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Phenotypic and functional characterization of leukocytes in patients with immune system dysregulation
Fejtková, Martina ; Kanderová, Veronika (advisor) ; Vlková, Marcela (referee) ; Balounová, Jana (referee)
Inborn errors of immunity (IEI) are a heterogenic group of diseases of the immune system causing dysregulations of both innate and adaptive immunity. New altered immune-related genes are discovered every year, nowadays reaching over 400. (Tangye et al., 2020) Here three new autoinflammatory disorders of IEI patients are described. Toll-like receptors (TLR) 7 and 8 are endosomal receptors in the innate immune response against external pathogens and endogenous autoantigens. A dysregulation in TLR7 and TLR8 in mice causes autoimmunity and inflammation, however, in humans, the immunopathology of TLR8 and TLR7 remains unclear. We identified a novel X-linked c.1715G>T mutation in TLR8 that leads to autoimmune haemolytic anaemia and autoinflammation in male twins caused by dysregulation in TLR8 and TLR7 response especially in myeloid cells (low TLR8 protein expression, cross-reactivity of TLR8 for TLR7 ligands and enhanced TLR7 response). Hematopoietic cell kinase (HCK) belongs to the Src family of kinases and is involved in myeloid cell migration, adhesion and degranulation. Kinase activity in the immune response must be strictly regulated. In HCK, this regulation is based on C-terminal inhibitory tyrosine, which when phosphorylated, HCK kinase activity is switched off. We identified a heterozygous...
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Role of surface defects in ceria-based catalysis
Tovt, Andrii ; Mysliveček, Josef (advisor) ; Čechal, Jan (referee) ; Švec, Martin (referee)
Title: Role of surface defects in ceria-based catalysis Author: Andrii Tovt Department: Department of Surface and Plasma Science Supervisor of the doctoral thesis: doc. Mgr. Josef Mysliveček Ph.D., Department of Surface and Plasma Science Abstract: This work concentrates on the analysis of fundamental physicochemical properties of Pt-CeOx, single-atom Pt1 /CeOx, and inverse CeOx/Cu(111) catalysts. Preparation method for stabilized atomically-dispersed Pt2+ ions on ceria was developed and adsorption sites for Pt ions were thoroughly studied using advanced surface science techniques supported by theoretical methods. The mechanism of Pt2+ stabilization on ceria steps was revealed and the step capacity towards Pt2+ ions was estimated. Also, the preparation method for well-ordered cerium oxide ultrathin films with different stoichiometry and ordering of surface oxygen vacancies was developed, and the Ceria/Cu(111) interaction was investigated. Key words: heterogeneous catalysis, model systems, single-atom catalysis, platinum ions, cerium oxide.
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Role of surface defects in ceria-based catalysis
Tovt, Andrii ; Mysliveček, Josef (advisor)
Title: Role of surface defects in ceria-based catalysis Author: Andrii Tovt Department: Department of Surface and Plasma Science Supervisor of the doctoral thesis: doc. Mgr. Josef Mysliveček Ph.D., Department of Surface and Plasma Science Abstract: This work concentrates on the analysis of fundamental physicochemical properties of Pt-CeOx, single-atom Pt1 /CeOx, and inverse CeOx/Cu(111) catalysts. Preparation method for stabilized atomically-dispersed Pt2+ ions on ceria was developed and adsorption sites for Pt ions were thoroughly studied using advanced surface science techniques supported by theoretical methods. The mechanism of Pt2+ stabilization on ceria steps was revealed and the step capacity towards Pt2+ ions was estimated. Also, the preparation method for well-ordered cerium oxide ultrathin films with different stoichiometry and ordering of surface oxygen vacancies was developed, and the Ceria/Cu(111) interaction was investigated. Key words: heterogeneous catalysis, model systems, single-atom catalysis, platinum ions, cerium oxide.
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Model Systems in the Study and Teaching of Molecular Biology
Kripnerová, Michaela ; Ehler, Edvard (advisor) ; Němečková, Linda (referee)
Molecular biology and genetics are currently very fast developing disciplines. One of the possibilities how to increase the pupils' interest in studying of molecular biology and genetics, and to support their own curiosity is to use research-based teaching strategies. Although Czech Education tries to correspond with this progress, teaching of molecular biology and genetics is limited not only by financial and material limits but also by a lack of vocational education. The diploma thesis is divided into theoretical and practical part. The theoretical part deals with the position of molecular biology in teaching and model systems in the study and teaching of molecular biology and genetics. The practical part is divided into three chapters. The goal of the first chapter was to present available teaching materials suitable for a teaching at grammar schools, especially with regard to the availability of manuals for practical seminars. The second chapter summarized the results of a questionnaire survey among grammar school teachers, which concerns the position of molecular biology and genetics in teaching. Both of the above-mentioned goals have shown that more needs to be done about the molecular biology of genetics. According to the teachers, students who would like to continue to pursue this...
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Role of surface defects in ceria-based catalysis
Tovt, Andrii ; Mysliveček, Josef (advisor)
Title: Role of surface defects in ceria-based catalysis Author: Andrii Tovt Department: Department of Surface and Plasma Science Supervisor of the doctoral thesis: doc. Mgr. Josef Mysliveček Ph.D., Department of Surface and Plasma Science Abstract: This work concentrates on the analysis of fundamental physicochemical properties of Pt-CeOx, single-atom Pt1 /CeOx, and inverse CeOx/Cu(111) catalysts. Preparation method for stabilized atomically-dispersed Pt2+ ions on ceria was developed and adsorption sites for Pt ions were thoroughly studied using advanced surface science techniques supported by theoretical methods. The mechanism of Pt2+ stabilization on ceria steps was revealed and the step capacity towards Pt2+ ions was estimated. Also, the preparation method for well-ordered cerium oxide ultrathin films with different stoichiometry and ordering of surface oxygen vacancies was developed, and the Ceria/Cu(111) interaction was investigated. Key words: heterogeneous catalysis, model systems, single-atom catalysis, platinum ions, cerium oxide.
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Role of surface defects in ceria-based catalysis
Tovt, Andrii ; Mysliveček, Josef (advisor) ; Čechal, Jan (referee) ; Švec, Martin (referee)
Title: Role of surface defects in ceria-based catalysis Author: Andrii Tovt Department: Department of Surface and Plasma Science Supervisor of the doctoral thesis: doc. Mgr. Josef Mysliveček Ph.D., Department of Surface and Plasma Science Abstract: This work concentrates on the analysis of fundamental physicochemical properties of Pt-CeOx, single-atom Pt1 /CeOx, and inverse CeOx/Cu(111) catalysts. Preparation method for stabilized atomically-dispersed Pt2+ ions on ceria was developed and adsorption sites for Pt ions were thoroughly studied using advanced surface science techniques supported by theoretical methods. The mechanism of Pt2+ stabilization on ceria steps was revealed and the step capacity towards Pt2+ ions was estimated. Also, the preparation method for well-ordered cerium oxide ultrathin films with different stoichiometry and ordering of surface oxygen vacancies was developed, and the Ceria/Cu(111) interaction was investigated. Key words: heterogeneous catalysis, model systems, single-atom catalysis, platinum ions, cerium oxide.
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