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Changes of motor performance after epileptic seizure in developing laboratory rats
Hanáková, Helena ; Mareš, Pavel (advisor) ; Stuchlík, Aleš (referee)
The aim of the work was analysis of postictal period - possible changes of motor performance after seizure. Epileptic afterdischarges are induced by stimulation cof ortical sensorimotor area in rat. We will use the intensity to produce human myoclonic seizure in 12-, 18- a 25- days old animals. The youngets and the oldest groups differ by the absence (12-day-old rats) or presence (25-day-old- ones) of postictal refractoriness. Control groups will be formed by intact animals. Individual groups will be observed immidiatelly after seizure and after different intervals. Keywords: epileptic afterdischarge, postictal period, motor performance, laboratory rat, development
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The influence of perinatal hypoxia on motoric development on laboratory rat and means of therapy
Vachovcová, Sylva ; Mareš, Pavel (advisor) ; Stuchlík, Aleš (referee)
Severe perinatal hypoxia represents a substantial brain injury in human newborns. This Diploma thesis is focused on long-term motor outcome of laboratory rat after moderate perinatal hypoxia. We described some behavioral test for detection motor development and presented the influence of perinatal hypoxia on central nervous system. We also discussed an effect of agonists and antagonists of adenosine A1 receptor in brain. The aim of an experimental part was an evaluation of long-term motor behavior in rats affected by perinatal hypoxia. To cause perinatal hypoxia we put pregnant female rats to a hypoxic (10% O2) normobaric room in 11th day of their gestation. The pregnant female rats stayed in hypoxic room until they gave a birth and 6 more days after birth with their litters. For classification of motor development we used battery of tests of motor coordination. These tests correspond to the level of development of the rat. Then a group of rats with perinatal hypoxia was treated by a single administration of an agonist of adenosine A1 receptor 2-chloro-N(6)- cyclopentyladenosin (CCPA) in postnatal day 14. The animals affected by perinatal hypoxia show motor deficits in 3 from 4 selected behavioral tests. Otherwise, this motor behavior was no longer detected in young adults. The rats affected by...
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The effect of new synthetized drugs on electrical activity of rat isolated heart
Korčáková, Ivona ; Janoušek, Oto (referee) ; Ronzhina, Marina (advisor)
This thesis deals with the influence of the newly synthesized drug on the electrical activity of the isolated heart of rat. Part of the thesis is a theoretical analysis of the use laboratory animals in experiments and ethical aspects related to the use of laboratory animals. There is also an analysis of drug testing, test substances, electrocardiography and methods used to detect and measure ECG signal. The two algorithms used for the QT interval are automated. The QT interval is the main indicator of cardiotoxicity and is considered to be the gold standard in evaluating the effect of the drug. In the practical part the ECG records obtained at the Faculty of Medicine at Masaryk University in Brno are processed. These records are dimmed manually and automatically. The manual dimming was consulted with a specialist in cardiography and statistically processed. Statistical processing served to compare with the results of the automatic ECG measurement. The algorithm is used to automate the measurement, and the results are compared with the reference points obtained from cardiology experts and manual measurement results. This work serves as a pilot study for the development and testing of a new active substance.
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The affect of perinatal hypoxia on locomotor development of laboratory rat
Vachovcová, Sylva ; Mareš, Pavel (advisor) ; Slabý, Kryštof (referee)
Perinatální hypoxie je závažným problémem u předčasně narozených dětí i u dětí narozených v termínu. Představuje vysoké riziko behaviorálních a neurologických poruch, a přestože se současná neonatální intenzivní péče stále zdokonaluje, rozsáhlá mozková léze v důsledku perinatálního inzultu obnáší výrazný klinický problém. Studie na zvířatech jsou proto stále nezbytné. Model perinatální hypoxie u laboratorních potkanů tak představuje výzkumné pole, kde se popisuje řada neurologických defektů, jako jsou poruchy lokomočních dovedností a deficity kognitivních funkcí u nedospělých i dospělých perinatální hypoxií postižených zvířat. Jejich mozková tkáň může sloužit k histologickému rozboru pro určení rozsahu mozkové léze a mnohdy jsou u těchto zvířat zaznamenány růstové poruchy, které jsou často spatřovány u dětské mozkové obrny.
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Se-Metabolism inside the mammalian organism fed Se-supplemented Brassica napus forage
Žíla, Ondřej ; Čadková, Zuzana (advisor) ; Václav, Václav (referee)
The aim of the thesis was to determine whether the individual Se-speciation in the mammalian organism are affected by the form of received selenium. Selenium is an essential micronutrient important for humans and animals. It plays an important role in the antioxidant protection of the organism and in the conversion of thyroid hormones. In our experiment the laboratory Wistar rats were divided into three groups. Each group had a different diet. The rats were fed with selenium in the form of soy, sodium selenite and extracted rapeseed meal. Urine samples were regularly collected during the four-week experiment and in the end of the feeding study, the blood serum was also collected. The total selenium content was measured by ICP-MS, while the individual Se-speciation in urine and serum by HPLC coupled with ICP-MS. In the urine the identified speciation were methylselenocystein (MeSeCys), trimethylselenium (TMSe) and selenosugar 1 and 3. In the blood serum the measured speciation were TMSe, selenite, selenate and selenosugar 1. For the group fed with sodium selenite the measured values in the urine were generally higher, this might be due to a higher overall intake and also an inorganic form of selenium with a lower absorbency. Groups that received selenium from plant sources took in several Se-compounds and the total measured content of Se-speciation and secretion dynamics were not significantly different. Additionally speciation of selenosugar 2 was measured for the group fed with rapeseed meals, which in the other groups did not appear. When receiving selenium from plant sources the biotransformation in the mammalian organism differs in comparison to receiving selenium from mineral salts. The initial hypothesis that Se-speciation is influenced by the form of selenium administered in the diet was confirm by our results. Since the group fed rapeseed showed similar results as the group fed a standard feed with soy, the extracted rapeseed meal could serve as a good source in livestock nutrition.
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