Original title: Locking in on large volume light-sheet microscopy
Authors: Vettenburg, T. ; Dalgarno, H.I.C. ; Nylk, J. ; Coll-Lladó, C. ; Ferrier, D.E.K. ; Čižmár, Tomáš ; Gunn-Moore, F.J. ; Dholakia, K. ; Corral, A. ; Rodriguez-Pulido, A. ; Flors, C. ; Ripoll, J.
Document type: Papers
Conference/Event: Recent Trends in Charged Particle Optics and Surface Physics Instrumentation, Skalský dvůr (CZ), 20180604
Year: 2018
Language: eng
Abstract: Fluorescence light-sheet microscopy is increasingly adopted by developmental biologists to study how cells divide and differentiate to form organs and even entire organisms. The lightsheet microscope differs from a conventional microscope in that the specimen is illuminated by a plane of light orthogonal to the detection axis, thus keeping the out-of-focus areas dark while minimizing any potentially detrimental exposure of the sample. The light-sheet microscope has been found to be the ideal instrument for long-term and non-invasive studies of intact, and therefore three-dimensional, fluorescent samples.
Keywords: cellular imaging; fluorescence imaging; light-sheet microscopy
Host item entry: Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Proceedings of the 16th International Seminar, ISBN 978-80-87441-23-7

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0287640

Permalink: http://www.nusl.cz/ntk/nusl-387518


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