|
Determine the value of the company
NOVÁKOVÁ, Petra
This work deals with the value assessment of a given company for booking period from 2008 to 2012. Yield methods were used for the company evaluation, the method based on EVA and discounted cash flow method (DCF) with exploitation for both the owners and the creditors. In conclusion of this work the overall results achieved were compared.
|
|
Determination of the company value
TALÍŘOVÁ, Klára
This diploma thesis was focused on determination of the company value in the chosen company. For this purpose was chosen two mostly used methods on the Czech market. First one was method of discounted cash flow and the second one was method of the economic added value. Main sources for all the calculation were annual reports and financial statements for the period of five last years from 2011 to 2015. These basis were used for developing a prediction for the following five year period till 2020.
|
|
Comparison of HPLC a GC-MS method for pesticide determination in soil
Chalbia Václavíková, Alena ; Kočárek, Martin (advisor) ; Havlík, Jaroslav (referee)
Physical and chemical properties of pesticides may vary significantly. Pesticides can be acidic, neutral or alkaline. Some compounds containing phosphorus, other sulfur, nitrogen or halogens. These atoms can be important for the detection of pesticides. Some of the compounds are volatile, others on the contrary not evaporate, which is also one of the important properties for chromatography. The diversity of pesticides actually makes it impossible to create a universal method, which is very impractical in terms of quality and time determination. Because that pesticides are one of the most chemicals in most countries. In the food and drinking water are limits on pesticides in order to avoid negative impact on public health. For residues in soil or surface water limits are set. (Lutz et al., 2006)
The objective of this thesis is to evaluate the possibility of using two types of chromatographs with different detectors for the analysis of pesticide. Pendimethalin which was chosen for this work, will be analyzed usinga liquid chromatograph with UV detektor and gas chromatography with mass detector. Both this method will be compared as well two extraction methods (Soxhlet extraction and shaking), and two extraction agents (methanol and acetonitrile).
Another point of the work is to provethe hypothesis that, for the determination of pendimethalin in soil is by gas chromatography using a mass spectrometer in comparison with the high performance liquid chromatography time and more expensive, but will achieve a higher yield and a lower detection limit of the pesticide.
|
|
Impact of pesticides on the gut microbiota of animals
Stehlík, Michal ; Rada, Vojtěch (advisor) ; Salmonová, Hana (referee)
Pesticides are substances used to control plant and animal pest in agriculture. They are very significant and important factor in both production and revenues. Nowadays it is almost impossible for farmers to dispense without these substances but their presence in the soil does not bring only benefits. About pesticides it is generally known that they may adversely affect either on the ecosystem or the human or animal body. As the result many substances had been banned from market. Pesticides and their residues are subject to many controls and laboratory analysis in order to prevent their negative effects.
The aim of the thesis was to test the effect of pesticide (Roundup) on gut microorganisms. For the experiments we used bovine fecal samples and pure bacterial cultures isolated from various domestic animals for the testing of susceptibility to Roundup. We have determined specific growth rates of pure cultures cultivated on three different concentrations of Roundup and total counts lactobacilli, enterococci, E. coli and coliform bacteria of bovine feces cultivated on six different concentrations of Roundup
In the first experiment, no correlation has been found between the concentration of glyphosate (active substance of Roundup) and counts of bovine intestinal bacteria. The effect of glyphosate on the growth of intestinal bacteria was visible at the second attempt. At the highest concentration of glyphosate 17 g/L. Growth curves reached considerably lower levels here than at lower concentrations of glyphosate. Control samples and the samples on the concentration of glyphosate 1.7 mg/L and 0, 17mg/L increased similarly in most cases. This implies that in these concentrations there was no inhibition of bacteria whatsoever. The least susceptible genera to glyphosate were lactobacilli. The highest susceptibility to glyphosate was detected in bovine bacteria and the lowest in chicken bacteria.
|
|
Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains.
In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05).
In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter).
The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined.
Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb).
Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.
|
|
Suitable Methods of Determination and Varification Ammonia and Selected Greenhouse Gasses Detection
Zabloudilová, Petra ; Pecen, Josef (advisor) ; Jan, Jan (referee)
The dissertation deals with methods of detection gases from agriculture, especially ammonia. To understand and control of ammonia and others gases (i.g. methane) depends on sampling and measurement techniques that includes devices, instruments and procedures. In the theoretical part are listed the basic characteristics of ammonia and selected greenhouse gases and its environmental effects, sources of emissions and characteristics of chosen gas sensors (especially semiconductor sensors). In the experimental part of the work were tested conditions of chosen types of sensors (especially semiconductor sensors) mentioned above in the laboratory, according to the change of relative humidity and concentration detection gas especially. The chosen sensors were verified for measuring in the operational conditions (in the barns). Finally, the advantages and disadvantages of tested sensors are discussed (with regard to use these sensors in operational conditions).
|
|
Assessment of in vitro antioxidant properties of medicinal and edible plant extracts
Tauchen, Jan ; Kokoška, Ladislav (advisor) ; Jaromír , Jaromír (referee)
Identification and characterization of plant-based products with antioxidant and anti-proliferative effects has received much interest over the past few years as possible therapeutic mean for treatment of diseases likely to be associated to oxidative stress (such as atherosclerosis, diabetes, and cancer). This study provides characterization of in vitro antioxidant and/or anti-proliferative potential and phytochemical profile of (i) 39 wine samples of underutilized Georgian grapevine cultivars, (ii) extracts of 22 samples of medicinal plants from Ethiopia and (iii) 23 samples of edible and medicinal plants from Peruvian Amazon. For this purpose, 2,2-diphenyl-1-picrylhydrazyl radical assay (DPPH), oxygen radical absorbance capacity (ORAC) assay, total phenolic content (TPC), and cell viability assay based on metabolization of tetrazolium bromide (MTT) to formazan, together with methods based on high performance liquid chromatography (HPLC-ultra violet/visible spectrometry and HPLC-tandem mass spectrometry) were used. Georgian red wines (particularly Saperavi cultivars) exhibited higher antioxidant capacity (average DPPH and ORAC values at 5.1 and = 10.6 g TE/L wine, respectively) in comparison to Central and West European cultivars [Pinot Noir (DPPH = 3.1 and ORAC = 9.4 g TE/L wine), Cabernet Sauvignon (DPPH = 3.0 and ORAC = 7.3 g TE/L wine) and Cabernet Moravia (DPPH = 2.0 and ORAC = 8.5 g TE/L wine)]. Georgian wines contained significantly greater concentrations of quercetin (between 14.44 and 1.07 ug/mL), kaempferol (between 1.68 and 0.03 ug/mL) and syringic acid (between 12.59 and 4.72 ug/mL), whereas possessed lower quantities of resveratrol (between 5.11 and 0.32 ug/mL) in comparison to Central and West European wines. Amongst edible and medicinal plants from Ethiopia and Peruvian Amazon, only Dodonaea angustifolia (IC50 for DPPH = 22.2 ug/mL, ORAC = 767.6 ug TE/mg extract; IC50 for Hep-G2 = 120.0 ug/mL), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract; IC50 for Hep-G2 = 50.5 ug/mL), Inga edulis (DPPH and ORAC = 337.0 and 795.7 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 36.3 and 57.9 ug/mL) and Oenocarpus bataua (DPPH and ORAC = 903.8 and 1024.4 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 102.6 and 38.8 ug/mL) have demonstrated combinatory antioxidant/anti-proliferative efficacy. Selective anti-proliferative activity was observed for Verbascum sinaiticum (IC50 for Hep-G2 = 80.6 ug/mL) and Annona montana (IC50 for Hep-G2 and HT-29 = 2.7 and 9.0 ug/mL, respectively). Above-mentioned plant material showed only weak or non-toxic effects towards normal cell line. Despite the fact that extracts of Jasminum abyssinicum (IC50 for DPPH = 26.3 ug/mL, ORAC = 1023.7 ug TE/mg extract), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract), Mauritia flexuosa (DPPH and ORAC = 1062.9 and 645.9 ug TE/mg extract), Myrciaria dubia (DPPH and ORAC = 641.9 and 642.6 ug TE/mg extract) and Theobroma grandiflorum (DPPH and ORAC = 714.8 and 821.9 ug TE/mg extract) have exhibited considerable antioxidant effect, these species were found to possess moderate to low anti-proliferative potential or have shown to be toxic to normal cells line. In all cases it was detected that phenolic compounds content correlated strongly with antioxidant activity, however weakly with anti-proliferative effect. Results suggest above-mentioned species as prospective materials for further development of novel plant-based agents effective against oxidative stress related diseases. However, it is necessary to perform further research which would be focused on detailed characterization of their chemical composition, pharmacological effects and toxicological safety, in order to verify their possible practical use.
|
|
The Evaluation of Reproduction in Bactrian Camels (Camelus bactrianus) and the Possibilities of Using Non-invasive Methods for Detection of Heat and Pregnancy
Fedorova, Tamara ; Lukešová, Daniela (advisor) ; Jiří, Jiří (referee)
Camels are important husbandry animals which are also often bred in zoological gardens. Unfortunately, camels in European zoos are not usually trained and pregnancy diagnosis in a half-tamed camel is very difficult. Moreover, information of the maternal behaviour of camels is limited. This thesis reviewed current knowledge on camel husbandry, reproduction and behaviour and aimed to 1) examine non-invasive methods of heat and pregnancy diagnosis from urine and saliva in camels kept in zoological gardens; 2) explore their maternal and suckling behaviour; 3) describe experiences with artificial rearing of camel calves. The research into non-invasive pregnancy diagnosis was carried out from 2010 to 2012. Urine from 14 camel females kept in four European zoological gardens was collected and tested using two chemical tests -- the Cuboni reaction and barium chloride test. The Cuboni reaction was significantly (p < 0.01) affected by the pregnancy status of female camels, and its accuracy increased significantly (p < 0.05) in the period leading up to parturition. The barium chloride test did not provide reliable results. Next, the saliva of five adult female camels was sampled for more than one year and concentrations of progesterone (P4) and oestradiol (E2) were measured. The concentrations of P4 (n = 312) and E2 (n = 310) were both significantly (p < 0.0001) affected by the pregnancy status of the animals. Maternal and suckling behaviour was observed from 2003 to 2009 in six zoological gardens, and the presented study includes partial data from this period. Allosuckling (i.e. when a female nurses a non-filial offspring) was described for the first time in camels and it represented 8.58% of all suckling bouts. The non-filial calves suckled more often in the lateral position and preferably joined the filial calf when suckling, so the results support the 'milk theft' hypothesis (stealing of milk) as a main cause of this behaviour. Finally, calf rearing in the Prague zoological garden was summarised and two camel calves were successfully artificially reared. This PhD thesis concluded that 1) the Cuboni reaction with urine and salivary P4 and E2 measurements are suitable methods for pregnancy diagnosis in half-tamed female camels; 2) allosuckling is relatively common in captive Bactrian camels; 3) the artificial rearing of camel calves with a calf milk replacer can be successful.
|
|
Evaluation of the effect of radiation on evapotranspiration estimates and drought indices
Mairich, Pavel ; Matula, Svatopluk (advisor)
Abstract Evaluation of the effect of radiation on evapotranspiration estimates and drought indices The severity of drought can be inferred from water balance, of which evapotranspiration is a component. The evapotranspiration estimates are often based on the FAO 56 methodology with the net radiation as the main input. Usually, however, the latter is not directly measured. This study investigates to which extent can the direct solar radiation and the long-wave net radiation measurements be replaced by calculation according to FAO 56 with constant or locally optimised radiation coefficients or, for the long-wave net radiation, the coefficients according to Penman (1948). The problem is demonstrated on data from the Solar and Ozone Observatory in Hradec Králové for 2011 and 2012. On average, the estimates of solar radiation are satisfactory even with the standard coefficients and can be improved by local optimisation of the coefficients. The estimates for particular days may considerably differ from reality. The long-wave net radiation estimate according to FAO 56 is, on average, by about 30 % lower than the measured long-wave net radiation or an estimate thereof based on locally optimised or Penman's coefficients, with the average differences between any two of the last three methods much smaller (less than 9 %). The inaccuracy of estimates for particular days is considerable, too. The average reference crop evapotranspiration according to FAO 56 with standard coefficients is therefore considerably higher (by about 15 %) than analogous evapotranspiration obtained from the measured radiation or according to FAO 56 with optimised or Penman's radiation coefficients. The cause is that grass in the observatory was not irrigated. It therefore occasionally suffered from water stress and got overheated. The use of FAO 56 with the radiation inputs measured or calculated using other than the standard radiation coefficients may underestimate the evapotranspiration and the need for irrigation.
|
|
Analysis of water requirements of the field crops in areas with frequent occurrence of drought.
Zábranský, Petr ; Soukup, Josef (advisor) ; Jan, Jan (referee)
Summary
Water resources are an important part of ecosystems. In the water balance of the landscape, the variability of climate, soil factors, as well as high diversity of vegetation play an important role. Evapotranspiration represents a major passive component in the water balance of the basin. Unlike forests, where the water balance is particularly predictable due to the long-term development of the stands, regular crop rotation in agricultural systems and agricultural practices may cause rapid changes in water balance during much shorter time. It may sometimes result in irreversible changes in ecosystems with impact on landscape biodiversity. An understanding of the limiting environmental factors and exact determination of the water demands of plants makepossible not only an improvement of yield parameters, but it is also condition for understanding of the agroecosystems sustainability in the cultural landscape.
Field measurements of actual evapotranspiration were carried out in the period 2007 to 2015 in Velvary (Budihostice) in the Central Bohemia region, 210 m above sea level in the rain shadow area around Slaný territory. In terms of the average annual water balance (CHMI, 2014), the locality is regarded as the area with the difference between the average annual precipitation and annual potential evapotranspiration totals in amount of -200 mm, so the potential evapotranspiration slightly exceeds precipitation. Field measurements were conducted on commonly managed areas with a minimum size of the experimental plots of 1 ha.
The measurements confirmed differences between the crop water demands during the growing season. Different values of the actual evapotranspiration as well as the Bowen ratio values were estimated for the observed crops depending on the growth stage of crops. The lowest values of actual evapotranspiration were detected for Zea mays and Sorghum bicolor. Higher water demands were shown by Triticum aestivum and Hordeum vulgare. On the contrary, the highest values of actual evapotranspiration were found in stands of Beta vulgaris and Medicago sativa. According to the values obtained for crop coefficients (Kc), the crops of Z. mays, S. bicolor and T. aestivum had about a quarter lower values of Kc compared with M. sativa and B. vulgaris crops in the main growing season. In this work, the daily values of Kc, which are the basic characteristics of moisture requirements for individual crop were determined and can be used modelling of hydrological processes in agro-phytocoenoses. The evaluation of moisture requirement of crops by the water flow in plants (determined by the sap flow method) showed the expected differences between S. bicolor and Z. mays plants. Water use efficiency (WUE) was significantly higher in plants of C4 type (Z. mays and S. bicolor) compared with the rest of observed plants of C3 type (T. aestivum and H. vulgare).
|
guest ::
