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Separation Factors for Enrichment of Minor Components in Oils Extracted from Seeds with Supercritical CO2
Sovová, Helena
The present contribution shows that the same separation factors as for the oil fractionation in counter-current columns can be used to calculate the course of extraction of triacylglycerols and minor oil components in the initial period of fast extraction from crushed seed. Thus, the optimal conditions for minor component pre-concentration in extract or for removal of free fatty acids from the oil can be predicted. The values of separation factors for different minor components and for oils consisting mainly of C18 fatty acids are presented in dependence on the temperature, pressure, and CO2 density. The separation factors are based on both literature data and experimental data measured in our laboratory. The application of separation factors to calculate extraction yields of oil and its minor components in dependence on the amount of passed CO2 is presented, too.
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Řízení IS/ICT se zaměřením na sourcing služeb informačního systému
Šebesta, Michal ; Voříšek, Jiří (advisor) ; Havlíček, Zdeněk (referee) ; Příklenk, Oldřich (referee) ; Král, Jaroslav (referee)
Research on outsourcing has been around for several decades, while recent evolution in the information systems discipline towards ICT service commoditization significantly changes the context of decision-making. Services that are available on-demand via the Internet allow organizations implementing functions they demand in a fraction of time. This trend represents a chance for organizations seeking to use advanced ICT services without a need of major investments. Problem is the current lack of guidelines and tools for managing ICT services and their outsourcing. Given the trends on the ICT service market, it is expected that much of the IT management in the future will encompass the ICT services and utilize service-level structures. Methods currently available are either too broad or encompass only small part of the whole problem. Ad-hoc or unsound decisions in this area might cause major complications in terms of quality, usability, integration, and consequently influence total cost of organizational IT. Organizations need to either revise existing models or propose and implement completely new models to manage their IS/ICT. This thesis deals with the management of IS/ICT with focus on the ICT services outsourcing. It discusses available sourcing models in the literature and links them to the various interconnected areas. Based on these areas, it presents an integrated view on IT outsourcing strategies. Most importantly the thesis proposes an original concept for decision-making about outsourcing of ICT services named the SOURCER framework. This approach utilizes the presented outsourcing strategies, and introduces a complex methodology and decision-making criteria that will assist organizations with selection of ICT services in order to maintain and manage a most suitable ICT service portfolio. The decision-making is based on four essential viewpoints: function, costs, time, and quality. These viewpoints are discussed, individually analyzed, and serve as a basis for further research. The whole framework is developed and validated according to Design Science Research Methodology (DSRM). Individual components are evaluated using a survey among a group of selected IT managers. Proof of concept is then established by a case study on framework use in a real organization. This case study covers strategy specification, business--IT alignment, specifying service architecture and its interconnections, outsourcing, and management of the ICT service portfolio.
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Usage of unstructured data in Business Intelligence
Rakhmanova, Malika ; Šperková, Lucie (advisor) ; Karkošková, Soňa (referee)
The aim of the thesis is to identify the main trends that are occurring in the market of Business Intelligence and related to unstructured data, to describe the possibilities for integrating unstructured data, to clarify what the impact on the company have the results that can be obtained using these solutions and how generally incorporate an analysis of unstructured data into BI. Another aim is to show the current situation of processing unstructured data on the example of BI system. The thesis is divided into several parts. First part is describing of the Business Intelligence area and the basic components of Business Intelligence, as well as identifying market trends. Then, there is the next part: separating the data into structured and unstructured. Here is the part about how you can access and analyse unstructured data and what is their place in BI systems. This is the end of a block of unstructured data and the beginning of a description of the enhanced version of BI. Finally, the current market situation and BI tools, which include unstructured data, are introduced. This section provides an overview of how BI tools approach to analyse unstructured data. Existed literature, professional and freely available Internet resources are used for writing the work. The purpose is to serve as a source of information for quickly orienting in the current situation, to serve as a guide to the world of BI solutions and to show potential users what are the options and functionality of these BI solutions.
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Expected development of Business Intelligence applications in the coming years
Aschmann, Jakub ; Pour, Jan (advisor) ; Fortinová, Jana (referee)
This thesis focuses on the development of Business Intelligence applications. The main area is the potential development in the coming years, history of business intelligence and individual components. To acquire the necessary knowledge, I studied educational materials and electronic publications. In Chapter 3 I focus on the definition of BI, in the following chapter I analyse individual components and the last chapter is dedicated to the development in the future. This thesis will provide comprehensive knowledge and trends in business intelligence to readers who are interested in this area and are looking for complex information.
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DOM optimization
Mironov, Nikita ; Veber, Jaromír (advisor) ; Luc, Ladislav (referee)
The thesis's main theme is comparison of different approaches of work with DOM API. The aim of the theoretical part of the thesis is explanation of the most popular web browsers' architectures and their inner components. Also, thesis describes the DOM standard and most common optimizations.
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Design of marketing strategy in the company Imperium Finance
Petrovič, Marko ; Čermák, Radim (advisor) ; Sova, Martin (referee)
Internet marketing is currently one of the most important components of marketing. However, Internet marketing as a whole is much more important than mere webdesign, mere search engine optimization (SEO) or a simple PPC advertising. Most companies have recently made it throught with a website and that was all their presentation on the Internet. However, the internet is one of the fastest growing technology today and the number of its users is growing every year. Constantly there are new technologies that allow us to have a better user experience and also bring us new ways of presentation and promotion. Most of the companies make a mistake early on and underestimate the preparation, don't know their goals, customers or competitors. Internet marketing is the process and the contents of this process is the recognition, analysis, design, implementation, and ultimately verify that you achieve the goals of the organization. In the theoretical part it is defined internet marketing and its advantages over offline marketing. Briefly sum up the history and major milestones of Internet marketing that shaped its present form. Further, it's described current trends in Internet marketing and detail expands upon the selected Internet marketing tools and tools for evaluating the success of campaigns. The practical part deals with the proposal of marketing strategy in the company Imperium Finance. The main objective is to analyze the requirements of the company Imperium Finance, from analysis to draw specific conclusions and devise a marketing strategy that will support the vision and strategy of the company in the following period. All steps were carried out within the company Imperium Finance on real data from active clients and tested in action. The result is a proposal of a marketing strategy that will drive Imperium Finance, and a marketing plan for the company. The biggest benefit of this work is so real use theoretical knowledge in practice.
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Geodiversity values as a basis for geosite and geomorphosite assessment: a case study from Žďárské vrchy Highland
Bajer, A. ; Kirchner, Karel ; Kubalíková, L.
Paper presents geodiversity values, geodiversity is understood as a set of geological, geomorphological and pedological components, including systems consisting of this components and geological, geomorphological and pedological processes. Geodiversity values are analyzed in relation to ecosystem values (e.g. cultural, functional value). Geodiversity values were used as basis for a methodical approach to geosite and geomorphosite assessment. Methodological approach is presented at selected sites in the top part of the Žďárské vrchy Highland. The acquired results can serve as a basis for geoconservation of PLA Žďárské vrchy, development of geotourism as well as an offer for extension geoeducational activities.\n
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Effect of low doses of herbicide paraquat on antioxidant defense in Drosophila
SÁBOVÁ, Michala
Disruption of cell equilibrium between production of free radicals and antioxidant defence is named oxidative stress. The main component of antioxidant mechanism is activity of antioxidant enzymes, which include superoxide dismutase (SOD) and catalase. In this study we evaluated the effect of herbicide paraquat on response of SOD and catalase, their transcription level and locomotion activity in Drosophila melanogaster. We exposed the flies to a wide range of paraquat concentration. Our results revealed that transcript and enzymatic levels of both SOD and catalase have a similar biphasic dose response with the peaks at 2,5 ?M paraquat concentration, resembling hormetic effect. Then, males were more sensitive than females. However, females had an increased locomotion activity. We found that paraquat susceptibility is increased in males and mating flies. Therefore, this study supports hypothesis of stress sensitivity elevation as a physiological cost of reproduction.
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PM2.5 Chemical Composition at the Rural Background Site Košetice.
Schwarz, Jaroslav ; Cusack, Michael ; Karban, Jindřich ; Chalupníčková, E. ; Havránek, Vladimír ; Smolík, Jiří ; Ždímal, Vladimír
Measurements of atmospheric aerosols at rural background areas are important because they are not influenced by the urban emissions, and are therefore better suited to investigate the influence of long-range transport and trends in concentrations of individual pollutants. Summary of the measurements of total concentration and the concentration of PM10 and PM2.5 components was carried out in the works Putaud et al. (2004, 2010). Although the later work included the measurement of more than 60 stations across Europe, the post-communist countries contained data on PM2.5 from only four locations (Prague, Debrecen, Hungary, and two rural locations). Although Košetice place where this study was made, is also discussed there, it was for only PM10 and total carbon (TC). Therefore, the aim of this study is to fill this information gap, and provide additional information regarding the PM2.5 fraction and composition at the Central European background station Košetice.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016110313110 - PDF
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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