National Repository of Grey Literature 758 records found  1 - 10nextend  jump to record: Search took 0.14 seconds. 


Fyzikálně-chemická charakterizace peptidů a bílkovin kapilárními elektromigračními metodami
Kašička, Václav ; Koval, Dušan ; Šolínová, Veronika ; Sázelová, Petra ; Prusík, Zdeněk
High-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis and electrokinetic chromatography, are presented as powerful tools for physicochemical characterization of peptides and proteins.

Characterization of plasma during organosilicone thin film deposition using haxamethyldisiloxane monomer
Blahová, Lucie ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The aim of this thesis is plasma diagnostic during deposition of thin films based on organosilicones. Hexamethyldisiloxane was used as a precursor for plasma deposition in the presence of oxygen, and the diagnosis was performed by using optical emission spectroscopy. The theoretical part summarizes the basic characteristics of plasma and processes occurred at plasma polymerization and deposition. It also deals with thin films and their use in coating materials. A relatively large part is devoted to the specification of the physical-chemical nature of the used analytical method – optical emission spectroscopy. Finally, the principles of rotational, vibrational and electron temperature calculations are described. The contemporary deposition process was carried out in continuous and pulsed mode of radiofrequently excited capacitively coupled discharge. The effects of monomer flow rate, plasma power and duty cycle on the deposition process were studied. In the individual spectra, atomic hydrogen lines of Balmer’s series as well as an atomic oxygen line were identified. Molecular bands of CO have been identified as Angstrom’s system and 3. positive system. In the case of the identified fragments, the intensity dependencies on the monomer concentration in mixture, plasma power and duty cycle were detected. Electron temperature of plasma was also calculated using the relative intensities of atomic hydrogen lines of Balmer’s series. Vibrational and rotational temperature could not have been determined because no suitable fragments for their determination were found in the spectrum. Based on findings mentioned above, partial composition of plasma and some of its properties were estimated. The subject of the further research will be determination of the exact content and structure of the thin films and investigation of other plasma characteristics. Plasma deposition is influenced by many factors, and the study of their optimal combination for the most efficient deposition process is a goal for the future research.

Structure characterization of glucanes by 13C NMR spectroscopy
Spěváček, Jiří
The principles of high resolution solid state 13C NMR spectroscopy are explained in this contribution. Some possibilities of this method in the structural characterization of glucans and their derivatives are demonstrated on examples of cellulose, chitin, chitosan and chitin/glucan complexes.

Biodegradation of environmental pollutants - Structural characterization of a new type of haloalkane LinB32 from Sphingobium japonicum UT26
NĚMEC, Pavel
The main aim of this thesis was focused on protein crystallography and attempt to crystallize model protein Thaumatin and a new prepared haloalkane dehalogenase LinB32 of Sphingobium japonicum UT26. Crystallization trials were performed using the sitting and hanging drop vapour diffusion methods, crystallization under oil and crystallization in the capillaries. After measuring of diffraction data, the structure of LinB32 was modeled by the use of visualizing molecular modeling programs WinCoot and VMD.

Underground research site Bukov - geotechnical characterization of the site
Souček, Kamil ; Vavro, Martin ; Vavro, Leona ; Staš, Lubomír ; Georgiovská, Lucie
Podzemní výzkumné pracoviště Bukov (PVP) je navrženo jako testovací lokalita pro zhodnocení vlastností a chování horninového masivu, typově blízkého vybraným kandidátním lokalitám, v hloubce odpovídající předpokládané úložné hloubce finálního hlubinného úložiště vysoce aktivních odpadů v České republice. PVP Bukov, jehož výstavba byla zahájena v roce 2013, je realizováno v jižním křídle uranového ložiska Rožná, na severovýchodním okraji strážeckého moldanubika, poblíž jeho styku se svrateckým krystalinikem. Důlní díla pracoviště jsou situována na 12. patře jámy Bukov-1, v hloubce přibližně 600m pod povrchem.
Fulltext: content.csg - Download fulltextPDF
Plný tet: UGN_0465530 - Download fulltextPDF

Příprava a charakterizace monolitických kolon na principu vtištěných polymerů
Širc, Jakub ; Bosáková, Z. ; Michálek, Jiří ; Guryča, Vilém
By in situ polymerization prepared molecularly imprinted polymer rods were characterized by porosimetry, scanning electron microscopy and by separation of standard hydrophobic solutes and enantiomer pairs.

Common attributes and specifics of urban development in GDR and the territory of the Czech Republic
Králová, Lucie ; Sládek, Jan (referee) ; Illner, Michal (advisor)
The aim of this thesis is to draw a comparison between urban development in East Germany and in the Czech Republic. While the main stress is put on characterization of the situation in East Germany, the objective of the description of Czech situation is to provide information and overview essential for comparison with the situation in GDR. Description of situation in both countries after World War II is given as a starting point, followed by detailed depiction of the socialistic era, and short characterization of urban development in 90s is given as well. The thesis is focused on overall urban development aspects such as style, form, and quality of housing, housing policies, migration, and expansion of cities. Specific factors, which influenced the urbanization in the past, and continue to have certain impact, are taken into account. Within context of social, cultural, economical, and demographical conditions, some common features may in social and spatial structure of the towns be found, but there are differences in both countries, too. Special attention is paid to individual development of both capitals Berlin and Prague. Some factors are typical for each country, playing thus specific role in urban its development. This thesis lS structured into several chapters covering introduction into problematic,...

N6-methyl-AMP-hydrolasa aktivuje N6-substituované purinové ANPs
Schinkmanová, Markéta ; Votruba, Ivan ; Holý, Antonín
Identification and characterization of a new enzyme, N6-methyl-AMP aminohydrolase, that is responsible for the conversion of N6-cyclopropyl-PMEDAP to biologically active PMEG.

Code Characterization for Automated User Interface Generation
Kadlec, Jaroslav ; Slavík,, Pavel (referee) ; Sochor, Jiří (referee) ; Zemčík, Pavel (advisor)
Práce představuje nový přístup v automatizovaném vytváření uživatelských rozhraní. Na základě taxonomie pro charakterizaci dat byla vyvinuta nová taxonomie pro charakterizaci kódu. Tato taxonomie označuje významné vlastnosti dat a kódu tak, aby ji bylo možné použít v procesu automatického vytvoření uživatelského rozhraní. Taxonomie je platformově nezávislá a může být uložena jako součást metadat nebo v externím souboru. Na základě taxonomie je představený proces automatického vytvoření uživatelského rozhraní s detailnějším popisem jednotlivých kroků. Celý proces generování uživatelského rozhraní je demonstrován na příkladech.