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Estimation of the Point Radiation Source Activity Based on the Airborne Spectrometry
Jurečka, Tomáš ; Chromý, Adam (referee) ; Lázna, Tomáš (advisor)
The thesis deals with the localization of gamma-ray source and an estimation of its radiation activity with airborne spectrometry. In the theoretical parts are presented the types of ionizing radiation, the principles of measurement of gamma-ray by scintillation detectors and the explanation of airborne spectrometry. A revision of algorithms for data processing from aviation spectrometry was performed. In the practical part, the sensitivity of scintillation counter was determined depending on the height above the calibrated source. The mathematical methods for locating and estimating activity of the searched emitter were verified.
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Detection and classification of flying objects
Jurečka, Tomáš ; Richter, Miloslav (referee) ; Janáková, Ilona (advisor)
The thesis deals with the detection and classification of flying objects. The work can be divided into three parts. The first part describes the creation of dataset of flying objects. The reverse image search is used to create the dataset. The next part is a research of algorithms for detection, tracking and classification. Subsequently, the individual algorithms are applied and evaluated. In the last part, the design of hardware components is performed.
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Metabolism of cabozantinib by enzymes of first phase of biotransformation
Jurečka, Tomáš ; Indra, Radek (advisor) ; Kubíčková, Božena (referee)
Cabozantinib is an anticancer drug that inhibit tyrosine kinases which allow signal pathways important for growth and development of tumors. It is used for treatment of medullary thyroid cancer, hepatocellular carcinoma and kidney cancer. The major enzymes of the first phase of biotransformation that metabolize cabozantinib are cytochromes P450. In this thesis it was studied metabolism of cabozantinib and cytochromes P450 that participated on this metabolism. Hepatic microsomes of rat, mouse and rabbit were used for studying metabolism of cabozantinib in this thesis. It was also focused on the impact of particular isoforms of cytochromes P450 on metabolism of cabozantinib in rat microsomes. Time dependence of cabozantinib conversion in hepatic rat microsomes was also studied. Enzyme kinetics of metabolism of cabozantinib in hepatic rat microsomes, as well as impact of cytochromes P450 inhibitors on the metabolism were included. Metabolites were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. Formation of metabolites of cabozantinib increased over time to 30 minutes of incubation and with some others to 40 minutes of incubation. Up to five different metabolites were detected in experiments (M1, desmethyl cabozantinib, M3, monohydroxy cabozantinib and cabozantinib...
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Detection and classification of flying objects
Jurečka, Tomáš ; Richter, Miloslav (referee) ; Janáková, Ilona (advisor)
The thesis deals with the detection and classification of flying objects. The work can be divided into three parts. The first part describes the creation of dataset of flying objects. The reverse image search is used to create the dataset. The next part is a research of algorithms for detection, tracking and classification. Subsequently, the individual algorithms are applied and evaluated. In the last part, the design of hardware components is performed.
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Estimation of the Point Radiation Source Activity Based on the Airborne Spectrometry
Jurečka, Tomáš ; Chromý, Adam (referee) ; Lázna, Tomáš (advisor)
The thesis deals with the localization of gamma-ray source and an estimation of its radiation activity with airborne spectrometry. In the theoretical parts are presented the types of ionizing radiation, the principles of measurement of gamma-ray by scintillation detectors and the explanation of airborne spectrometry. A revision of algorithms for data processing from aviation spectrometry was performed. In the practical part, the sensitivity of scintillation counter was determined depending on the height above the calibrated source. The mathematical methods for locating and estimating activity of the searched emitter were verified.
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Metabolism of tyrosine kinase inhibitor cabozantinib by rat liver microsomes
Jurečka, Tomáš ; Indra, Radek (advisor) ; Mrízová, Iveta (referee)
Cabozantinib is an anticancer drug that was approved for treatment of progressive thyroid cancer by FDA and EMA organizations. Cabozantinib is a tyrosine kinase inhibitor. It blocks signal pathway receptors that are important for growth of tumors. This bachelor's thesis describes the findings about the metabolism of cabozantinib. It studies metabolism of cabozantinib in hepatic microsomes isolated from various laboratory animals (rat, mouse and rabbit) and impact of particular isoforms of cytochromes P450 (CYP) on metabolism of cabozantinib in rat hepatic microsomes. The bachelor's thesis also describes the optimization of method for separation metabolites of cabozantinib by high performance liquid chromatography (HPLC) and also identification of metabolites using mass spectrometry. Up to three different metabolites were detected by utilizing hepatic microsomes isolated from various laboratory animals. Those were M1, monohydroxycabozantinib and O-desmethylcabozantinib. Mouse microsomes oxidized cabozantinib mainly to O-desmethylcabozantinib and rabbit microsomes metabolised cabozantinib mainly to monohydroxycabozantinib. Microsomes from controlled rats produced two metabolites with the overall majority of monohydroxycabozantinib. The highest number of metabolites was produced by microsomes from...
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