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Influence of Storage Conditions on Metabolic Profile of Apples
Duroňová, Kateřina ; Vávrová, Milada (referee) ; Kráčmar, Stanislav (referee) ; Márová, Ivana (advisor)
The goal of presented dissertation has been complex analysis of changes in content of fatty acids, enzymatic and low-molecular antioxidants in apples and related evaluation of perception of originators of storage diseases for apples stored in various conditions. Main part of the work has been dedicated to study of the impact of storage of apples in modified atmosphere with reduced amount of oxygen and in reference “normal” atmosphere for six months. Next part of the work has been dedicated to study how apple storage in common, consumer affordable, conditions (storage in a cellar, in a refrigerator, and in room temperature) affects content of low-molecular antioxidants. For testing has been selected apple kinds Jonagored, Golden Delicious, Idared, Šampion, and Granny Smith. Within the scope of this work has been optimized the method for determination of fatty acids in plant material with higher content of wax. The measured values imply the apples are valuable source of many important nutrition substances like vitamins, provitamins and antioxidants. During the storage process these substances exhibit considerable protective function. Long-term storage, mainly in the atmosphere with reduced amount of oxygen (FAN), enables preservation of majority of these important nutrition substances depending on the kind of apple and conditions of storage. Freezing process is conservative to apples (mainly in the presence of protective substances), while in the process of drying the values of all monitored antioxidants decrease depending on temperature and conditions of drying. Upon the choice of the storage method one must consider nutrition, sensoric characteristic and consumer demands.
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Effect of addition of microbial or vegetable oil on the efficiency of cosmetic products
Kovářová, Karolína ; Hoová, Julie (referee) ; Němcová, Andrea (advisor)
This thesis focuses on the influence of the fatty acid profile and the concentration of active substances on the abilities to hydrate and to absorb into the skin of cosmetic emulsions. Coconut and macadamia oil, which are common cosmetic ingredients, were used as vegetable active ingredients. The last active ingredient used was the microbial oil, which was prepared as part of this work. Four strains of oleaginous yeast Metschnikowia were tested for the overproduction of microbial lipids in various culture media. Based on the results of gas chromatography, the yeast strain Metschnikowia pulcherrima (149) was selected. The chosen culture medium contained apple cider as a carbon source and had a 10 % sodium chloride content, inducing osmotic stress. Cosmetic emulsions with different concentrations of active ingredients were prepared. These emulsions were tested by sensory analysis. After application, the skin was analyzed with a skin camera that measured desquamation and sebum. The degree of desquamation is directly related to skin hydration and the level of sebum reflects the ability of the emulsion to be absorbed into the skin. It was found that if the user is not used to applying hydrating cosmetic emulsions regularly, but only solves the immediate problem of dry skin, it is advantageous to use an emulsion with an active ingredient content of 5.0 % or more. However, commercially available hydrating creams typically contain effective vegetable oils in lower concentrations. Furthermore, it was found that microbial oil could be a very interesting cosmetic ingredient.
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Lipid composition of biological membranes and life-histories in songbirds
Šimonová, Kateřina ; Tomášek, Oldřich (advisor) ; Sam, Kateřina (referee)
Differences in the composition of cell membranes are considered to be the physiological mechanism responsible for different metabolic rates, life span and consequently, different life-histories. The membrane pacemaker theory suggests that higher membrane unsaturation may be an important factor responsible for higher metabolic rate and shorter life span of organisms. The alternative hypothesis, on the other hand, assumes that membranes can function as structural antioxidants, and thus that higher membrane unsaturation may be associated with longer life span. The aim of this work was to test how changes in the fatty acid composition of membranes depends on body size, latitude and altitude, within which different life-histories and metabolic rate were observed. Temperate and tropical lowland songbird species were chosen for the models testing the effect of latitude, and only tropical species of songbirds were chosen for the models testing the effect of altitude. Part of the work was molecular sex determination. Using gas chromatography and mass spectrometry, the amount of individual fatty acids contained in red blood cell phospholipids was obtained from blood samples. The results showed that the composition of the membranes in most cases correlates with body size, which also correlates with almost all...
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Lipid composition of biological membranes and life-histories in songbirds
Šimonová, Kateřina ; Tomášek, Oldřich (advisor) ; Sam, Kateřina (referee)
Differences in the composition of cell membranes are considered to be the physiological mechanism responsible for different metabolic rates, life span and consequently, different life-histories. The membrane pacemaker theory suggests that higher membrane unsaturation may be an important factor responsible for higher metabolic rate and shorter life span of organisms. The alternative hypothesis, on the other hand, assumes that membranes can function as structural antioxidants, and thus that higher membrane unsaturation may be associated with longer life span. The aim of this work was to test how changes in the fatty acid composition of membranes depends on body size, latitude and altitude, within which different life-histories and metabolic rate were observed. Temperate and tropical lowland songbird species were chosen for the models testing the effect of latitude, and only tropical species of songbirds were chosen for the models testing the effect of altitude. Part of the work was molecular sex determination. Using gas chromatography and mass spectrometry, the amount of individual fatty acids contained in red blood cell phospholipids was obtained from blood samples. The results showed that the composition of the membranes in most cases correlates with body size, which also correlates with almost all...
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Influence of Storage Conditions on Metabolic Profile of Apples
Duroňová, Kateřina ; Vávrová, Milada (referee) ; Kráčmar, Stanislav (referee) ; Márová, Ivana (advisor)
The goal of presented dissertation has been complex analysis of changes in content of fatty acids, enzymatic and low-molecular antioxidants in apples and related evaluation of perception of originators of storage diseases for apples stored in various conditions. Main part of the work has been dedicated to study of the impact of storage of apples in modified atmosphere with reduced amount of oxygen and in reference “normal” atmosphere for six months. Next part of the work has been dedicated to study how apple storage in common, consumer affordable, conditions (storage in a cellar, in a refrigerator, and in room temperature) affects content of low-molecular antioxidants. For testing has been selected apple kinds Jonagored, Golden Delicious, Idared, Šampion, and Granny Smith. Within the scope of this work has been optimized the method for determination of fatty acids in plant material with higher content of wax. The measured values imply the apples are valuable source of many important nutrition substances like vitamins, provitamins and antioxidants. During the storage process these substances exhibit considerable protective function. Long-term storage, mainly in the atmosphere with reduced amount of oxygen (FAN), enables preservation of majority of these important nutrition substances depending on the kind of apple and conditions of storage. Freezing process is conservative to apples (mainly in the presence of protective substances), while in the process of drying the values of all monitored antioxidants decrease depending on temperature and conditions of drying. Upon the choice of the storage method one must consider nutrition, sensoric characteristic and consumer demands.
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Effect of alternative feed on content of omega 3 fatty acids in fish muscles
KREJSA, Jakub
The aim of my bachelor thesis was to evaluate the effect of fish oil substitution for rapeseed oil in the feed on the fat content and fatty acid composition in the flesh of Brook trout (Salvelinus fontinalis) in the finishing feeding technology. The experiment was conducted in 2012 for the period of 135 days. The individuals of Brook trout were divided into four groups. The first two groups were fed with the feed containing only rapeseed or fish oil. The feeding of the remaining two groups was divided into two phases. In the first phase the fish oil was partly replaced by the rapeseed oil. In the second phase the fish oil was replaced by the feed which contained fish oil only. It was proved that a half replacement of the fish oil with the rapeseed oil does not influence the content of fat in the flesh of Brook trout. We observed that the fish had the higher content of MUFA and n-6 PUFA and lower content of n-3 PUFA, DHA, EPA and n-3/n-6 with increasing period where the feed with rapeseed oil was served. Following application of the finishing feeding technology with the use of feed with fish oil had the opposite effects. Furthermore, the dilution model was proved to be highly reliable for the prediction of the fatty acids composition. Based on these findings, it is possible to predict changes of fatty acid composition in the flesh of Brook trout as well as to predict how long is needed to apply finishing feeding to reach required content of EPA+DHA or n-3 HUFA.
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