Název: Deformation response of polydimethylsiloxane substrates subjected to uniaxial quasi-static loading
Autoři: Martino, F. ; Vinařský, V. ; Šleichrt, Jan ; Kytýř, Daniel
Typ dokumentu: Příspěvky z konference
Konference/Akce: Youth Symposium on Experimental Solid Mechanics /17./, Telč (CZ), 20190606
Rok: 2019
Jazyk: eng
Abstrakt: To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. Polydimethylsiloxane (PDMS) based material Sylgard 184 is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore we adapted recently published protocol [1] aiming to prepare substrates which offer stiffness in physiological range 5−100 kPa using various mixtures of Sylgard 527 and Sylagard 184. The inhouse developed loading device with the loading capacity of 3 kN with 1 μm position tracking accuracy and sub-micron position sensitivity was employed for this experimental campaign. The experiments were controlled by the proprietary LinuxCNC software running on the real-time kernel [2]. All batches of the samples were subjected to monotonic compression loading. During the displacement driven experiment with loading rate 10 μm · s−1 the samples with diameter 12.00 ± 0.05mm and height 14 − 16mm were compressed to minimally 50% deformation. Because of high differences in the samples stiffness various load cells with nominal capacity 50N, 10N, and 1N was used for the most reliable force logging. Material properties for all batches were derived from a set of tests under dry and simulated physiological conditions. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated as secant up to 10% deformation.
Klíčová slova: compression loading; hyperelasticity; polydimethylsiloxane substrates
Číslo projektu: ATCZ133
Zdrojový dokument: Book of Abstracts. XVIIth Youth Symposium on Experimental Solid Mechanics, ISBN 978-80-86246-45-1

Instituce: Ústav teoretické a aplikované mechaniky AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0296581

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-395935

Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav teoretické a aplikované mechaniky
Konferenční materiály > Příspěvky z konference
 Záznam vytvořen dne 2019-06-11, naposledy upraven 2019-06-11.

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