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Karyotype evolution of selected families of entelegyne spiders
Kotz, Matěj ; Král, Jiří (advisor) ; Nguyen, Petr (referee)
The Araneoidea superfamily is a diverse clade of spiders with a great species diversity. The whole superfamily displays considerable conservativeness of observed karyotypes. Most likely ancestral karyotype in males is 24 acrocentric chromosomes with X1X2 sex determination system. The goal of this study is to explore the karyotype diversity of two araneoid families - Araneidae and Mimetidae. The majority of studied species exhibit the ancestral karyotype. In some species of the aformentioned families was observed sudden increase in chromosome numbers, up to 2n♂ = 52 in Araneidae and up to 2n♂ = 57 in Mimetidae. The latter number is the highest chromosome count observed in Entelegynae so far. Increase in 2n goes hand in hand with increase in sex chromosome numbers, leading up to X1X2X3X40 system in Araneidae and up to X1X2X3X4X5X6X70 in Mimetidae. I suggest polyploidy as a possible mechanism of the increase. To test this hypothesis, I measured the size of the genome using flow cytometry and used fluorescence in situ hybridization for the detection of 18S rRNA and 5S rRNA genes. For one species, probe for U2 snRNA gene was also optimized as part of this thesis. In many species studied, these techniques were used for the first time ever. In the case of the family Mimetidae, the largest genomes in...
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Centric fusions in karyotype evolution of spiders
Mlnáříková, Barbora ; Forman, Martin (advisor) ; Nguyen, Petr (referee)
Entelegynae are the most specious group of spiders. Their karyotypes are characterized by the predominance of X1X20 sex chromosome system and exclusively acrocentric morphology of chromosomes. Mono-armed chromosomes can be the substrate for centric fusion, the rearrangement, in which two acrocentric chromosomes fuse at their centromeric regions and form single bi-armed chromosome. In Entelegynae, centric fusion events followed the "all or nothing" rule, where all acrocentric chromosomes fuse during one event or in a very rapid sequence and karyotype is fully saturated by bi-armed chromosomes. Although the cytogenetics of Entelegynae is relatively well studied, so far, "all or nothing" phenomenon has been observed only rarely, namely in 12 species from 5 families. In this thesis, new data about the occurrence of centric fusion in 12 species from 5 unrelated families are reported. In all cases the "all or nothing" rule was confirmed. Strong female meiotic drive is proposed as the main force responsible for changes in chromosomal morphology of Entelegynae. The persisting of bi-armed chromosomes is likely to be rare in Entelegynae. However, in the family Dictynidae, bi-armed chromosomes are fixed. For a better understanding of karyotype dynamics in this unique family, molecular cytogenetic approaches...
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Evolution of sex chromosomes and karyotypes in the lizard clade Laterata
Buchbauerová, Lucie ; Kratochvíl, Lukáš (advisor) ; Šťáhlavský, František (referee)
The aim of this study was to summarize all what is known about the karyotype and about sex chromosomes in the lizard clade Laterata and to analyze this two gained datasets by means of a phylogeny. Karyotype represents a whole number of chromosomes in a cell and it is an important source of informations on how the genetic information in nucleus is organized and how it works along with the evolution. The Laterata clade consists of four big groups of squamate reptiles: Teiidae, Gymnophthalmidae, Amphisbaenia and Lacertidae. All the groups have a genotypic sex determination system (GSD) and some species within these clades posses sex chromosomes. If they are present, in Teiidae and Gymnophthalmidae it is the XY sex chromosome system which determines the sex of a specimen. On the other hand, in Amphisbaenia and Lacertidae it is the ZW system of sex determination. Sometimes the X1X1X2X2:X1X2Y or Z1Z1Z2Z2:Z1Z2W sex chromosome system occurs in Teiidae / Gymnophthalmidae or in Lacertidae, respectively. The phylogenetical analysis was performed in Mesquite programme and the output from the analysis is a phylogenetical tree with two characters (number of chromosomes and the sex determination system) and the maximum parsimony counted for each of them. Then the interpretation was on the order and it is the main...
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Karyotype evolution of selected families of entelegyne spiders
Kotz, Matěj ; Král, Jiří (advisor) ; Nguyen, Petr (referee)
The Araneoidea superfamily is a diverse clade of spiders with a great species diversity. The whole superfamily displays considerable conservativeness of observed karyotypes. Most likely ancestral karyotype in males is 24 acrocentric chromosomes with X1X2 sex determination system. The goal of this study is to explore the karyotype diversity of two araneoid families - Araneidae and Mimetidae. The majority of studied species exhibit the ancestral karyotype. In some species of the aformentioned families was observed sudden increase in chromosome numbers, up to 2n♂ = 52 in Araneidae and up to 2n♂ = 57 in Mimetidae. The latter number is the highest chromosome count observed in Entelegynae so far. Increase in 2n goes hand in hand with increase in sex chromosome numbers, leading up to X1X2X3X40 system in Araneidae and up to X1X2X3X4X5X6X70 in Mimetidae. I suggest polyploidy as a possible mechanism of the increase. To test this hypothesis, I measured the size of the genome using flow cytometry and used fluorescence in situ hybridization for the detection of 18S rRNA and 5S rRNA genes. For one species, probe for U2 snRNA gene was also optimized as part of this thesis. In many species studied, these techniques were used for the first time ever. In the case of the family Mimetidae, the largest genomes in...
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Robertsonian translocations and their involvement into genome evolution
Mlnáříková, Barbora ; Forman, Martin (advisor) ; Sember, Alexandr (referee)
Chromosome aberrations play an important role in human pathologenesis as well as in evolution of many organisms. Robertsonian translocation or centric fusion are the most frequent chromosomal rearrangements in mammals. The most investigated model for their research is house mouse Mus musculus. The standard karyotype of a mouse consists from 40 acrocentric chromosomes, notably there are more than one hundred wild populations that are characterized by various combination of metacentrics, formed by centric fusion of different acrocentrics. Several models have been proposed for the formation of metacentrics, taking account of the sequential structure of their centromeres and short arms in mouse. Segregation distortion in favor of metacentrics is present in female and/or male meiosis, such as meiotic drive can positively affect the degree of fixation of metacentric chromosomes in certain population. Centric fusions have been also presented in evolution of other organisms. Cytogenetic data in spiders indicate the possibility of similar phenomena in the most diversified group of spiders, Entelegynae. Spiders are notable for their unusual sex chromosome systems. Centric fusions also play an important role in the evolution of sex chromosomes and the formation of neo-sex chromosome systems. So far, there is...
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Analysis of sex chromosomes and gene clusters in selected mygalomorph and araneomorph spiders
Pappová, Michaela ; Král, Jiří (advisor) ; Johnson Pokorná, Martina (referee)
1 Abstract: The diploma thesis focuses on study of sex chromosomes evolution and repetitive organized genes of chosen mygalomorph and araneomorph spiders. Spiders are characterized by complexicity of sex chromosome systems, their karyotypes contain multiple sex chromosomes X. Besides multiple X chromosomes they also contain a pair or two pairs of nondiferentiated sex chromosomes X and Y. The used methods include methods of classical cytogenetics (preparation of chromosome slides, C-banding) and methods of molecular cytogenetics (fluorescent in situ hybridization and comparative genome hybridization). Complex sex systems were discovered in the studied Theraphosidae spiders. In Theraphosidae spiders Atropothele socotrana and Poecilotheria vittata neo-sex chromosomes were found. Analysis of molecular differentiation of sex chromosomes suggests low differentiation of Y chromosome in neo-sex chromosomes and pair of nondifferentiated sex chromosomes XY. In haplogyne spider Kukulcania aff. hibernalis (X1X2Y), the Y chromosome was significantly differentiated, male specific signal covered the whole chromosome. Detection of 18S rDNA showed that karyotypes of majority of analysed Theraphosidae spiders and haplogyne spiders contain low number (1 or 2) of nucleolar organizing regions localized terminally, which...
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Evolution of sex chromosomes and karyotypes in the lizard clade Laterata
Buchbauerová, Lucie ; Kratochvíl, Lukáš (advisor) ; Šťáhlavský, František (referee)
The aim of this study was to summarize all what is known about the karyotype and about sex chromosomes in the lizard clade Laterata and to analyze this two gained datasets by means of a phylogeny. Karyotype represents a whole number of chromosomes in a cell and it is an important source of informations on how the genetic information in nucleus is organized and how it works along with the evolution. The Laterata clade consists of four big groups of squamate reptiles: Teiidae, Gymnophthalmidae, Amphisbaenia and Lacertidae. All the groups have a genotypic sex determination system (GSD) and some species within these clades pones sex chromosomes. If they are present, in Teiidae and Gymnophthalmidae it is the XY sex chromosome system which determines the sex of a specimen. On the other hand, in Amphisbaenia and Lacertidae it is the ZW system of sex determination. Sometimes the X1X1X2X2:X1X2Y or Z1Z1Z2Z2:Z1Z2W sex chromosome system occurs in Teiidae / Gymnophthalmidae or in Lacertidae, respectively. The phylogenetical analysis was performed in Mesquite programme and the output from the analysis is a phylogenetical tree with two characters (number of chromosomes and the sex determination system) and the maximum parsimony counted for each of them. Then the interpretation was in the order and it is the main...
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Evolution of sex chromosomes and karyotypes in the lizard clade Laterata
Buchbauerová, Lucie ; Kratochvíl, Lukáš (advisor) ; Šťáhlavský, František (referee)
The aim of this study was to summarize all what is known about the karyotype and about sex chromosomes in the lizard clade Laterata and to analyze this two gained datasets by means of a phylogeny. Karyotype represents a whole number of chromosomes in a cell and it is an important source of informations on how the genetic information in nucleus is organized and how it works along with the evolution. The Laterata clade consists of four big groups of squamate reptiles: Teiidae, Gymnophthalmidae, Amphisbaenia and Lacertidae. All the groups have a genotypic sex determination system (GSD) and some species within these clades posses sex chromosomes. If they are present, in Teiidae and Gymnophthalmidae it is the XY sex chromosome system which determines the sex of a specimen. On the other hand, in Amphisbaenia and Lacertidae it is the ZW system of sex determination. Sometimes the X1X1X2X2:X1X2Y or Z1Z1Z2Z2:Z1Z2W sex chromosome system occurs in Teiidae / Gymnophthalmidae or in Lacertidae, respectively. The phylogenetical analysis was performed in Mesquite programme and the output from the analysis is a phylogenetical tree with two characters (number of chromosomes and the sex determination system) and the maximum parsimony counted for each of them. Then the interpretation was on the order and it is the main...
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