Original title: Using actinomycete-specific PCR primers to characterize the bacterial communities of wormcasts
Translated title: Použití specifických aktinomycetových PCR primerů k charakterizaci bakteriálních společenstev exkrementů žížal
Authors: Hill, P. ; Krištůfek, Václav ; Feijoo, A. M. ; Gallego, G.
Document type: Papers
Conference/Event: Contributions to soil Zoology in Central Europe I. Central European Workshop on Soil Zoology /7./, České Budějovice (CZ), 2003-04-14 / 2003-04-16
Year: 2005
Language: eng
Abstract: [eng] [cze]
Tropical geophageous earthworms stimulate soil bacterial activity in soil that they ingest; it is uncertain if they affect the whole bacterial population or only subgroups. We sampled .i.Martiodrillus heterostichon, Polypheretima elongata./i. and bulk soil from a Bamboo plantation near Cali, Colombia. Pots of 1500 g of an adjacent tobacco field soil were brought to field capacity and two worms of each species placed in each pot. .i.Martiodrillus./i. gut contents and wormcasts from the soil surface and within tunnels were sampled after three months. The soil was maintained at field capacity for a further five months during which the worms continued to produce casts. Worm cast Amplified ribosomal DNA restriction analysis ARDRA (using Tag I) with actinomycete specific primers gave two strong bands at approximately 750 and 370 bp. ARDRA patterns from the three bamboo grove and four field soils had several additional bands notably at 140 and 126 bp. Studie se zabývala tropickými žížalami - .i.Martiodrillus heterostichon, Polypheretima elongata./i.. Tyto druhy žížal stimulují aktivitu bakterií v půdě, kterou požírají; není dosud známé, zda ovlivňují celou bakteriální populaci nebo pouze sub-skupiny. Technikami ARDRA, BLAST A DNAMAN byla zjištěna selekce skupiny aktinomycetů - .i.Nocardioides./i. - v průběhu trávení půdy sledovanými druhy geofágních žížal.
Keywords: actinomycetes; Colombia; earthworms
Project no.: CEZ:AV0Z6066911 (CEP), SL18 (CEP)
Funding provider: CIAT
Host item entry: Contributions to Soil Zoology in Central Europe I. Proceedings of the 7th Central European Workshop on Soil Zoology, ISBN 80-86525-04-X

Institution: Institute of Soil Biology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0018088

Permalink: http://www.nusl.cz/ntk/nusl-20846


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Research > Institutes ASCR > Institute of Soil Biology
Conference materials > Papers