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Testing of Surge Arresters
Jeřábek, Lukáš ; Paar, Martin (referee) ; Krbal, Michal (advisor)
This bachelor's thesis is devoted to metal oxide surge arresters. After historical and theoretical introduction, which explains their principles and describes their characteristical properties, analysis of the standard IEC 60099-4 and testing according to the standard with focus on the type tests follows. This chapter is also amended with review of the standard's imperfections and with its most probable future development. The work concludes with a market survey, a review of the largest manufacturers and a comparison of their products.
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Revealing phosphoproteins playing role in tobacco pollen activated in vitro
Fíla, Jan ; Honys, David (advisor) ; Fischer, Lukáš (referee)
5 Abstract Tobacco mature pollen rehydrates in vivo on a stigma tissue, and develops into the rapidly-growing pollen tube. This rehydration process is accompanied by the de-repression of stored mRNA transcripts, resulting in the synthesis of novel proteins. Furthermore, such metabolic switch is also likely to be regulated on the level of post-translational modifications of the already-present proteins, namely via phosphorylation, since it was shown to play a significant regulatory role in numerous cellular processes. Since only a minor part of proteins is phosphorylated in a cell at a time, the employment of various enrichment techniques is usually of key importance. In this diploma project, metal oxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix was applied in order to enrich phosphoproteins from the mature pollen and the 30-minute in vitro activated pollen crude protein extracts. The enriched fraction was separated by both 2D-GE and gel-free liquid chromatography (LC) approaches with subsequent mass spectrometric analyses. Collectively, 139 phosphoprotein candidates were identified. Additionally, to broaden the number of phosphorylation sites identified, titanium dioxide phosphopeptide enrichment of trypsin-digested mature pollen crude extract was performed. Thanks to the...
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Preparation of Nanocomposites of Metal Oxides in Plasma Polymer and Study of Their Properties
Polonskyi, Oleksandr ; Biederman, Hynek (advisor) ; Novák, Stanislav (referee) ; Kadlec, Stanislav (referee)
Title: Preparation of Nanocomposites of Metal Oxides in Plasma Polymer and Study of Their Properties Author: Oleksandr Polonskyi Department: Department of Macromolecular Physics, MFF UK Supervisor of the doctoral thesis: Prof. RNDr. Hynek Biederman, DrSc. Department of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University in Prague Abstract: This thesis is devoted to a study of nanocomposite films Al (Al oxide)/plasma polymer prepared by various techniques using magnetron sputtering, plasma polymerization and cluster beam deposition. The formation and deposition of metal/metal oxide nanoclusters using a gas aggregation cluster source (GAS) was also considered. The role of low concentration of oxygen in the aggregation gas on the process of Al and Ti cluster deposition was studied. Properties of the nanoclusters and nanocomposite films were characterized by various techniques. Morphology of the nanocomposites was examined by AFM, TEM or HRTEM and SEM. Elemental analysis and chemical composition of the films were studied by XPS and FTIR. Optical characterization of the prepared films was done by UV-Vis spectroscopy and spectroscopic ellipsometry. It has been shown that using GAS nanocomposite Al(AlxOy)/C:H may be prepared. Keywords: nanocomposite thin film, plasma polymer, metal...
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Revealing phosphoproteins playing role in tobacco pollen activated in vitro
Fíla, Jan ; Honys, David (advisor) ; Fischer, Lukáš (referee)
5 Abstract Tobacco mature pollen rehydrates in vivo on a stigma tissue, and develops into the rapidly-growing pollen tube. This rehydration process is accompanied by the de-repression of stored mRNA transcripts, resulting in the synthesis of novel proteins. Furthermore, such metabolic switch is also likely to be regulated on the level of post-translational modifications of the already-present proteins, namely via phosphorylation, since it was shown to play a significant regulatory role in numerous cellular processes. Since only a minor part of proteins is phosphorylated in a cell at a time, the employment of various enrichment techniques is usually of key importance. In this diploma project, metal oxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix was applied in order to enrich phosphoproteins from the mature pollen and the 30-minute in vitro activated pollen crude protein extracts. The enriched fraction was separated by both 2D-GE and gel-free liquid chromatography (LC) approaches with subsequent mass spectrometric analyses. Collectively, 139 phosphoprotein candidates were identified. Additionally, to broaden the number of phosphorylation sites identified, titanium dioxide phosphopeptide enrichment of trypsin-digested mature pollen crude extract was performed. Thanks to the...
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Testing of Surge Arresters
Jeřábek, Lukáš ; Paar, Martin (referee) ; Krbal, Michal (advisor)
This bachelor's thesis is devoted to metal oxide surge arresters. After historical and theoretical introduction, which explains their principles and describes their characteristical properties, analysis of the standard IEC 60099-4 and testing according to the standard with focus on the type tests follows. This chapter is also amended with review of the standard's imperfections and with its most probable future development. The work concludes with a market survey, a review of the largest manufacturers and a comparison of their products.
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Thin Photoresponding Elements with Frequency and Amplitude Modulations
Klusoň, Petr ; Morozová, Magdalena ; Dzik, P. ; Veselý, M.
Nanoscale templated metal oxide thin films undoubtedly appertain to the most extensively studied materials due to their optical and electrochemical properties. Their photo-induced properties arise from the semiconductor nature, especially from the ability of the light quantum absorption followed by the charge carrier generation. In order to produce metal oxide thin layer layers, the liquid sol containing also the templating bodies, and confining nanoparticles, must be coated onto a substrate. Various coating techniques have been used for this purpose, such as dip-, spin- or spraycoating. Recently a new promising deposition technique has appeared. The novel approach is usually termed piezoelectric jet material deposition or shortly material printing. In this case the ink is the specially formulated liquid purposed for transporting functional components onto the substrate surface. This technique brings the possibility of direct patterning, i.e. the fabrication of 2D patterns on the substrate without the need of any mechanical or optical masking. Material printing has been already employed for the deposition of variety functional materials.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22013102415160 - PDF
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Super/Subcritical Fluid Extractions for Synthesis of Porous Metal Oxides
Matějová, Lenka ; Matěj, Z. ; Cajthaml, Tomáš ; Šolcová, Olga
In this study the influence of individual post-treatment steps on the crystallinity, purity and textural properties of prepared metal oxides - TiO2, ZrO2, TiO2-ZrO2 etc. in powdery form (aerogels) – has been studied. Titania and zirconia organogels were prepared as reaction semi-products of the sol-gel process controlled in reverse micellar solution of nonionic surfactant Triton X-114. in combination with a) subcritical fluid extractions (using water and subsequently methanol like pressurized solvents, SubWE/PFE) and b) calcination. The effect of various extraction conditions on textural and structural properties of metal oxides has been investigated. Besides that the reproducibility of these material properties has been also observed.
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