Original title: Analysis of low abundant lipids in vernix caseosa using chromatographic methods and mass spectrometry
Authors: Háková, Eva ; Míková, R. ; Vrkoslav, Vladimír ; Plavka, R. ; Cvačka, Josef
Document type: Papers
Conference/Event: International Students Conference "Modern Analytical Chemistry" /11./, Prague (CZ), 2015-09-22 / 2015-09-23
Year: 2015
Language: eng
Abstract: The aim of this work is to develop a separation method for characterization of unknown lipids in vernix caseosa. We focused on very low abundant lipids which have not been previously described in vernix caseosa. The total lipid extract was fractionated using low pressure normal phase liquid chromatography to obtain sub-fractions. The sub-fractions were further separated in high performance liquid chromatography mode to isolate classes of lipids. This procedure enabled us to characterise lipid classes by mass spectrometry and identify one of the class as acyl-ceramides with non-hydroxylated fatty acids.
Keywords: high performance liquid chromatography; lipids; mass spectrometry; vernix caseosa
Project no.: GAP206/12/0750 (CEP)
Funding provider: GA ČR
Host item entry: Proceedings of the 11th International Students Conference "Modern Analytical Chemistry", ISBN 978-80-7444-036-6

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0250419

Permalink: http://www.nusl.cz/ntk/nusl-200746


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers