guest ::
| Home > Conference materials > Papers > Deformation response of polydimethylsiloxane substrates subjected to uniaxial quasi-static loading |
Original title:
Deformation response of polydimethylsiloxane substrates subjected to uniaxial quasi-static loading
Authors: Vinařský, V. ; Martino, F. ; Forte, G. ; Šleichrt, Jan ; Rada, Václav ; Kytýř, Daniel
Document type: Papers
Conference/Event: Youth Symposium on Experimental Solid Mechanics /17./, Telč (CZ), 20190606
Year: 2019
Language: eng
Abstract: To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. PDMS based material is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore, we adapted recently available protocol aiming to prepare substrates which offer stiffness in physiological range 5−100 kPa using various mixtures of Sylgard. An in-house developer loading device with single micron position tracking accuracy and sub-micron position sensitivity was adapted for this experimental campaign. All batches of the samples were subjected to uniaxial loading. During quasi-static experiment the samples were compressed to minimally 40% deformation. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated.
Keywords: hyperelasticity; in-situ loading device; polydimethylsiloxane substrates; quasi-static loading
Project no.: ATCZ133
Host item entry: Acta Polytechnica CTU Proceedings, ISBN 978-80-01-06670-6
Note: Související webová stránka: https://ojs.cvut.cz/ojs/index.php/APP/article/view/6032
Institution: Institute of Theoretical and Applied Mechanics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0296581
Permalink: http://www.nusl.cz/ntk/nusl-395935
The record appears in these collections:
Research > Institutes ASCR > Institute of Theoretical and Applied Mechanics
Conference materials > Papers
Authors: Vinařský, V. ; Martino, F. ; Forte, G. ; Šleichrt, Jan ; Rada, Václav ; Kytýř, Daniel
Document type: Papers
Conference/Event: Youth Symposium on Experimental Solid Mechanics /17./, Telč (CZ), 20190606
Year: 2019
Language: eng
Abstract: To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. PDMS based material is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore, we adapted recently available protocol aiming to prepare substrates which offer stiffness in physiological range 5−100 kPa using various mixtures of Sylgard. An in-house developer loading device with single micron position tracking accuracy and sub-micron position sensitivity was adapted for this experimental campaign. All batches of the samples were subjected to uniaxial loading. During quasi-static experiment the samples were compressed to minimally 40% deformation. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated.
Keywords: hyperelasticity; in-situ loading device; polydimethylsiloxane substrates; quasi-static loading
Project no.: ATCZ133
Host item entry: Acta Polytechnica CTU Proceedings, ISBN 978-80-01-06670-6
Note: Související webová stránka: https://ojs.cvut.cz/ojs/index.php/APP/article/view/6032
Institution: Institute of Theoretical and Applied Mechanics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0296581
Permalink: http://www.nusl.cz/ntk/nusl-395935
The record appears in these collections:
Research > Institutes ASCR > Institute of Theoretical and Applied Mechanics
Conference materials > Papers
Record created 2019-06-11, last modified 2021-11-24