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název v anglickém jazyce není uveden
Kolesová, Hana ; Grim, Miloš (advisor) ; Slípka, Jaroslav (referee) ; Nečas, Emanuel (referee)
Branchial arches region and its blood vessels are extensively transformed in the embryonic development. Aim of this study is to investigate mechanisms of the branchial arches region development and to study how a morphogen Sonic hed gehog (Shh) participate on the formation and remodeling of branchial arches and their blood vessels. Influence of Shh was evaluated based on the changes caused by its inhibition in vivo. Shh function was inhibited with an anti-Shh antibody, which was produced into the embryo from the applied hybridoma cells. Shh signaling cascade was also inhibited by cyclopamine. Results show that Shh is important for dc novo format,ion of the blood vessels in the branchial arches region. Further Shh is necessary for stabilization of the vessel wall, mainly for anterior cardinal vein. Shh also alfects later vessel development and transformation, which includes i.e. fusion of the dorsal aorta, branching of the internal carotid artery and outflow tract development. Short time inhibition of Shh has minor effect on the apoptosis and proliferation activity of the branchial arches region mesenchymal cells. We assume that Shh signals directly to the blood vessels endothelial cells, as Shh receptor ptel is also expressed in endothelial cells and its signal is reduced with Shh inhibition. In studied...
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Biomarkers in Myocardial Damage Diagnostics
KOPŘIVOVÁ, Olga
Anotace (abstract) Biomarkers diagnosing myocardial damages have recently been a very dynamically developing part of clinical biochemistry. Implementing of new biomarkers is very prompt; they are mostly implemented into practice without wider clinical and analytical experience. During a few years these are new methods to determine myoglobin, troponins (cTnI, cTnT), CK-MB mass, BNP etc. The effort of clinical biochemists and cardiologists is to cover all phases and levels of myocardial damage in acute coronary syndromes by means of cardio markers. These investigations are dramatically changing the view of diagnosis, determination of prognosis and therapy in acute coronary syndromes. In my thesis I covered retrospective data analysis in patients with the pain in the chest, presented at an inpatient clinic. The value of myocardial damage indicators and their dynamism was analysed from the definitive diagnosis point of view. I performed my own analysis at OKB Nemocnice Písek, a.s. (Hospital Písek, Inc.). The aim of my thesis was to cover the significance of particular myocardial damage markers and find out if investigated set of markers is sufficient for correct and prompt diagnosis of acute myocardial infarction. In terms of this thesis I tried to find out if the panel of cardiac markers contains excess values. In OKB Písek, a.s. the panel of myocardial damage markers includes: myoglobin, troponin I, CK-MB mass and the marker of heart failure BNP. In the samples of fifty-six patients I analysed the presence of the above mentioned markers of myocardial damage and heart failure. I examined only the markers which were determined by a physician. In the group of investigated patients with chest pain, measured values of troponins proved myocardial infarction in sixteen patients, values of CK-MB mass in sixteen patients and values of myoglobin in twelve patients. In the control group of twenty patients with chest pain the boundary values of troponin I were measured in two patients, without the increase of other myocardial damage markers. High value of troponin I was measured in two patients in non-ischemic myocardial damage (acute pericarditis), which agrees with data in scientific literature. The thesis shows that the level of troponin I reflects myocardial damage relatively accurately; troponin I is sufficiently cardio specific but is not a sufficiently early marker. For early management of acute myocardial infection it is important to determine myoglobin, which is detectable within two hours since the beginning of troubles but is not sufficiently cardio specific. The course of CK-MB mass elevation wave copies the course of troponin I elevation wave. That is why the investigation of this marker appears to be excessivet at current investigation of troponin I and myoglobin to prove myocardial damage, which is in agreement with scientific association recommendations.
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The endocrine function of adipose tissue and its importance for initiation and development of insulin resistance and diabetes
Haluzík, Michal ; Schreiber, Vratislav (advisor) ; Marešová, Dana (referee) ; Šeda, Ondřej (referee)
Endocrine production of adipose tissue is a very complex process affected by numerous endogenous and exogenous stimuli. Peroxisome proliferator-activated receptors-alpha (PPAR-) are important modulators of metabolic processes which can also affect endocrine function of adipose tissue. Recently, numerous novel factors produced by adipose tissue with important metabolic effects were identified. Some of them can directly bind PPAR receptors. One of the examples of these factors is fatty acid binding protein 4 (FABP4) which can directly bind PPAR receptors and indirectly modify its activation by changing availability of endogenous PPAR ligands -free fatty acids. We hypothesized that the mechanism of action of PPAR receptors to metabolic processes may partially lie in their complex interaction with adipose tissue-derived hormones. The unraveling of these interactions may have important consequences in finding approaches to treat patients with type 2 diabetes mellitus. (...) In summary, our data show an important role for the interplay of PPAR activation and endocrine function of adipose tissue in metabolic regulations which may have important clinical consequences.
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Ectopic pregnancy - etiology, modern diagnostic and therapeutic approach
Fischerová, Michaela ; Drahoňovský, Jan (advisor)
The goal of this work entitled Ectopic pregnancy - etiology, modern diagnostic and therapeutic approach, was to give a complete and complex summary of this topic. Ectopic pregnancy is the most frequently occurring acute abdomen episode in the Czech Republic and it is an important factor of female morbidity and mortality, therefore it deserves special attention. The paper clearly states the risk factors of ectopic pregnancy together with methods and recommendations for the prevention and therapy of the risk factors. The following part of the text gives the reader a closer look on the different possible clinical forms of ectopic pregnancy. Further, the possibilities of modern diagnosis are analyzed, with a major importance given to the use of ultrasound imaging. The end of the work is given to today's therapeutic possibilities. The condition as a whole, as well as specific situations are described.
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Narcolepsy and other seizure disorders
TONDLOVÁ, Andrea
The thesis deals with the issue of seizure disorders from the perspective of special neurology. In terms of content, it is focuses on two major areas. The first part is theoretical in nature and has descriptions of the individual disorders, their clinical manifestations, diagnoses and current treatments. It is focused on the following seizure disorder group with regard to one of the objectives of the work: epilepsy, tetany, Meniere's syndrome, migraine and more specifically narcolepsy. These findings are supplemented with information on current knowledge regarding the treatment and diagnoses of these diseases, which make the lives of thus disables people very difficult and prevent them from leading their lives the way they could if they were in full health. The aim of the work is not just a theoretical description of the currently available knowledge of these diseases from literature, but also a practical confrontation thereof from experiences, which in this case result from the true stories of people suffering from seizure disorders. A specific seizure disorder has been selected for this second part of my thesis, which needs to be talked about more in order to become better known. It is a disorder that is interchangeable with regard to its symptoms, which are similar to other disorders, and it is difficult to diagnose or is only diagnosed after an inconveniently long time - i.e. Narcolepsy . The thesis therefore deals with the issue of narcolepsy in most detail, with regard to the practical focus in the next section, where in addition to aforementioned basic knowledge, the author describes the activities and the importance of sleep laboratories and centres as institutions involved in treatment. In order to penetrate into the issue of this disorder even more deeply, she adds the aforementioned anonymous stories of people affected in this way. This part is also complemented with illustrative images of the sleep laboratory and the stories of these people as a better visual complement of the thesis for the reader. A descriptive analysis (comparison) of each symptom was chosen as a comparative method of the aforementioned thesis objective (to compare the theoretically given symptoms of narcolepsy with the symptoms of individuals suffering from this disease), in which the goal was to choose the common symptoms these people which make their lives difficult and compare these data with information (symptoms of narcolepsy) found in contemporary literature. In conclusion, we visit the most important information from scientific literature, the views of the professional community on the current research and studies on narcolepsy, i.e. the specifically examined causal links of applying the Pandemrix vaccine (against swine flu) and narcolepsy and a summary of the results of other studies investigating the etiology of this disease. The aim of this section is to present the reader with, and identify, the current developments in narcolepsy research. An evaluation of the historical development of interest in sleep disorders and an evaluation of the conclusions drawn by comparing symptoms specified in professional literature on narcolepsy with actual symptoms of people suffering from this disease are also included. The aim is to provide the reader with a bio-psycho-social view of a person with this disease, which is not always emphatically discussed in every piece of professional literature. The result of the entire thesis is a summary of the current state of knowledge of these diseases and a reflection on the research of the current possible causes of narcolepsy. It continues to compare the theoretical background of the narcolepsy disorder with the experience of living with this disease, including stories of selectively chosen individuals suffering from narcolepsy.
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Education of the diabetics in the origin of possible complications related to diabetes mellitus
Krejčová, Monika ; Schneiderová, Michaela (advisor) ; Knížková, Gabriela (referee) ; Vachková, Eva (referee)
This disertation is concerned to the education of the diabetics about the origin of possible complications that appear in relation with diabetes mellitus. Theoretic passage is oriented to the education which is a necessary component of treatment, to its importance, to goals, phases, form, means of the education, organisation and contents. This passage also approximates the issues of diabetes mellitus complications, such as classification, causation, pathogenesis, clinical picture and therapy of particular complications. The core of this work has been created from a quantitative survey about the knowledge of diabetes mellitus complications, and was made from the diabetics and the common nurses at Regional Náchod Hospital in Broumov. The resultant knowledge should be similar due to educational activities of the nurses. The outcome of this work is a suggestion of the educational plan for diabetics which is oriented to prevention from emergence of diabetic foot.
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Prognostic importance of angiopoietin-2 gene transcription in chronic lymphocytic leukemia.
Gradošová, Iveta ; Pecka, Miroslav (advisor) ; Smolej, Lukáš (referee)
Chronic lymphocytic leukemia (CCL) is the most common leukemia, which is characterised by a high variable progress. According to scientific evidence acquired during several last years, angiogenesis has been proven to play an important role in pathogenesis of CLL. One of the very important cytokines playing role in this process is angiopoietin-2 (Ang-2). Elevated expression of Ang-2 has been reported in several hematological malignancies; however, data regarding Ang-2 in CLL are very limited. I measured Ang-2 transcript levels in purified mononuclear cels of 24 untreated patients with CLL by quantitative real-time PCR and normalized them for differences in RNA concentration in each sample by quantitation of transcript of Abl1 housekeeping gene. Elevated Ang-2 mRNA concentrations were detected in 10 cases (Ang-2 to Abl1 expression ratio > 5x10-3). On the other hand, 14 patients had very low or undetectable levels of Ang-2 mRNA (Ang-2 to Abl1 expression ratio < 5x10-3 or Ang-2 Cp > 35). The association between Ang-2 expression and other prognostic factors, clinical stage according to Rai and clinical course of CLL was evaluated by Chi-Square method. There was a significant association between high Ang-2 mRNA levels and unmutated IgVH genes (n= 21, p = 0.012), but not with CD38 (p = 0.057), ZAP-70 expression (p...
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