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Probiotics and prebiotics in the diet of the population. Effect on disease frequency.
Cherepanova, Ekaterina ; Starnovská, Tamara (advisor) ; Horová, Eva (referee)
Over the past decade, scientific research has focused on the intricate relationship between the human microbiome and the immune system. A particular emphasis in the last three years has been on investigating the impact of the microbiome on the susceptibility and severity of COVID- 19 infections. There is substantial evidence suggesting that the consumption of fermented foods, ranging from fermented vegetables and kefir to yogurt and kimchi, has a beneficial effect on the composition of the intestinal microbiome and, consequently, on the function of the immune system. This study, part of a bachelor's thesis, explores the complex association between regular consumption of fermented foods and the frequency of infectious diseases. Through the analysis of data obtained from a questionnaire, it was found that individuals who regularly incorporated fermented products into their diet exhibited a lower frequency of infectious diseases over the past two years. These results indicate that the consumption of probiotics and prebiotics may positively influence the immune system, reducing the risk of disease. Regarding the examination of the relationship between the consumption of probiotics from food sources and the frequency of COVID-19 infections, a contrasting observation emerged. Those who were fully...
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Importance of probiotics in child's nutrition
Augustová, Kamila ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
With great advances in science, medicine and knowledge of a man at the high level of treatment. However, even today the humanity seeking to return to its roots - the nature and use it to maintain its good health. The term probiotics is meant by living organisms that are capable of resettling in the human intestine and have a positive impact on maintaining healthy intestinal microorganisms, which also reflected the positive effects on human health in general. Company Valosun a.s. provided for testing probiotický food supplement for children, which was tested mothers with children preschool age and pregnant. The aim of our sociological survey was to determine what is the awareness of the issue of probiotics in this group, the perception of the queried the availability of probiotics and what are the most common reasons for their use.
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Probiotics and their importance in human nutrition
Formanová, Kristýna ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
A great deal of immune system is located in the colon thanks to a large number of microbs that influence not only digestive tract of a human. The contact of these microbs with the digestive tract of a human is very important for other development of immune defensive mechanisms and the natural immune response, which supports health of a host. That is why in recent years a great deal of attention and efforts have been paid to possibility of human health status improving by a modulation of the intestinal microflora. As probiotics are indicated living microorganisms, that can have positive effects on human health and some species, for example Saccharomyces boulardii, have positive effects in prevention and treatment of many diseases associated with diarrhea. The company Valosun a.s. has provided for testing the probiotic food supplement which is suitable in acute diarrheal disorders. This product has been tried by patients of gastroenterology ambulance. The aim of sociological survey was to map knowledge of respondents in the field of probiotics and probiotic food supplements.
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Vliv změny stravovacího režimu na vybrané ukazatele mikrobiomu lidského trávicího traktu
Patloka, Ondřej
The literature review of this thesis discusses the intestinal microbiome of the human gastrointestinal tract. The main attention is paid to the chapters on the development of the intestinal microflora within the life stages of a person, the importance of the microbiota in terms of metabolism and influencing the health of the host, and the influence of the way of eating in terms of the intake of basic nutrients and specific food components of the diet, which can change the bacterial diversity and composition of the intestinal microflora. In the practical part, a microbiological analysis of the fecal microflora of six individuals (n=6) who underwent a weight reduction course was performed. During the experiment, 3 microbiological analyzes of the stool of the probands were performed within the monitored period, i.e. (at the beginning of the weight reduction course, at its end and in the period after the end of the weight reduction course). Microbiological analysis was performed by the plate method, where the number of KTJ/g stool of the probands was evaluated after cultivation. For each of the probands, one sample was analyzed in each phase of the experiment. For statistical evaluation, the averages of log KTJ/g from the stool of all probands within each phase of the experiment were used. At the same time, there was a detailed evaluation of the intake of basic nutrients and other food components of the diet from the dietary records of the probands within the individual periods of the experiment. At the end, a correlation analysis was performed between the observed parameters. During the experiment, there was a decrease in the intake of total carbohydrates, monoenoic and trans fatty acids (P<0.05). In the group of probands, a tendency to decrease total energy intake and increased fiber intake was also noted. As part of the reduction course, there was a decrease in the abundance of Bacteroides spp. and lactic acid bacteria (P<0.05). In contrast, the numbers of Lb were increased at the end of the reduction program. acidophilus and Lb. rhamnosus in the stool of probands (P<0.05). In addition, in the period after the end of the reduction course, there was an increase in the abundance of fecal Bifidobacterium spp. (P<0.05) and a reduction in the number of Enterococcus spp. in the proband group (P<0.05). Based on the detected changes in the abundance of the bacterial composition of the cultured taxa, a possible reduction in the body weight of the probands within the monitored experiment can be assumed.
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Effect of intestinal microflora on the activity of higher brain centers
Arnold, Tomáš ; Hock, Miroslav (advisor) ; Melkes, Barbora (referee)
Abstract Intestinal microflora consists of large bacterial community that resides in the intestines, especially the colon, and lives in symbiosis with the host. It consists mainly representatives of the four bacterial strains (Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria). It has already been shown that the proportional representation of the bacterial strains in time does not change much, but the research of the last decade have shown irrefutable effect between the change of diet and composition of the intestinal microflora. In addition, there is evidence of changes in the representation of bacterial strains in the course of development of certain intestinal pathologies such as inflammatory bowel disease. More and more are starting to emerge papers describing the influence of altered intestinal microflora respectively its representatives, on the activity and function of the CNS. This interaction of the digestive system and CNS seems to be bidirectional and mediated by several different pathways. Finally, it shows that dysbiosis of the intestinal microflora promotes anxiety and depression that can lead to the urge to prematurely terminate life. The aim of this work is to provide a comprehensive view of this relatively young, but also very interesting...
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Influence of commercial probiotic preparations on human intestinal microbiome composition
Balatka, Štěpán ; Hodek, Petr (advisor) ; Osičková, Adriana (referee)
The intestinal microflora is an extensive ecosystem of microorganisms that consists of symbiotic and pathogenic species. The microflora is responsible for many important functions in the human body. An unquestionable function is that it affects the health state of the host. The higher the biodiversity, the greater the benefit for the host. However, it is necessary to point out that this should not include a high diversity of pathogenic bacterial species. There are many "beneficial" species, especially from the Bifidobacterium and Lactobacillus families. In recent decades, the popularity of supplementing these "beneficial" species with various supplementary diets (e.g. probiotics) has been growing a lot. The presented diploma thesis deals with pilot studies of liquid commercial probiotic preparations from American companies Ascended Health (not available on the Czech market) and their effects on the human microbiome. The study involved 9 volunteers who provided 70 fecal samples before, during, and after use of the studied products. Two methods were used to determine the biodiversity of intestinal bacterial species. Both are based on the identification by bacterial DNA that encodes gene 16S rRNA. The first method uses PCR-DGGE technique and then identification by Sanger sequencing. The second method...
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Probiotics, their influence on health and probiotic food
Hondlíková, Tereza ; Dostálová, Jana (advisor) ; Plocková, Milada (referee)
Probiotics are living microorganisms which show positive effects on human health if consumed in a sufficient quantity. Probiotics have been under a lot of research recently, however their effectiveness in terms of treatment or prevention of diseases has not been supported by a sufficient number of studies and research yet. The thesis summarizes the existing knowledge about probiotics, characterizes individual types of probiotic organisms, describes possible sources of probiotics and their impact on human health. Theoretical part of the thesis deals with the definition of the concept of probiotics, also describes prebiotics and synbiotics which are closely related to this topic. characteristics of individual groups of probiotics as well as their impact on human health and possible use in the treatment of diseases as a form of biological treatment are also included. Furthermore the sources of probiotics both in the form of foods containing probiotics and in the form of probiotic dietary supplements are described. At the end of the theoretical part, recommendations for receiving and selecting a suitable source of probiotics are summarized. The aim of the practical part was to find out if the general public is aware of probiotics. Questions about preferences in the selection and consumption of...
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Role vybraných druhů Lactobacillů na fyziologii a imunitu tenkého střeva člověka a prasete
Moravcová, Eliška
These bachelor thesses deals with the effect of four chosen species from genus Lactobacillus on immunity system and homeostasis of small intestine in human and pigs. For this thesses were selected four species: Lactobacillus casei, L. acidophilus, L. rhamnosus and L. helveticus. The genus Lactobacillus and its probiotics impacts are very discussed topics within society and scientists teams. Although awareness of effects of Lactobacillus is on high level some facts are not completely understood or expanded to public. This work deals with, and also summarizes the current state of scientific research about the effects of Lactobacilli on human health and the orga-nism of pigs.
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Vliv probiotik a prebiotik na imunitní systém člověka
Pavlíčková, Tereza
This bachelor thesis The effect of probiotics and prebiotics on the human immune system is concerned with their beneficial effects on human health and used in the treatment of many diseases. The first part describes the intestinal microflora and the properties and effect of probiotics. Also individual probiotic strains and characteristics and properties of prebiotics and their species are harvested there. The last part describes the occurrence of probiotics and prebiotics in the food industry and in various dietary supplements.
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Interaction of gut microbiota with selected flavonoids
Balatka, Štěpán ; Hodek, Petr (advisor) ; Koblihová, Jitka (referee)
The microflora located on the surface of and inside the human body is a very large system. The intestinal microflora of the gastrointestinal tract is a very important and still not fully explored area. The intestinal microflora consists of many types of bacteria, viruses, fungi or some single-cell eukaryotic heterotrophic organisms. The intestinal microflora is involved, for example, in the synthesis of vitamins, digestion of polysacharides or in the storing of fats. It is significantly involved in the metabolism of foreign substances and digestion of food. The activity of foreign substances can influence the composition of the intestinal microflora. It must not be forgotten that the composition of the intestinal microflora differs in separate individuals and is changeable during life. The metabolism of foreign substances in relation to the human organism is a subject of global studies. This work is devoted to the metabolism of myricetin and its influence on the digestive tract of a healthy individual and a person with celiac disease. It is also studied whether myricetin affects the biodiversity of intestinal bacteria. The collected stool samples from donor A (a person with celiac disease) and B (a healthy control) were incubated at 0, 3 and 6 hours in McDougall buffer with myricetin. They were...
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