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The use of immunochemical methods in the diagnostic of cancer and degenerative disease
Hanousková, Lenka ; Kotaška, Karel (advisor) ; Bronský, Jiří (referee) ; Štern, Petr (referee)
Introduction: Immunochemical methods have significant importance in biomedical research. The benefit of immunochemical methods is their high sensitivity and accuracy. The aim of the work was to use immunochemical methods in investigation of cancer biomarkers and degenerative diseases. A total of four studies were performed. Two studies were focused on the search for new biomarkers of prostate cancer, the third study was focused on evaluation of FGF23 in patients with hyperparathyroidism, and the fourth study looked at the assessment of hyaluronic acid in synovial fluid of patients with knee gonarthrosis. Methods and patients: Study 1: Concentrations of Chromogranin A, Endoglin, TIMP-1 and thymidine kinase 1 (TK1) in serum and Endoglin, SPINK-1, Annexin, TIMP-1 in urine were measured in 58 patients with the (mean age 68 years, range 45 - 82 years) with clinically diagnosed prostate cancer and in 30 healthy individuals with the (mean age 64 years, range: 55 - 78 years) without clinically relevant urological history and PSA values in the reference range. Subsequently, serum TK1 levels were measured in 169 patients (mean age 62 years, range 45- 82 years) with prostate cancer and in a control group of 39 healthy men (mean age 61 years, range 54-78 years). The TK1 assay was also used to determine the...
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Disagreement about childbirth
Hanousková, Lenka ; Šanderová, Jadwiga (advisor) ; Soukup, Petr (referee)
This thesis deals with disagreement between midwives and doctors about the fact where and in which conditions mothers should give birth to a child. While the doctors support the present way when most children are born in hospitals under doctor's control, midwives argue for so called natural childbirth when women deliver the child surrounded by familiar environment and with midwife's assistance. The description of both groups behaviour is based on the theory of Peirre Bourdieu. Midwives are focused on the change of their status in the process of childbirth while doctors want to preserve the present situation. Both groups use different discourses while creating their professional position providing different services. Doctors' way is more technological and clinical, while midwives create their professional position in more psychological way. There is a competitive fight in which both group use strategy of their own professionalization and disprofessionalization of the rival group and different interpretation of the same data. Because of disadvantageous position of midwives in the process of childbirth, they create their own strategies against doctors.
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