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Non-destructive material evaluation by non-contact ultrasound
Landa, Michal
The paper presents new non-invasive ultrasonic methods have been developed for physical properties evaluation of namely non-porrous materials. Two non-invasive ultrasonic methods (laser ultrasound, ultrasound with air-coupling) were described and advages and limitations are shown on the experimental results.
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Speculation on oil markets and its impact on commodity's price
Melcher, Ota ; Taušer, Josef (advisor) ; Baláž, Peter (referee) ; Müller, Štěpán (referee)
This study aims to analyse the precrisis period on the oil markets with a primary objective of assessing the role of speculation in the commodity's price development and its volatility. First it depicts the rapidly increasing speculative activity on the futures market together with the parallel oil price surge. The speculation is initially proxied by non-commercial traders' positions and subsequently quantified by Working's T-index. The paper then uses speculative traders' positions and both spot and futures prices to test for Granger causality within the framework of VAR models. For the sake of consistency it also evaluates causal links between speculation and inventories level. Further the study investigates the speculation impact on volatility of oil prices by employing various approaches in volatility quantification including GARCH models. Contrary to expectations we find that the speculatio's impact on both prices and their volatility is rather insignificant. In the last chapter we therefore seek for an explanation of the oil price developments by examining the market fundamentals. The interaction of supply and demand finally gives substantial evidence for understanding the price developments in the precrisis period.
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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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Cooperation between nurses and physicians from the perspective of nursing management
KADLECOVÁ, Ivana
Cooperation of nurses and physicians from the perspective of nursing management Master's thesis was focused on the cooperation of nurses and physicians from the perspective of nursing management. Nursing care and nursing management has an irreplaceable role in health care. The world of medicine, nursing care and health care is an extremely complex organizational system, which is not surely represented only by physicians and nurses, but these, however, are still the dominant persons on the scene. The first objective of this thesis was to make a survey of cooperation between nurses and physicians during the nursing process in the care of patients from the perspective of nursing management in departments of surgery and internal medicine. The second objective was to identify problem areas in cooperation between nurses and physicians from the perspective of nursing management in departments of surgery and internal medicine, and the third objective was to find out the way the nursing management tackles problem situations in cooperation between nurses and physicians in departments of surgery and internal medicine. To meet these objectives four research questions were stated: 1. In what areas do nurses and physicians cooperate during the nursing process while providing care for patients from the perspective of nursing management in their respective departments? 2. How do nurses assess and perceive cooperation with physicians from the perspective of nursing management in their respective departments? 3. Are there problem areas in cooperation of nurses and physicians from the perspective of nursing management in their respective departments? 4. How does nursing management tackle problem situations in cooperation between nurses and physicians in their respective departments? The research was conducted in the hospital, where I was given information under the condition of anonymity of the respondents interviewed. The responders were chief nurses and senior staff nurses working in departments of surgery and internal medicine. The investigation conducted is a qualitative research. Interviews with the structure of questions to non-standardized interviews were conducted with the respondents according to question structures of the non-standardized interview. Subsequently, transcripts of interviews and data categorization in tables according to the Ritchie and Spencer´s framework analysis were made. The survey suggests the following conclusions. Nurses and physicians cooperate within the nursing process in providing care for patients from the perspective of nursing management in departments of surgery and internal medicine at the stage of problem solving. Nurses appreciate cooperation with physicians on the basis of mutual respect, but they still feel the prevailing dominance of physicians. From the perspective of nursing management there are problematic areas in cooperation between nurses and physicians. Nursing management tackles problem situation in cooperation between nurses and physicians by seeking common solutions. It will be possible to provide the health care facilities, where the research was conducted, with the outcomes of the research. Thinking about the possible improvement in mutual cooperation as early as during physicians´ and nurses´ studies, to introduce various measures to improve cooperation between nurses and physicians by organizing joint seminars and cultural events could lead to meeting the purpose. It is necessary to raise physicians´ awareness of nursing process and to carry out further research on this topic.
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Vývoj metodologické a technologické platformy pro neinvazivní odhad fenolických látek v listech a bobulích
ŠEBELA, David
Plant optical signals can serve as important source of information about biochemical and physiological processes in plants. These signals are influenced by compounds synthesized by plants during primary or secondary metabolism and thus, can also serve as their qualitative and quantitative indicators. Light reaching plant surface (leaf or fruit) can undergo three main pathways- it can be (i) reflected, (ii) absorbed or it can (iii) transmit through plant material. The probability of these three processes depends on particular wavelength of incident irradiation and on the morphological characteristics of plant tissues themselves. As such, plant contains various spectrum of photosynthetic pigments and fluorescent compounds which can either reflect, absorb or pass incident irradiation through at specific wavelengths. Biophysical techniques working with these optical properties of plant pigments and/or other compounds have become universal and common tool in basic and applied research. To quote some example, chlorophyll fluorescence imaging, UV induced fluorescence or spectroscopic techniques are on the top of interest thanks to its non-invasive nature, allowing maintain the integrity of measured cells or the whole plant constituents. The main aim of this thesis is to provide a comprehensive study on the possibility of non-invasive monitoring of phenolic compounds in the leaves and fruits.
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Injuries of children at home
POCHYLÁ, Petra
The theoretical part of this thesis is oriented at an actual issue of accidents involving children in home environment, their prevention and a correct provision of first aid by a non-professional at these types of selected accidents. The practical part of this thesis was led by a qualitative form of research, a method of a semicontrolled interview. The interview given was led by chosen caring people, who gave first aid to children with a home environment injury. The whole process of the interview was recorded at a digital record book and then rewritten to a text form, with the approval of the respondents. The respondents were chosen on the basis of a survey. The final information from the interviews were analyzed and subsequently summarized into results. Six respondents were registered into the research part. Six basic questions were given to the respondents. The questions were chosen to find out information about an injured child, an injury character, a procedure of caring person while giving first aid, or other professional care and permanent effects. While preparing the research part, I was expecting that most of the respondents would manage to give first aid, however, according to the results, half of the parents didn't know how to deal with giving first aid. Non- professional first aid, provided at the right time and done correctly, is one of the basic and essential steps to reverse the injured person back into the normal quality of life. This is why, all people should be able to give first aid without special equipment and tools. The aim of my thesis was to monitor the process of the caring person while giving first aid for selected accidents involving children in home environment. I gave myself one fundamental research question: What was the process of the caring person while giving first aid for accidents involving children in home environment? At the final evaluation and comparison of my results, I achieved the results, in most examined facts, which are presented in other different studies dealing with similar issues- children's injuries and giving first aid for accidents involving children.
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THE DRAMATICAL SCRIPT AS A NECESSARY BASIS FOR SPACIAL REALIZATOIN
Tretiag, Štěpán ; KORČÁK, Jakub (advisor) ; HRBEK, Daniel (referee)
Using the concrete examples of my practical bachelor work my intent is to retrospectively describe and reflect on our scenic realization of the play Missing by south-African author Reza de Wet. The essence of a modern dramatic direction is to perceive the script of a play as a model which becomes a basis for its interpretation and the subsequent staging of the play. It is the process from the initial interpretation to final scenic realization I wish to focus on in my work.In the first part I will be dealing with creation of a physical space, that being stage design. I will try to capture what lead us to its final shape and how the basis for that can be found in the script of the play as well as the potential capability of the physical space to become a dramatic space. Its ability to create a platform for the actors to proceed from and how can it provoke them into acting, in particular. In the second part I will be dealing with the dramatic space, ergo the spacial expression of relationships and themes. I will proceed again retrospectively, focusing on how we managed to achieve the stage image which is functioning as a materialized metaphor. I will focus mostly on non-verbal actions, but with regard to the fact, that dramatical script is the basis for our realization of the play.
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Estrogen receptor beta (ERβ) in testicular cells and sperm
Dostálová, Pavla ; Žatecká, Eva ; Děd, Lukáš ; Dorosh, Andriy ; Postlerová, Pavla ; Jonáková, Věra ; Dvořáková-Hortová, Kateřina ; Pěknicová, Jana
Estrogen is a steroid hormone that plays an important role during sperm development in the male and female reproductive tract. Estrogen signalling is a complex process that depends on cell milieu and presence of receptors. Thanks to the steroid nature of estrogens, they can pass through the plasmatic membrane and bind to the intracellular estrogen receptors (ERs). Within the cell, there are several pools of ERs. One of them is localized to the cell nucleus and their activation leads to direct or indirect binding to DNA and ultimately to alternation in gene expression (genomic pathway). Other pools of ERs are associated with plasma membrane or are located in cytosol. Activation of membrane associated ERs leads to rapid non-genomic responses. Nowadays, two classical estrogen receptors are known – ERα and ERβ. Since ERβ is a predominant variant in testes, we focused our study on expression of ERβ variants in murine testes and sperm. We detected two variants of ERβ at mRNA level in both, testes and sperm. These variants differ in 54 nucleotids within the ligand binding domain and this variability results in different affinity to estrogen. We analyzed individual testicular cell types (spermatogonia, spermatocytes, spermatids, Sertoli cells) by RT-qPCR. Our results suggest that both ERβ variants are coexpressed in the same cell type and may therefore interact together. This may have consequences in mediating of estrogen signalling. Moreover, ERβ is expressed more in the later stages of spermatogenesis suggesting the role of ERβ in these stages or alternatively in spermatozoa alone. At the protein level, we detected ERβ in nuclear, membrane and cytosolic fraction prepared from testicular tissue suggesting the involvement of both, genomic and non-genomic, pathways of estrogen signaling in testes. In sperm, anti-ERβ antibodies localized ERβ in acrosome region and tail which is in accordance with the known role of estrogen on capacitation, acrosome reaction and motility.
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Experiences of health workers with acupressure
ROUBÍČKOVÁ, Radka
The abstract of the diploma thesis is focused on the acupressure and its influence of backache of medical staff. We chose the theme of acupressure because it is a non-invasive treatment method which can be practiced by everyone in the comfort of the home and no aid is necessary to have, just the basic knowledge and training how to perform acupressure. Acupressure is not physically or time consuming and that is why it is considered as a suitable means as an additional treatment of pain and other health disorders. It can improve nursing care. The theoretical part deals with pain and pain of medical staff who are the highest risk group of backache in the lumbar region. The backache is the most frequent cause of incapacity for work. Furthermore, in the theoretical part we deal with acupressure, one of the methods of alternative medicine. For the processing of the theoretical part, we used the available foreign and Czech literary sources. The empirical part of the diploma thesis is a quantitative method. The results obtained from the research were processed in the overview tables and case reports. For the thesis we set two research objectives. The first objective was to find out the experience of medical staff with acupressure. The second objective was to verify the influence of the techniques of acupressure for backache of medical staff. On the basis of the aim two hypotheses were created. The first hypothesis should show whether the backache will be softened by application of the acupressure techniques. The second one shows whether there is the difference in approach of nurses and doctors to perform acupressure.
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