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Puppies and young dogs nutritions
Šabatová, Kateřina ; Hučko, Boris (advisor) ; Plachý, Vladimír (referee)
Puppies and young dogs nutritions Summary In this work I have attempted to summarize the most important aspects in nutrition of puppies and dogs in growth. The introduction describes the digestive tract and also the changes that have occurred due to domestication. Next is explained the importance of nutrition and water in nutritional requirements of a dog. Important are needs of nutrients and energy that are necessary to maintain the provision of the necessary vital functions. The basic components of food are proteins, saccharides, lipids, minerals and vitamins. I explain the necessary proportions, the amounts and the possible consequences of their lack in food. I focus on essential fatty acids, which in recent years are subject to frequent explorations and their pros and cons are not yet fully understood. I also mention ballast and significant specific active substances. Nutrition of pregnant and lactating females is often neglected, but for puppies it is a very crucial period. The influence of nutrition of pregnant females to the size and weight of newborn puppies has been proved. Further I deal with nutrition of puppies immediately after birth, I explain the composition of colostrum and its effect on the immunity system of the puppy. I also compare ratios of nutrients in the colostrum and milk. Further, I deal with the feeding of the individual at the time of weaning and in different growth periods. There are many possibilities of feeding puppies from after weaning until adulthood described in books. With the new knowledge about nutrition the ways of feeding our dogs have inevitably changed , but also the dogs' nutritional requirements have changed due to the conditions where and how they live and what their nutrient needs are according to their sport, breeding or other use. Currently, there is a possibility of a commercially produced complete nutritional diet or home-prepared fresh food - cooked or raw- barf. In the last chapter, I summarized possible aspects of malnutrition and its potential consequences. I described the most common problems associated with inadequate nutrition of growing dogs such as obesity as well as malnutrition, which also adversely affects the individual. The influence of proper nutrition of the puppy on its growth is clear. If we neglect the nutrition of the dog in the first two years of its life, the consequences are often irreversible.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Single particle analysis of size-segregated aerosol in Prague city center
Marvanová, S. ; Skoupý, Radim ; Kulich, P. ; Bendl, J. ; Hovorka, J. ; Machala, M.
Particulate matter (PM) is omnipresent pollutant in the ambient air known to cause cardiovascular and respiratory diseases (WHO 2004). Recently, outdoor air pollution and particulate matter in outdoor air pollution were classified as carcinogenic to humans, Group 1 (IARC 2015). Especially, ambient PM of aerodynamic diameter < 100 nm, ultrafine particles, appears to be of great importance due to its high specific surface area and high number concentration (Hughes et al. 1998). Ultrafine particles also easily enter and are being transferred in organisms, and interact with cells and subcellular components (Oberdorster et al. 2005). As the evidence of ultrafine PM significance increased, size-fractionated PMs sampled by various cascade impactors have been employed into the toxicological studies on cell cultures or isolated cells, using the organic extracts of size-fractionated PMs (Topinka et al. 2013, Topinka et al. 2015) or directly the size-fractionated particles (Becker et al. 2003, Ramgolam et al. 2009, Reibman et al. 2002, Loxham et al. 2013, Jalava et al. 2006, Thomson et al. 2015, Jalava et al. 2015). The aim of this study was to evaluate shape and composition of size-segregated aerosol particles, sampled by high volume cascade impactor, using electron microscopy and energy dispersive X-ray spectroscopy (EDX).
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Formal Systems Based on Automata and Grammars
Čermák, Martin ; Rybička, Jiří (referee) ; Šaloun, Petr (referee) ; Meduna, Alexandr (advisor)
Tyto teze navazují na studium gramatických a automatových systémů. Na začátku, práce pojednává o regulárně řízených CD gramatických systémech využívající frázově strukturované gramatiky jako komponenty. Do systémů jsou zavedena tři nová omezení na derivacích a je studován jejich vliv na vyjadřovací sílu těchto systémů. Poté, tato práce definuje dva automatové protějšky ke kanonickým multi-generatiním nonterminálem a pravi\-dlově synchronizovyným gramatickým systemům, generujících vektory řetězců, a ukazuje, že všechny tyto vyšetřované systemy si jsou vzájemně ekvivalentní. Dále táto práce tyto systémy zobecňuje a zakládá fundamentalní hierarchii n-jazyků (množin n-tic řetězců). V~souvislosti se zavedenými systémy tyto teze zavádí automatově-gramatický převodník založený na konečném automatu a bezkontextové gramatice. Tento převodník je pak studovaný a použitý jako nástroj přímého překladu. V~poslední části jsou v této práci zavedené automatové systémy jádrem pársovací metody založené na stromově řízených gramatikách s n omezenými cestami.
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Test Application Methodology Based On the Identification of Testable blocks
Herrman, Tomáš ; Plíva, Zdeněk (referee) ; Racek, Stanislav (referee) ; Kotásek, Zdeněk (advisor)
The PhD thesis deals with the analysis of digital systems described on RT level. The methodology of data paths analysis is decribed, the data path controller analysis is not solved in the thesis. The methodology is built on the concept of Testable Block (TB) which allows to divide digital component to such segments which can be tested through their inputs/outputs, border registers and primary inputs/outputs are used for this purpose. As a result, lower number of registers is needed to be included into scan chain - border registers are the only ones which are scanned. The segmentation allows also to reduce the volume of test vectors, tests are generated for segments, not for the complete component. To identify TBs, two evolutionary algorithms are used, they operate on TB formal model which is also defined in the thesis.
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Methodology of highly reliable systems design
Straka, Martin ; Gramatová, Elena (referee) ; Racek, Stanislav (referee) ; Kotásek, Zdeněk (advisor)
In the thesis, a methodology alternative to existing methods of digital systems design with increased dependability implemented into FPGA is presented, new features which can be used in the implementation and testing of these systems are demonstrated. The research is based on the use of FPGA partial dynamic reconfiguration for the design of fault tolerant systems. In these applications, the partial dynamic reconfiguration can be used as a mechanism to correct the fault and recover the system after the fault occurrence. First, the general principles of diagnostics, testing and digital systems dependability are presented including a brief description of FPGA components and their architectures. Next, a survey of currently used methods and techniques used for the design and implementation of fault tolerant systems into FPGA is described, especially the methods used for fault detection and localization, their correction, together with the principles of evaluating fault tolerant systems design quality. The most important part of the thesis is seen in the description of the design methodology, implementation and testing of fault tolerant systems implemented into FPGAs which uses SRAMs as the configuration memory. First, the methodology of developing and automated checker components design for digital systems and communication protocols is presented. Then, a reference architecture of a dependable system implemented into FPGA is demonstrated including several fault tolerant architectures based on the use of partial dynamic reconfiguration as the mechanism of fault correction and the recovery from it. The principles of controlling the reconfiguration process are described together with the description of the test platform which allows to test and verify the design of fault tolerant systems based on the methodology presented in the thesis. The experimental results and the contribution of the thesis are discussed in the conclusions.
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Methodology of highly reliable systems design
Straka, Martin ; Kotásek, Zdeněk (advisor)
In the thesis, a methodology alternative to existing methods of digital systems design with increased dependability implemented into FPGA is presented, new features which can be used in the implementation and testing of these systems are demonstrated. The research is based on the use of FPGA partial dynamic reconfiguration for the design of fault tolerant systems. In these applications, the partial dynamic reconfiguration can be used as a mechanism to correct the fault and recover the system after the fault occurrence. First, the general principles of diagnostics, testing and digital systems dependability are presented including a brief description of FPGA components and their architectures. Next, a survey of currently used methods and techniques used for the design and implementation of fault tolerant systems into FPGA is described, especially the methods used for fault detection and localization, their correction, together with the principles of evaluating fault tolerant systems design quality. The most important part of the thesis is seen in the description of the design methodology, implementation and testing of fault tolerant systems implemented into FPGAs which uses SRAMs as the configuration memory. First, the methodology of developing and automated checker components design for digital systems and communication protocols is presented. Then, a reference architecture of a dependable system implemented into FPGA is demonstrated including several fault tolerant architectures based on the use of partial dynamic reconfiguration as the mechanism of fault correction and the recovery from it. The principles of controlling the reconfiguration process are described together with the description of the test platform which allows to test and verify the design of fault tolerant systems based on the methodology presented in the thesis. The experimental results and the contribution of the thesis are discussed in the conclusions.
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The Pump with a Diffuser with Stochastic Blades (Runner and Diffuser)
Dobšáková, Lenka ; Melichar, Jan (referee) ; Veselý, Jindřich (referee) ; Haluza, Miloslav (advisor)
This doctoral thesis studies the non-uniform blade cascade for centrifugal pumps. Influence of non-uniform blade cascade is investigated on the impeller and diffuser. A non-uniform blading impeller of pumps in this doctoral thesis was examined in terms of influence on efficiency characteristic. The idea of using non-uniform blading is to obtain a flat efficiency characteristic. Then the centrifugal pump could be used on larger scale of power with achieving a sufficiently high efficiency. The work is based on experimental measurements and numerical simulations of non-uniform hydraulic pump components. Non-uniform impeller and the diffuser were newly designed for this research. A new measuring track of small submersible pumps was needed to design and build for measurements. Different pump combinations with conventional hydraulics on one working point and hydraulics with non-uniform were measured. All measurements were evaluated and characteristics of efficiency, head and power were obtained for each measured combination. Some of pump combinations (which were measured in experiment) were numerically simulated by program Ansys Fluent. Data needed to evaluate the characteristic of efficiency and specific energy were collected from each numerical simulation. A theoretical consideration concerns the influence of non-uniform blade cascade applied on diffuser. The suggestion how to decrease hydraulic losses in canals was done. The theoretical consideration how to make a flat efficiency characteristic was made.
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Component method of depreciation
IMBER, Jakub
The topic of this thesis is the issue of component depreciation, which is a relatively new method of accounting depreciation. The theoretical part describes the methods of depreciation, the component depreciation method analyzed in detail and the definition of particular basic concepts in the depreciation policy. The theoretical part focuses on the issue of dismantling the component depreciation for easy understanding of the advantages and disadvantages of this method. The practical part is focused primarily on the display of depreciation in the accounts and the display of the problems on the real entity. Data from the real company are used in this section. The aim is to acquaint students with the component depreciation method and the advantages or disadvantages of using this method in practice.
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