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Přirozeně se vyskytující toxiny v potravinách
Pavlíková, Lenka
The bacherol thesis Naturally occurring toxins in food concerns the different toxins. Plant toxins is described and classified by chemical nature. It also deals with t fungal toxins and toxins of microorganisms, mycotoxins and bacterial toxins. The work also describes the toxins of marine animals. Where they occur. Their effect and characteristic signs of repair. Finally, I is written measures that are prevention of toxins in food.
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Jsou parmy obecné v době výtěru "jedovaté" pro ostatní vodní organismy?
ŠVEJDA, Pavel
This bachelor thesis deals with fish toxicity with the emphasis on the barbel (Barbus barbus Linnaeus, 1758). Its tissues and especially roe cause poisoning to human known as a "barbel cholera". Possible lethal effects of barbel the killer shrimp Dikerogammarus villosus (Sowinsky, 1894), the marbled crayfish Procambarus virginalis Lyko, 2017 and the round goby Neogobius melanostomus (Pallas, 1814) were examined in this thesis. Different types of tissues and early life stages of barbel were used due to the indistinct distribution of possible toxin in fish body. In addition, using a behavior test, where marbled crayfish were used as an experimental organism, we monitored possibilities of toxin detection by consumer. Our results suggest that there is no evidence of barbel lethal toxicity on chosen consumers. We also did not find evidence that crayfish are able to recognize flesh of ready and unready to reproduce barbel. To sum up, we did not find evidence that toxin which is present in barbel body affect given consumers. It can be given either their co-evolution (arms race) or their ancestors had never been susceptible on this toxin. Thus, there can only be a spontaneous effect of mentioned toxin on mammals or humans.
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The role of fatty acylation in activity of proteins
Grobarčíková, Michaela ; Mašín, Jiří (advisor) ; Petráčková, Denisa (referee)
Post-translational modifications are covalent and generally enzyme-mediated modifications of proteins. These modifications can serve to create active forms of proteins and they can also expand the cellular repertoire of proteins derived from standard amino acids. Known post-translational modifications include for example phosphorylation, glycosylation, ubiquitination, proteolysis and also acylation discussed in more detail in this thesis. Acylation of proteins, the covalent attachment of an acyl group, is a very frequent protein modification. This reaction is typically mediated by specific acyl transferases and involves transport of an acyl group from a donor to an amino acid residue. A diverse spectrum of cellular proteins is post-translationally acylated and therefore become biologically active. This phenomenon occurs in bacterial toxins, which are important factors of the virulence of pathogenic bacteria. Protein lipidation regulates numerous biological pathways such as membrane targeting, protein secretion, cell signaling, and apoptosis. Posttranslational acylation is also required for Ras activity and many other cancer-causing proteins. Therefore, inhibitors of acyltransferases of these proteins are being tested as targets for antitumor drugs. In this work, findings about individual types of...
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Tetanus: known and unknown disease
HOSNEDLOVÁ, Barbora
Tetanus is a life threatening disease caused by a toxin produced by the ubiquitous bacterium Clostridium tetani. The aim of this bachelor thesis was on the basis of ... to get information about causes of tetanus among animals in the Czech Republic and to evaluate it. There were 40 filled out questionnaires collected in total. The majority of respondents (72.5%) knew that tetanus is a dangerous disease not only for humans, but also animals. The most often affected animals were dogs (11; 27.5%), goats (10; 25%) and horses (9; 22.5%). Breeds that were affected the most were Czechoslovakien shepherd and Border collie among dogs, then White/Brown shorthaired and Anglonubian goats and Czech warmblood horses. The first described clinically symptoms were cramping in facial muscles, straightening of the ears or cramps. There have also been less typical symptoms like vomiting, swellings of the head, or within herd animals, avoiding the herd. All respondents have stated they would appreciate more information about the disease.
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Laboratory diagnosis of Clostridium difficile
JANDOVÁ, Romana
Clostridium difficile toxin with production is the most common cause of nosocomial enteric infections. It causes inflammatory bowel disease called Clostridium difficile infection (CDI) of varying severity - from trivial diarrhea to life-threatening conditions such as paralytic ileus and toxic megacolon. C.difficile still escapes the attention of the general public and is in the background of other bacteria, such as MRSA. Clostridium difficile is a strictly anaerobic bacterium. It is a gram-positive rod forming an oval subterminal spores. C. difficile can produce two types of toxins - and toxin A (enterotoxin) and toxin B (a cytotoxin). Due to the toxin causes damage of the intestinal epithelium and deeper layers of the bowel wall. Into inflammatory ulceration that cover of the pseudomembrane. Some strains produce more binary toxin, whose exact function is not underwood yet. It is assumed that potentiates the effect of toxins A and B to increase their concentration. To demonstrate the CDI must remove the stool sample into a sterile tube. In the bacteriology workplace in Czech Budweis Hospital is being made direct conclusiveness of antigen and toxin by immuncgromatography method. It is a membrane-enzyme immunoanalysis for the detection of antigen - glutamate dehydrogenase (GDH) and toxin A / B. Negative result excludes GDH with high reliability clostridial infection. For confirmed CDI is considered proof toxigenic strain of C. difficile. This is evident from the result of either immunochromatography which is demonstrated both the antigen and the toxin, or identity of the gene for toxin B by PCR. If you find out in direct detection only positive antigen, the sample is sent for PCR testing to the Laboratory of Molecular Biology and Genetics. Regardless of the outcome of the immunochromatography, the microscopic specimen stained by Gram are being produce from reces. In the case of proven C. difficile toxin is carried anaerobic culture test that takes two days. For identification of accrued bacteria is used latex agglutination. This is a method in which IgG antibodies bound to latex particles specifically bind to the antigens of the cell wall. To identify the bacteria can be also used the VITEK - MS machine that uses a laser ionization method in the presence of the matrix, followed by mass spectrometry. In case of a positive culture findings Clostridium difficile is being from grown culture set sensitivity to antibiotics by E-test and disk diffusion methods For the year 2011 the Department of Bacteriology examined 291 samples of feces. 13,4 % of these samples had positive antigen and toxin in an imunochromatographyc examination; 15,1 % had a positive antigen and negative toxin and 71,5 % were negative. 74 samples was positive in C. difficile toxin. These samples were subsequently conducted to culture examination - 62,2 % were positive. Most positive samples were sent from the infectious department. It has been examined 53,6 % of women and 46.4 % of mens. Percentage of toxigenic strains of both sexes were virtually identical - 25 %. All strains were susceptible to vancomycin and only one strain was resistant to metronidazole.
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Expression of Kunitz-type recombinant protein, potential toxin from the salivary glands of the tick \kur{Ixodes ricinus}
CERMANOVÁ, Tereza
Ticks feed on their vertebrate hosts for number of days, providing enough time for development of an effective immune response. To overcome this, tick saliva contains a complex mixture of active substances which mediate host defense mechanisms. Extremely important role at the tick-host interface is played by the protease inhibitors. In this work, we have focused on the protein named Ixocludin 2, a member of the Kunitz type/Bovine trypsin inhibitors of serine proteases, related also to the potassium channel blockers toxins. Three different expression systems (Escherichia coli, Chinese hamster ovary and Pichia pastoris) were tested to prepare an active recombinant Ixocludin 2, out of which, only bacterial system was in part successful.
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Biological Weapons and Readiness of Integrated Rescue System for Biological Attack
KVÍDEROVÁ, Lenka
As the topic for my Bachelor Thesis I have chosen the Biological Weapons and Readiness of Integrated Rescue System for Biological Attack. The bioterrorism has recently become one of the most critical worldwide problems. The terrorist organizations that would misuse the biological weapons as a tool of pressure and blackmail to enforce their own interests would choose mainly civilian population as the target of their attack. The Integrated Rescue System plays an important role in protecting the population against this threat. The purpose of this Thesis is to point out the issue of biological weapons, to outline possibilities of production of these hazardous materials, to inform about how these biological means can be spread among the population and mainly to draw attention to the most significant representatives of the biological weapons. I will also describe the Integrated Rescue System and its individual components taking part in solving extraordinary situations incorporating occurrence of B-agens. In order to ensure readiness of the basic components of the Integrated Rescue System for biological terrorism, there has been a questionnaire compiled and handed over to the respective members of the Fire Brigade, Police of the Czech Republic and medical emergency services to fill it in. The Thesis is verifying the hypothesis whether the Integrated Rescue System components are sufficiently ready for a biological attack. Based on confrontation of the information obtained from literature, legislation, electronic sources and outcome of the questionnaire survey, there was an answer to the defined hypothesis formulated in the conclusion, stating that the Integrated Rescue System components are not currently prepared sufficiently for a biological attack.
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