Název: Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides
Autoři: Neubauerová, Tereza ; Macková, Martina ; Macek, Tomáš ; Šanda, Miloslav ; Voburka, Zdeněk
Typ dokumentu: Příspěvky z konference
Konference/Akce: Biologically Active Peptides /12./, Praha (CZ), 2011-04-27 / 2011-04-29
Rok: 2011
Jazyk: eng
Abstrakt: The antibiotic resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our laboratory, several peptide fractions with antimicrobial acivity against gram-positive and gramnegative bacteria and fungi were isolated from larvae of flesh fly Neobellieria bullata. The presence of peptides was proved by tricine electrophoresis, molecular weights and sequences determination by MS and Edman sequencing, respectively. In comparison with database sequences we obtained structures present mainly in insect and amphibian antimicrobial peptides, but total matches were not higher than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Antimicrobial activity of these peptides was determined.
Klíčová slova: antimicrobial peptide; Neobellieria bullata; peptide synthesis
Číslo projektu: CEZ:AV0Z40550506 (CEP), GA522/09/1693 (CEP), GD305/09/H008 (CEP)
Poskytovatel projektu: GA ČR, GA ČR
Zdrojový dokument: Biologically Active Peptides. 12th Conference, ISBN 978-80-86241-44-9

Instituce: Ústav organické chemie a biochemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0204344

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-81017


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav organické chemie a biochemie
Konferenční materiály > Příspěvky z konference