ; Krylov, Vladimír (advisor) ; Jendelová, Pavla (referee)
Wound healing of skin in mammals and its regeneration in Amphibians are crucial biomedicine topics in the last few decades. The most important aspect in humans is the scrarring proccess and the effort to substitute it with the regeneration producing functional and differentiated tissues. To modulate the formation of scar it is neccessary to compare both proccesses. The core animal model is axolotl (Caudata) where regeneration takes place during whole lifespam. On the other hand, in frogs (Anurans) this phenomenon is restricted up to metamorphosis. After metamorphosis, the immune system of Anurans is similar if compared to mammals. Similarly, the transition from early embryonic development in mammals, where the fetus was able to completely regenerate damaged tissue to an adult type of healing, goes hand-in-hand with the development of the immune system and structural differentiation of damaged tissue. Thus, the inflammatory cells and their regulation, the formation of ECM, which includes fibroblast proliferation, and the production of appropriate cytokines are key factors that distinguish the process of healing with and without scarring.
Jiráková, Klára ; Moskvin, Maksym ; Horák, Daniel ; Jendelová, Pavla
Cells labelled with iron oxide nanoparticles (ION) can be tracked by magnetic resonance imaging (MRI) in several applications. However, various studies demonstrated toxicity and oxidative stress induction associated with nanoparticles exposure. We analysed biologic effects after the exposure of two types of iron oxide nanoparticles (with and without an antioxidative agent; an ascorbic acid) on human neural stem cells. The labelled cells in gel phantoms were detected in MRI and they showed decreased relaxation rates in comparison with control. ION slightly decreased cell proliferation in comparison with unlabelled cells, which was dependent on concentration and presence of ascorbic acid. None of the nanoparticle type showed negative effect on cell viability and both demonstrated minor effect on reactive oxygen species (ROS) formation. Unfortunately, ascorbic acid bound to nanoparticles did not show any effect on ROS attenuation. Cells exposed to both types of nanoparticles showed increased positivity for a phosphorylated form of H2AX a marker of double strand breaks. We showed that ION in low concentrations do not affect cell viability, but have negative effect on cells on DNA level. Their potential use for oxidative stress reduction is dependent on the concentration of ascorbic acid bound to the nanoparticles and this should be further increased.
Anti-inflammatory compounds and stem cells in treatment of spinal cord injury
Kárová, Kristýna ; Jendelová, Pavla (advisor) ; Sameš, Martin (referee) ; Chvátal, Alexandr (referee)
Despite intense scientific efforts, spinal cord injury (SCI) remains to be a severe neurological condition that has no treatment. Currently, therapy is based on alleviating pressure by surgical spinal cord decompression, administration of methylprednisolone and physical therapy. In this study, therapeutic effects of anti-inflammatory compounds and of three types of stem cells were tested in a balloon compression model of spinal cord injury in rats. Natural compounds epigallocatechin-3-gallate (EGCG) or curcumin were administered in situ and then intraperitoneally every day for up to 28 days. Human bone marrow mesenchymal stem cells (MSCs), human spinal neural precursors (SPC-01) and neural precursors derived from human induced pluripotent stem cells (iPS-NPs) were transplanted intrathecally (MSCs) or via spinal injection into immunosuppressed rats 7 days after induction of SCI. To determine effects of therapies, changes in motor function was tested by open field test BBB, flat beam test and score, Plantar test and rotarod. Morphometric analysis was used to assess gray/matter sparing and cavity size. Immunohistochemistry was used to determine survival and differentiation of transplanted cells, activation of classical pathway of NFκB (p65 nuclear translocation), astroglial activation (GFAP) and...
Cellular Reprogramming as a Tool for Harvesting Patient-specific Stem Cells
Pisal, Rishikaysh ; Mokrý, Jaroslav (advisor) ; Hampl, Aleš (referee) ; Jendelová, Pavla (referee)
Cellular reprogramming as a tool for harvesting patient specific stem cells In the year 2006, Dr. Yamanaka surprised the entire field of medicine, by reporting a technique of inducing pluripotency in somatic cells. In his article, he had displayed that fibroblasts could be reprogrammed to pluripotent stem cell state, by ectopic expression of four transcription factors namely OCT4, SOX2, c-MYC and KLF4. His discovery made a paradigm shift in the field of reprogramming because previous methods of reprogramming were dependent on use of human oocytes and this raised ethical concerns. Moreover, his technique of cellular reprograming broadened the spectrum of application of somatic cells in regenerative medicine. Objectives of my research were focused on; development of an optimised protocol for detection of mycoplasma that commonly infects animal tissue culture; detailed characterization of reprogrammed clones; targeted differentiation of iPSC towards myogenic lineage, and construction of an expression vector, optimised for miRNA expression. For detecting mycoplasma infection, we adapted the protocol of Uphoff et al. (2002). By skipping the DNA extraction step (reported in the original protocol) and instead directly using cell culture supernatant and a robust polymerase enzyme for performing PCR, we...
Studium interakce buněk s biomimetickým materiálem a jeho využití v biomedicíně
Sauerová, Pavla ; Hubálek Kalbáčová, Marie (advisor) ; Vandrovcová, Marta (referee) ; Jendelová, Pavla (referee)
Biomaterials are considered as very promising tools for regenerative medicine. They have compensatory or supporting function in organism and they are often developed to support specific conventional medical procedures. So-called biomimetic materials are developed to imitate natural environment of organism and to induce positive innate responses of organism. An essential part of biomaterial development is in vitro biological evaluation, which characterizes (often for the first time) the potential of developed material for its clinical application. This Ph.D. thesis deals with in vitro biological evaluation of three different biomimetic materials. In all three cases, the comprehensive evaluation was an integral part of the material development and optimization processes. Each material was in vitro characterised at the level of cell-material interactions with respect to its intended specific application.. In the first part, cell response to potential drug delivery system based on colloidal complexes of cationic surfactants with hyaluronic acid (HyA) was characterized. HyA protection ability and its limits were described; also the role of fetal bovine serum (FBS) in cell response to the stress stimuli was confirmed. Results considered surfactant-HyA complexes as promising system for drug delivery. In...
Central nervous system plasticity in adulthood and after injury.
Procházková, Natálie ; Jendelová, Pavla (advisor) ; Chmelová, Martina (referee)
Perineural nets are a structure of extracellular matrix, enwrapping several subpopulations of neurons in the central nervous system. Their formation is linked to the closure of critical period and significant plasticity restriction. In a healthy organism, they are important for stabilisation of mature nervous system, support of highly active neurons, and neuroprotection. However, they are one of the factors that restrict tissue regeneration during pathological conditions by participating in the formation of glial scar and upregulating molecules that have inhibitory impact on neuron sprouting. Digestion of perineuronal nets, which is mostly achieved enzymatically, leads to re-opening of critical period and renewal of plasticity, potentiating neuronal sprouting and growth and overall regeneration of central nervous system after mechanical damage, such as spinal cord injury, or during neurodegenerative diseases, as is Alzheimer's disease. Perineural nets play a similar role in Alzheimer's disease and aging, where they participate in memory loss. Renewal of plasticity in these conditions leads to facilitation of synaptic transmission and therefore eliminating the memory deficit. Key words: neurodegenerative diseases, CNS injury, Alzheimer's disease, perineural nets, chondroitinsulfate proteoglycans
The role of link proteins in the stabilization of the brain extracellular matrix and in formation and maintaining of the perineuronal nets
Suchá, Petra ; Vargová, Lýdia (advisor) ; Jendelová, Pavla (referee)
The brain extracellular space (ECS) contains specified macromolecules forming the extracellular matrix (ECM), containing a high amount of negative charges that could bind water or other soluble ions and molecules diffusing within the ECS. In specific brain areas, the ECM molecules form a condensed, reticular-like structure of perineuronal nets (PNNs). It has been found that PNNs appear at the end of the critical period, when they stabilize the synapses and terminate their plasticity and may have also neuroprotective function. To study the role of brain link protein 2 (Bral2) in stabilizing the ECM complexes, we employed the real-time iontophoretic method and immunohistochemical analysis to show the difference in the ECS diffusion parameters and level of expression of the ECM molecules between the wild type and Bral2-deficient mice. We also compared changes in the ECS diffusion parameters induced by Bral2 deficiency with those appeared after enzymatic destruction of the ECM by the chondroitinase ABC (chABC). In the Bral2-deficient mice, we discovered significantly decreased values of tortuosity in the trapezoid body. This difference was age related and did not manifest itself in young mice. Immunohistochemical analysis showed that inferior colliculus does not contain Bral2-brevican based...
The biocompatibility and potential cytotoxicity of materials for joint replacement manufacturing and coating
Kopová, Ivana ; Bačáková, Lucie (advisor) ; Hubálek Kalbáčová, Marie (referee) ; Jendelová, Pavla (referee)
Currently used prostheses for total joint replacement still have numerous disadvantages: extreme stiffness or elastic modulus of the bulk metallic material; insufficient integration of the implant into the host bone; and a high wear and corrosion rate, which causes an accumulation of mostly metallic or polymeric wear debris. Because of these reasons, many patients experience increasing local pain, swelling, allergic reactions, and inflammation resulting in bone loss and the aseptic loosening of the implant leading to the need for painful and expensive revision surgery. To address the mechanical issues of commonly used orthopaedic alloys, this thesis presents the development of the new β-type titanium alloy Ti-35Nb-7Zr-6Ta-2Fe-0.5Si with a relatively low elastic modulus (up to 85 GPa), increased tensile strength (880 MPa), and enhanced biocompatibility and osteoconductivity. Considering the generally low osteoinductivity of metallic implants, various surface modifications and coatings have been developed to improve the cell-material interaction, e.g. carbon-based coatings. Among these coatings, C60 fullerene layers have emerged as a great candidate for coating orthopaedic implants due to their therapeutic potential in arthritis. The potential cytotoxicity and DNA damage response of fullerenes have...

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