Název:
Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Autoři:
Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef Typ dokumentu: Příspěvky z konference Konference/Akce: International Students Conference "Modern Analytical Chemistry" /7./, Prague (CZ), 2011-09-29 / 2011-09-30
Rok:
2011
Jazyk:
eng
Abstrakt: Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
Klíčová slova:
digalactosylglycerols; high-resolution mass spectrometry; monogalactosyldiacylglycerols; RP-HPLC Číslo projektu: CEZ:AV0Z40550506 (CEP), SVV261204, GA203/09/0139 (CEP) Poskytovatel projektu: GA KU, GA ČR Zdrojový dokument: Proceedings of the 7th International Students Conference "Modern Analytical Chemistry", ISBN 978-80-7444-010-6
Instituce: Ústav organické chemie a biochemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0201265