Vliv glukózy na expresi biomarkerů endotelové dysfunkce u endotelových buněk.

Schmidtová, Kristína

Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Glucose effects on the expression of biomarkers of endothelial dysfunction in endothelial cells Author: Kristína Schmidtová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this diploma thesis was to determine whether the new monoclonal antibody carotuximab affects the expression of endoglin and its transcription factors (KLF6 and RELA), as well as the expression of enzyme MMP-14 and soluble endoglin, in human aortic endothelial cells that were exposed to high glucose levels over the period of 3 days. Methods: We used human aortic endothelial cells (HAEC), passage 5, and we exposed them to high glucose levels (45 mmol) for the period of 3 days and to carotuximab (300 μg/ml) for 12 hours. The results were compared with a control group which was exposed to normal glucose levels (5 mmol). Real-time PCR was used to measure the mRNA expression of endoglin, KLF6, RELA and MMP-14. Protein levels of endoglin and the enzyme MMP-14 were measured by flow cytometry. ELISA method was used to measure the level of soluble endoglin. Results: Gene expression of endoglin, transcription factors and the enzyme MMP-14 was significantly increased after...

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