Original title: A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
Authors: Ďurč, P. ; Lačná, J. ; Dosedělová, V. ; Foret, František ; Homoľa, L. ; Pokojová, E. ; Malá, M. ; Skřičková, J. ; Dastych, M. ; Vinohradská, H. ; Dřevínek, P. ; Skalická, V. ; Kubáň, Petr
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.
Keywords: capillary electrophoresis with contactless conductometric detection; cystic fibrosis; method for diagnostic of cystic fibrosis
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0290884

Permalink: http://www.nusl.cz/ntk/nusl-390732


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