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Třeboňsko 2000. Ecology and Economy of the Třeboň Region after 20 years
Pokorný, Jan ; Šulcová, J. ; Hátle, M. ; Hlásek, J.
The conference Třeboňsko 2000, with its sub-title The ecology and economy of the Třeboň Region is the third of such conferences - following two ones, held in 1978 and 1988, respectively. The present conference has continued in the fine tradition of good communication between all the subject areas thet are inevitably involved in, and/or influenced by, the conservation and development of the Třeboň Basin Protected Landscape Area and Biosphere Reserve
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Cultivation of grapevine in conditions in vitro
Faltus, Miloš ; Skala, Ondřej ; Bilavčík, Alois ; Zámečník, Jiří
Grapevine is a traditional crop in the Czech Republic, especially in the South Moravia region. It is a vegetatively propagated crop; hence vineyards are endangered by abiotic and biotic stress influence. The risk of plant damage due to frost and pest influence occurs in the field conditions according to season and locality. Cultivation of grapevine in conditions in vitro eliminates the risk. Higher utilization of the method is limited by sensitivity of some genotype to in vitro conditions. This methodology solves these problems and describes material introduction into in vitro conditions, explants multiplication and slow growth condition establishment. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
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International conventions about protection of nature and landscape
KUDRLIČKOVÁ, Adéla
Content of this work is focused on international conventions about protection of nature and landscape. This topic is elaborated in the form of literary research, which analyses nature and landscape conservation in the Czech Republic region and from the viewpoint of international cooperation of the Czech Republic and its memberships in international organizations as well.
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Thin discs and rings as sources of Weyl space-times
Kubíček, Jan ; Semerák, Oldřich (advisor) ; Žofka, Martin (referee)
Static and axially symmetric vacuum solutions of Einstein's equations can be descri- bed by the Weyl metric which only depends on two unknown functions, given by the Laplace equation and a line integral. In this thesis we study some properties of two Weyl space-times whose sources are one-dimensional rings - the Appell ring and the Bach-Weyl ring. On the behaviour of proper distances and geodesics in the central region we demonstrate that in Weyl coordinates these sources represent directional singularities. 1
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Interactions of Trypanosoma brucei FOF1 ATP Synthase Subunits - An Application of Yeast Two Hybrid
GUGGENBERGER, Matthias
The FOF1 -ATP synthase has a unique composition and function in Trypanosoma brucei, a flagellated parasite endemic to developing regions of the world where it causes Human African Trypanosomiasis (HAT) and Nangana in cattle. Currently there are no good treatments to cure the disease as most of the medicine is antiquated, difficult to administer and highly toxic. Because of the unique properties of the T. brucei FOF1-ATP synthase, this enzyme is considered a possible drug target. This final component of the oxidative phosphorylation pathway actually works in reverse during the infectious stage of this parasite as it needs to hydrolyze ATP to maintain the essential mitochondrial membrane potential in the absence of a cytochrome mediated respiratory chain. In addition to the well conserved eukaryotic subunits, this large complex is also comprised of several subunits that have no known homology outside of kinetoplastids, an ancient group of protists with a unique mitochondrial DNA structure. To further explore the composition and organization of this potential drug target, we employed the technique of yeast two-hybrid to map the protein-protein interactions of the individual subunits. Our preliminary results suggest that important information about the organization of the T. brucei FOF1-ATP synthase can be gleaned from this experimental approach.
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není uveden
Bahannan, Abdulrehman ; Slavíček, Aleš (advisor) ; Dlouhá, Olga (referee) ; Slípka, Jaroslav (referee)
Endoscopic laser-assisted cordectomies classified by Remacle and Rudert (typ I-V cordectomies) comprise a vast range of procedures from simple vocal cord stripping or submucosal cordectomy (type I) through more extensive surgery (subligamentous cordectomy- type II, transmuscular chordectomy- type III) to complete (type IV) or extended cordectomies (type V) used when tumour involves the anterior commisure, arytenoid region, subglottic region etc. Endoscopic laser-assisted surgery of the larynx is usually indicated in early stages of infiltrative malignant disease of the glottic region (Tla-b, T2) or in preneoplastic conditions (laryngeal intraepithelial neoplasia - LIN I, II or III). Radiotherapy is also believed to be an equivalent type of oncological therapy for these diseases. Both surgery and radiotherapy can worsen one of the main functions of the human larynx- the human voice. Because the overall survival rates and local control rates are quite similar in both types of therapy, one should always have in mind something that is beyond the main goal of achieving radical removal of the tumour- patients quality of life (QoL) and quality of voice. It is very interesting to compare QoL and voice quality in different types of treatment. The possibility to conserve the radiotherapy for the possible recurrence...
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Structural flexibility of regulatory DNA element
Řezáčová, Barbora ; Štěpánek, Josef (advisor) ; Rosenberg, Ivan (referee)
Transcription of the genetic code is controlled by numerous proteins. Some of them, so-called transcription factors, bind to certain DNA regions possessing anomalous properties due to their specific and highly conservative base sequence. One of these DNA segments is SRE (Serum Response Element). The diploma work is linked to results of the previous study indicating that the structural flexibility of this segment, related to the CArG box sequence, plays a key role in its physiological activity. Our work is devoted to study of this DNA segment and its model sequential variants. The approach of accurate measurements of temperature dependent UV absorption spectra and their consecutive analysis by means of factor analysis techniques and difference spectra construction, the main part of which was also developed within the framework of this work, was employed. The results indicate that separate chains of SRE with various length form surprisingly stable hairpins, which in some cases prevail even in mixtures of both chains of the SRE segment over duplexes. By using a fitting to thermodynamic equilibrium equations, thermodynamic characteristics of temperature induced association and dissociation transitions of complexes, both intermolecular (duplexes) and intramolecular (hairpins), were obtained.
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Possibilities of Physiotherapy for chronical overload of the lumbar spine of female floorball players
SLADKÁ, Zuzana
The Bachelor's work deals with the topic of women's floorball from the physiotherapeutic point of view. Due to the basic playing posture in a continuous forward bend and the one-sided holding of the floorball stick, the lower back is being overloaded in long term. Stabilization of this region is secured by the deep stabilization system that works based on the interaction of the spine extensors and flexors. Is the activity not balanced, muscle disbalance and micro trauma occur, which have a negative impact on the player performance. Including of compensation and relaxation exercises into the training programme may work as prevention of development of such musculoskeletal overload. The aim of this work is to present the problems of women's floorball from the physiotherapeutic point of view and to offer possibilities of particular kinesiological and physiotherapeutic methods for prevention of injuries and spinal overload. The theoretical part describes the functional anatomy, the biomechanics of the lower back and the deep stabilization system assuring the stability of this spine region. Furthermore, the issues of floorball, the basic playing activities of individuals with the description of the floorball player posture are described. The theory quotes examination methods and therapy as well. This fulfils the first aim of the bachelor's work regarding closer description of the women's floorball issues. The research part of the work is processed by the quality research method. For analysis, the case interpretation of three floorball players of the FBC Liberec extra league team has been used. These three players had problems in the lower back region, mostly accompanied by pain. Every case interpretation involves input and output medical inspection with anamnesis definition, static inspection of aspection and palpation in the pelvis region, goniometric and somatometric inspection with function muscle test, dynamic inspection (Trendelenburg-Duchenne test), inspection of movement stereotypes, postural stabilization, hypermobility and shortening of muscle groups. Within this therapy, soft tissue techniques, stretching, method of the dynamic neuromuscular stabilization, parts of the Ludmila Mojžíšová method, sensomotoric stimulation and examination on a big gymnastic ball, have been used. Based on an assessment, both, the short-term and long-term individual kinesiotherapeutical plan has been developed and the second aim of the bachelor's work fulfilled show the possibility of particular kinesiology and physiotherapy methods for prevention of injuries and spinal overload. The therapy results are positive and the selected physiotherapeutical methods have proved to be effective. The posture in the lower back region has improved with all players and also symetrization of the trunk as well as reduction of pains as a result of the deep stabilization system could be observed. Both of the parties have evaluated the effect of the therapy as positive. The bachelor's work can be used in the physiotherapeutical clinic practice and as a resource for increasing the awereness of the sportspeople and trainers.
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View on the Greek Traditional Music
Valai, Konstantina ; Stříteská, Leona (advisor) ; Nedělka, Michal (referee)
97 Summary This diploma dissertation is concentrated in Greek traditional music and traditions. Greece, is a country with traditions and customs alive even today. Through dance, song, music people express their feelings, celebrate the joy and settles with the pain. It's a place where history, religion and music are closely tied together and are often difficult to distinguish. This knowledge will ultimately help all teachers understand the power of tradition and the importance of conservation and disseminate it. All are interrelated and the music is the force that unites them and is keeping them over the time. The first chapters focus on theoretical approach and the second part to the practice. Specifically, in the first chapter begins with a definition, as much as possible, of music and a small reference to it, in time. The second chapter deals with the folk song, how is divided and the differences with the European music. Into the third chapter we meet the Greek traditional music instruments and in which categories it divides. How the culture is connected with history and Orthodox Church we will see in fourth chapter as well as the most important celebrations of Greek nation. The dance in Greece has big history and we can find a lot of styles depending of the regions. Six big regions have Greece and we...
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The role of conserved residues in the S4/S4-S5 region of vanilloid TRP channels
Boukalová, Štěpána ; Vlachová, Viktorie (advisor) ; Doležal, Vladimír (referee)
The vanilloid transient receptor potential channel TRPV1 is a tetrameric six-transmembrane segment (S1-S6) channel that can be synergistically activated by various proalgesic agents such as capsaicin, protons, heat or highly depolarizing voltages. In TRPV1 channel, the charged residues in the S4 region and intracellular S4-S5 linker have been proposed to constitute a part of a voltage sensor that acts in concert with other stimuli to regulate channel activation. Molecular basis of this gating event are poorly understood. We mutated charged residues all along the S4 and the S4-S5 linker of TRPV1 and related vanilloid receptors to identify potential voltage-sensing residues. The functionality of most of the TRPV1 mutants was altered with respect to voltage, capsaicin, heat and/or their interactions. We identified two amino acid residues (R557 and D576) that could potentially constitute part of TRPV1 voltage sensor. The non-functional charge-reversing mutations R557E and R579E were partially rescued by charge-swapping mutations R557E/E570R and D576R/R579E, indicating that electrostatic interactions contribute to allosteric coupling between the voltage-, temperature- and capsaicin-dependent activation mechanisms. The mutant K571E was normal in all aspects of TRPV1 activation except for 2aminoethoxydiphenyl...
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