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Ovarian stimulation and related hyperstimulation syndrome
Pohlová, Nikola ; Komrsková, Kateřina (advisor) ; Ješeta, Michal (referee)
Ovarian stimulation is a key process of assisted reproduction and it helps women in the treatment of infertility. Hormonal ovarian stimulation ensures the growth and development of ovarian follicles. The selected dominant follicle releases an oocyte during ovulation, which is destined for fertilization. In methods of artificial fertilization, multiple follicles are stimulated, and more oocytes are retrieved. The retrieved oocytes are then fertilised in vitro. In this thesis, the benefits and risks of the commonly used stimulation protocols are discussed. The focus is on optimizing stimulation for individual patients, considering their health status, functional ovarian reserve, and individual needs. Special stimulation protocols for patients with polycystic ovary syndrome and for patients with cancer are presented. Ovarian stimulation is also applied to oocyte donors. The most serious risk of ovarian stimulation is ovarian hyperstimulation syndrome. Ovarian stimulation is also used in the rescue of endangered mammalian species or in the breeding of domesticated animals. The study of ovarian stimulation is important for the improvement of reproductive methods in human and veterinary medicine. Key words: follicles, ovarian stimulation, assisted reproduction, ovulation, risks
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Charakterizace oocytů afrických rypošů
KOPECKÁ, Eva
This study is focused on characterisation of oocytes of long-lived rodents, naked mole-rat and giant mole-rat, that are emerging models of long-term oocyte and ovarian quality. In the second part, the effect of repeated ovulations on aging-associated quality decline of oocyte is studied, comparing mouse and giant mole-rat.
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Úspěšnost inseminace klisen ve vztahu k době ovulace
VACEK, David
Mare rate of conception is influenced by many factors. Apart from the impact of the mare and the stallion, the breeder also represents one of these factors. The aim of this master's thesis is to assess the impact of the insemination dose storage, the period between the insemination and ovulation and the mare's age on the actual conception. The results are based on the assessment of forty-nine Czech Warmblood mares from the farms of Újezd and Zduchovice. Their conception rate was monitored throughout the years 2019 and the assessed affecting factors include the kind of semen storage (frozen or fresh chilled semen), the mare's age and the period between ovulation and insemination. The initial assumption that the sort of semen storage (p-value > 0.05) influences the rate of conception has been disproven because the conception rate for frozen semen was 52 % and it increased only by 6 %, amounting to 58 % in total for fresh chilled semen. As far as the age factor is concerned, there was almost no difference (p-value > 0.05) in the ages of gravid and non-gravid mares that were supposed to conceive during their first rut. The average age of pregnant mares is 10 (n = 19), therefore only one year more in comparison with non-pregnant mares (9 year, n = 30). The influence of the period between the insemination and the ovulation on the conception rate has not been proven either. While being monitored, no period between the insemination and ovulation exceeded the recommended length. In conclusion, the breeder can lessen the impact of harmful factors on the conception rate, for example the mare's age, the period between insemination and ovulation and the kind of semen storage.
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Effect of menstrual cycle on performance of women in sport
Růžičková, Kristýna ; Vojtíková, Lenka (advisor) ; Panská, Šárka (referee)
The diploma thesis discusses the possibilities of negative and positive influences on women's menstrual cycle concerning women's performance in sports. The aim of the thesis is to help spread awareness about the mentioned issue and also inspire coaches to be more cautious about this topic while training women. The theoretical part describes the physiological processes of a women's menstrual cycle, hormonal system, and the impact of the menstrual cycle's phases on women's performance in sports. The thesis further analyses the difference between the two menstrual cycle phases in particular endurance performance. The results were completed by adding a poll that focused on personal feelings during testing and in general, the impact of the menstrual cycle regarding sports activities.
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Effects of menstrual cycle on sports performance
Hysková, Dominika ; Hráský, Pavel (advisor) ; Kinkorová, Ivana (referee)
Title: Effects of menstrual cycle on sports performance Objectives: The goal of this thesis is to detect possible effect of current menstrual phase on womens sports training. It aims to contribute to possible individual approach to the training and suggest the options on how to adjust it based on a current menstrual phase of the charge. This paper is a pilot project for my master's thesis. Methods: Search of monograph which pursue issue of menstrual cycle. Results: Menstrual cycle does not affect sports training significantly, however minor effects were identified. It is mainly development of power abilities during the follicular phase. Compared to the luteal phase, the power performance was observed to be improved. Another worth mentioning finding is increased injury occurence during the follicular phase of menstrual cycle. Keywords: menstrual phases, hormons, pathology of the menstrual cycle, physical performance
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Využití přípravků umožňujících kontrolované uvolňování hormonální látky v umělé reprodukci ryb
PECHA, Oldřich
This bachelor thesis was focused on identification of species-optimized preparations based on PLGA microparticles in terms of length and intensity of release and composition of active substance (GnRHa, metoclopramide), which would be used in artificial reproduction of selected fish species - pike (Esox lucius). Under the conditions of the Experimental Fish Farm, FROV JČU in Vodňany and the Tábor Štičí líheň ESOX s.r.o. the first two-week experiment was conducted to achieve the desired results, which were achieved with males of pike. A year later, the experiment took place again in Tabor and newly in the hatchery in Mydlovary. This time we focused on females of pike. During the first research, we have achieved satisfactory results and confirmed that we are going in the right direction. Combination of GnRH with dopamine antagonist Metoclopramide has been shown to be most effective. The best results were achieved by application of 20?g GnRHa.kg-1 + 20mg MET.kg-1, where we achieved positive results in all parameters - increased milt production volume, increased sperm motility and speed, decreased 11-ketotestosterone concentration. During the second experiment with female, we showed that the combination of GnRH + MET at a concentration of 20?g GnRHa.kg-1 + 20mg MET.kg-1 has a positive effect on ovulation achievement, latency time reduction and higher concentration of 40?g GnRHa.kg-1 + 20 mg MET.kg-1 also for fertilization and hatching of eggs, but at the cost of lower fecundity. The results of the experiments show that by using this combination and the correct concentration of GnRH with the dopamine antagonist it is possible to induce maturation of the gametes of male as well as female of pike fish. However, there is still room for improvement of female.
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Reproduction as an intensifying factor in the breed of pigs
PROVAZNÍKOVÁ, Alena
The aim of this work was to discover weak points and their impacts on breeding of pigs. It consequently suggests a successful management of company in the field of reproduction and nutrition of pigs. The work is focused on basic physiological and technological assumptions about reproduction of pigs and it deals with the oestrus, ovulation, classifying gilts for breeding, the onset of estrus after weaning piglets, the usage of natural methods and controlled reproduction, the usage of wet nurses sows systems, artificial insemination and current physiology of the reproductive cycle of the sow. A separate chapter of the work is focused on the nutrition and feeding of individual categories of pigs intended for reproduction (breeding boars, gilts, dimpling, pregnant and lactating sows) because nutrition has a huge impact on the breeding. Another chapter discusses about technological conditions and it deals with the diet of pigs as well as with the stable environment which is mentioned mainly from a technological point of view. The final part of the work is devoted with the development of reproduction during the last period and it concludes recommendations in the practical usage, mainly in the field of reproduction and rearing piglets.
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