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The role of insect adipokinetic hormones in oxidative stress
VEČEŘA, Josef
In this work, the role of adipokinetic hormones (AKHs) in specific pathological conditions known as oxidative stress (OS) was evaluated in insects. A previous suggestion that the AKHs reverse the OS status elicited by the herbicide paraquat was confirmed using other OS elicitor insecticides malathion and endosulfan in the classical model species the firebug Pyrrhocoris apterus. Nevertheless, the main part of this work is focused on another insect model a herbivore Spodoptera littoralis (Lepidoptera), where the OS was elicited by tannic acid and where the impact of both OS and AKH treatment was evaluated in larval midgut tissue. Several markers of OS including activities of antioxidant enzymes, their gene expression, protein carbonyls and reduced glutathione level were measured. The results confirm the active role of AKH in activation of antioxidative defence reactions and suggest a pathway partially through which the antioxidant effects of AKHs could be realized.
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Marketing Plan of MAGICAM HD SOLUTIONS, Ltd
Pařízek, Aleš ; Hesková, Marie (advisor) ; Harantová, Monika (referee)
The main goal of the bachelor study is to create functional marketing plan for year 2017 that is consistent with the existing long-term marketing strategy. This thesis is created for use in business company MAGICAM HD SOLUTIONS Ltd. The core of the thesis is devided into theoretical and practical part. The first part laid the foundations basis of the terms used in the marketing planning, it is described the relationship marketing strategy, plan and tactics and role in the functioning of the marketing plan of the company. The second part contains the characteristics of the company, the analysis of external and internal factors affecting the company, SWOT analysis, setting goals and designing procedures by which the company will achieved set go-als. The practical part also contains specific steps in the action programs and timetable. Finally the thesis evaluates individual parts and contribution to the company.
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Design of methodology of administration and management of issues and its application in chosen organisation
Holub, Adam ; Stanovská, Iva (advisor) ; Novák, Tomáš (referee)
This thesis is focused on design methodology of administration and management of issues, using issue-tracking system. The objective is to create such methodology and deploy it to specific organization. During methodology creation, there were used best practices, acquired from source thesis and authors experiences. In theoretical part of thesis are described approaches, witch is this thesis based on. Then thesis describes what issue-tracking system is and lists mostly used issue-tracking systems. In practical part, the methodology itself is created. At first, target group is described. Then, types of issuse and roles used in issue-tracking system are described. Then is described, how to create lifecycle of issue and how to handle with its attributes. Last part is focused on data reporting from issue-tracking system. Last chapter of practical part describes how to implement methodology to issue-tracking system JIRA.
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Freedom of Expression and Censorship on the Internet and Social Networks
Reindl, Jan ; Čermák, Radim (advisor) ; Sova, Martin (referee)
This thesis is focused on freedom of expression and censorship on the internet and social networks. Its goal is to evaluate current situation of censorship of the internet, to describe the most often used methods of censorship and suppressing free speech, as well as methods of its circumvention. The thesis is focused on the progress of internet censorship in its shift from a tool of governmental domestic network control into a tool in the hands of multinational organizations. Some focus is also given to the importance of anonymity as a way to freedom of speech, and to the analysis of current situation and possible future outcomes. This thesis is divided into five chapters. The first one serves as an introduction into the problematics. Second chapter describes methods of internet censorship, the third one describes its circumvention. The fourth chapter looks into the current situation regarding the role of social media in terms of internet censorship and possible results of censorship as well as future evolvement. Fifth and last chapter is dedicated to research of anonymous parts of internet community that could otherwise face censorship.
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Health care professional as a patient
Uhlířová, Lucie ; Pečenková, Jaroslava (advisor) ; Ulrychová, Hana (referee) ; Švecová, Dagmar (referee)
The bachelor's thesis deals with the topic of a health care professional dealing with personal experience in a patient's role. The theoretical part presents facts associated with health and ilness psychology, as well as pieces of knowledge about human personality and a man's role in human society. It describes the special demands of general nurse's profession on a person. Special attention is paid to a situation when a nurse becomes a patient. The research part analyses the influnce of nurse's profession over her personal health and ilness perception. It describes difficulties that are experienced when a nurse is coping with her own hospital admission. The main goal of the thesis is to find out whether personal experience in a patient's role influences nurse's future life in any way.
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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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The effect of tick salivary proteins on innate immunity cells
PÁLENÍKOVÁ, Jana
Saliva of Ixodid ticks contains a whole array of pharmacologically active molecules with vasodilatory, antihemostatic, and immunomodulatory activities. This thesis focuses on two types of salivary proteins, serpins and cystatins, and their role in immunomodulation. These protease inhibitors are known to affect many biological functions. To better understand their role in tick saliva we examined their effect on dendritic cells and their ability to modulate the immune response after pathogen infection. As model pathogens, Borrelia spirochetes and tick-borne encephalitis virus were used.
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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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Vektorová segmentace objemových medicínských dat založená na Delaunay triangulaci
Španěl, Michal ; Martišek, Dalibor (referee) ; Sochor, Jiří (referee) ; Kršek, Přemysl (advisor)
Image segmentation plays an important role in medical image analysis. Many segmentation algorithms exist. Most of them produce data which are more or less not suitable for further surface extraction and anatomical modeling of human tissues. In this thesis, a novel segmentation technique based on the 3D Delaunay triangulation is proposed. A modified variational tetrahedral meshing approach is used to adapt a tetrahedral mesh to the underlying CT volumetric data, so that image edges are well approximated in the mesh. In order to classify tetrahedra into regions/tissues whose characteristics are similar, three different clustering schemes are presented. Finally, several methods for improving quality of the mesh and its adaptation to the image structure are also discussed.
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