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Astrocyte volume changes during brain ischemic injury
Mikešová, Michaela ; Anděrová, Miroslava (advisor) ; Zemková, Hana (referee)
Brain ischemic injury is a complex of pathophysiological events following transient or permanent reduction of brain blood flow. It results in a disruption of neuronal and astrocytic physiological functions, long-term reduction of brain blood flow leads to the cell death. Number of recent studies is focused on astrocytes, which might play key roles in surviving cells, including neurons, during ischemic injury. Astrocytes provide many important functions, such as maintenance of ionic homeostasis, prevention of excitotoxicity, scavenging free radicals and others and thus astrocytes may dramatically swell during ischemic conditions and contribute notably to the development of cytotoxic edema. This thesis summarizes mechanisms possibly contributing to the astrocytic swelling during brain ischemic injury as well as methods used for studying astrocyte volume changes and their quantification. Since the brain edema dramatically complicates both course and treatment of ischemic injury, knowledge of mechanisms leading to astrocytic swelling and their volume regulation during ischemia/reperfusion might be used for developing new therapeutic approaches for the treatment of cerebral ischemia, mainly for reduction of negative impact of edema.
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Astrocyte volume changes in alpha-syntrophin deficient mice
Mikešová, Michaela ; Anděrová, Miroslava (advisor) ; Vargová, Lýdia (referee)
(EN) The formation of brain oedema, which accompanies ischemic or traumatic brain injuries, originates from a disruption of ionic/neurotransmitter homeostasis that leads to extracellular K+ elevation and neurotransmitter accumulation in the extracellular space. An increased uptake of these osmotically active substances, predominantly provided by astrocytes, is accompanied by intracellular water accumulation via aquaporin-4 (AQP4). Since it has been shown that the removal of perivascular AQP4 via the deletion of α- syntrophin, which is the protein responsible for anchoring AQP4 on the astrocytic membrane (Neely et al. 2001), delays oedema formation and K+ clearance (Amiry-Moghaddam et al. 2003), we aimed to elucidate how the alpha-syntrophin deletion affects astrocyte volume changes in the cortex during pathological states, such as hypoosmotic stress or oxygen- glucose deprivation (OGD), using three-dimensional (3D) confocal morphometry in situ. In order to visualize individual astrocytes that lack alpha-syntrophin, double transgenic mice (GFAP/EGFP/α-Syn-/- ) were generated by crossbreeding GFAP-EGFP mice with α- syntrophin knockout mice. 3D-confocal morphometry revealed that alpha-syntrophin deletion did not alter astrocyte swelling during hypoosmotic stress or their recovery in isotonic solution;...
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Work in a children's team - Activities supporting the development of relationships in a children's group
Mikešová, Michaela ; Pařízek, Michal (advisor) ; Kaplánek, Michal (referee)
The topic of this bachelor thesis is a children's group, group relation-ships and their development with the help of physical activities, focusing on affective competencies. This thesis is about children's group limited by age from 3 to 6 years. This age is characterized in the thesis. Areas of development of a preschool child are presented here and they are divided into subchapters, where the thesis deals with motor skills, and psycho-social, emotional and moral development.It deals with the child's environment and activities from various areas. Factors that affect children's relationships are also mentioned. The bachelor thesis deals with educational goals, including affective ones, explains the concepts of value and attitude. Thereafter, it defines activities and games in kin-dergarten. Finally, the bachelor thesis focuses on implementation of the games. The games are chosen deliberately and their goal is to support the development ofaffective competencies and thus the development of relationships in the children's group. There are methodological cardsin the attachments, according to which the selected games can be easily implemented. Keywords Physical activities, Games, Affective competencies, Attitudes, Values, Moral development, Relationships in the children's group
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Marketing Mix in a chosen Building Firm
Mikešová, Michaela ; Vlnas, Pavel (advisor) ; Harantová, Monika (referee)
The subject of this thesis is processing of the marketing mix in selected building company SAHAN CB Ltd., which is based in České Budějovice. The main aim of this thesis is analysis of the marketing mix in this company and suggestion of potention improvements. The thesis is divided into four chapters. The first and the second chapter form the theoretical part of this thesis and summarize the problem of marketing, services marketing, marketing in the building industry and marketing mix. In the third chapter is determine the aim of the thesis, hypotheses and the basic research methods. The last part is focused on the introduction of the building company SAHAN CB Ltd., its marketing environment and marketing mix.
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Astrocyte volume changes in alpha-syntrophin deficient mice
Mikešová, Michaela ; Anděrová, Miroslava (advisor) ; Vargová, Lýdia (referee)
(EN) The formation of brain oedema, which accompanies ischemic or traumatic brain injuries, originates from a disruption of ionic/neurotransmitter homeostasis that leads to extracellular K+ elevation and neurotransmitter accumulation in the extracellular space. An increased uptake of these osmotically active substances, predominantly provided by astrocytes, is accompanied by intracellular water accumulation via aquaporin-4 (AQP4). Since it has been shown that the removal of perivascular AQP4 via the deletion of α- syntrophin, which is the protein responsible for anchoring AQP4 on the astrocytic membrane (Neely et al. 2001), delays oedema formation and K+ clearance (Amiry-Moghaddam et al. 2003), we aimed to elucidate how the alpha-syntrophin deletion affects astrocyte volume changes in the cortex during pathological states, such as hypoosmotic stress or oxygen- glucose deprivation (OGD), using three-dimensional (3D) confocal morphometry in situ. In order to visualize individual astrocytes that lack alpha-syntrophin, double transgenic mice (GFAP/EGFP/α-Syn-/- ) were generated by crossbreeding GFAP-EGFP mice with α- syntrophin knockout mice. 3D-confocal morphometry revealed that alpha-syntrophin deletion did not alter astrocyte swelling during hypoosmotic stress or their recovery in isotonic solution;...
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Astrocyte volume changes during brain ischemic injury
Mikešová, Michaela ; Zemková, Hana (referee) ; Anděrová, Miroslava (advisor)
Brain ischemic injury is a complex of pathophysiological events following transient or permanent reduction of brain blood flow. It results in a disruption of neuronal and astrocytic physiological functions, long-term reduction of brain blood flow leads to the cell death. Number of recent studies is focused on astrocytes, which might play key roles in surviving cells, including neurons, during ischemic injury. Astrocytes provide many important functions, such as maintenance of ionic homeostasis, prevention of excitotoxicity, scavenging free radicals and others and thus astrocytes may dramatically swell during ischemic conditions and contribute notably to the development of cytotoxic edema. This thesis summarizes mechanisms possibly contributing to the astrocytic swelling during brain ischemic injury as well as methods used for studying astrocyte volume changes and their quantification. Since the brain edema dramatically complicates both course and treatment of ischemic injury, knowledge of mechanisms leading to astrocytic swelling and their volume regulation during ischemia/reperfusion might be used for developing new therapeutic approaches for the treatment of cerebral ischemia, mainly for reduction of negative impact of edema.
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Management of marketing at the University of South Bohemia in České Budějovice
MIKEŠOVÁ, Michaela
The particular purpose of my thesis was to analyze University of South Bohemia in České Budějovice?s marketing management. In my thesis I have focused on marketing communication and issues of corporate identity which belongs to fundamental components of public relations. After evaluation of instruments currently put to use and after benchmarking elaboration I can convey, however, University of South Bohemia falls behind other universities in many aspects particularly in modern trends. My thesis summarizes many deficiencies and areas which deserves more attention in the future. I believe the University is going to realize measures to improve its position. I also hope it will appreciate my comments and it will take my proposals into account which could catch up some of trends.
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Human Resource Management within the postal services
MIKEŠOVÁ, Michaela
The subject of my Bachelor{\crq}s thesis is Human Resource Management within the Česka Posta State Company. The purpose of my thesis is to evaluate the particular processes involved in human resource management, as well as examining the competition in the labour market. I have mainly focused upon the analysis of the activities of the Česka Posta State Company Personnel Department. It specifically regards the motivation and personal satisfaction of the employees of the Česka Posta State Company. The analysis of the results has demonstrated that this company has not neglected the management of its human resources.
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