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Monitoring kyseliny askorbové v ovocných nápojích
Chytilová, Barbora
The thesis deals with monitoring of ascorbic acid and other substances in selected fruit drinks. The theoretical part begins with a summary of knowledge about the occurrence and importance of ascorbic acid and their physiological effects. Furthermore, the content of vitamin C in individual fruits is compared. The following part deals with the division of soft drinks and raw materials, used for the production of soft fruit drinks. Part of the thesis is focused on the technology of fruit juice production obtained by direct pressing of fruit material and technology of fruit drinks production from fruit concentrates. The practical part is focused on determination of vitamin C concentration as well as vitamins of group B (B1, B2, B3, B5, B6) and organic acids (citramic acid, citric acid, malic acid, pyruvic acid and succinic acid) in samples of direct fruit juices and juice from concentrate. Mass spectrometer-coupled high-performance liquid chromatography (HPLC-MS) was used to detect vitamin C and other substances.
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Faktory ovlivňující množství vitaminu C v zeleném koření
Průchová, Simona
The thesis deals with particular green spice like Mentha longifolia L., Mentha spicata L., Mentha tomentosa Sm. 'Fíková', Mentha suaveolens Ehrt. 'Jablečná', Mentha piperita 'Persephone', Origanum vulgare L., Origanum heracleoticum L., Origanum vulgare L.'Aureum', Origanum vulgare L. 'Thumbles variety', Satureja montana L., Satureja hortensis L., Salvia glutinosa L., Salvia verbanaca L., Salvia verticilata L., Salvia lyrata L., Salvia przewalskii Maxim, Salvia officinalis L. 'Purpurascens', Allium nutans L., Allium stipitatum L., Allium tuberosum Rottl. Ex Spreng. In theoretic part a botanical charakteristics of the spice, legislation concerning the green spice, new information about vitamin C and factors influencing the content of ascorbic acid in plants are described. In practical part, the content of vitamin C in vegetative and generative growth phase has been determined. Results have been evalueted and compared.
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Zdravotní aspekty konzumace ovoce a ovocných šťáv
Stašková, Nicole
The literary part of The Bachelor thesis covers the general characteristics of the fruit, its influences on our health and the content of healthy substances (vitamins, antioxidants, minerals). The practical part of this bachelor thesis deals with the analysis of fruit (apple, pear, pineapple, orange, lemon, grapefruit) and fruit juices (100% orange juice from concentrate, 100% cold pressed orange juice, multivitamin fruit juice, Bio juice from black currant (unfiltered), 100% cold pressed pineapple juice, 100% juice of pink grapefruit, Bio pear juice, Bio apple juice from concentrate). Content of the ascorbic acid was analyzed for all samples by the high-performance liquid chromatography method. The results were evaluated and compiled into tables and graphs and were compared with foreign authors.
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Alternativy k SO2
Měřínská, Iva
The bachelor thesis describes the issue of sulfur dioxide and substances proposed for its replacement. The theoretical part defines the basic problem areas - the usage of sulfur dioxide in wine-making, forms of sulfur dioxide in wine, methods of application, impacts on human health and methods to reduce sulfur dioxide in wine. The practical part is focusing on the experiment which was executed excluding the usage of sulfur dioxide during the vinification. The resulting wines are monitored from the analytical and sensory point of view. The measured results are adequately evaluated at the end of the thesis.
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Study of Boron-Carbohydrate Interaction
Vejvodová, Tereza ; Jelínek, Ivan (advisor) ; Coufal, Pavel (referee)
The interaction between boron and sugars is significant for understanding the role of boron as a carrier for nucleotides and carbohydrates. Arylboronic acids also have wide application as sugars sensors because they are small and flexible molecules. Boron complexes play a key role in boron neutron capture therapy. This radiotherapy is important in the treatment of melanoma, glioblastoma multiforme and other types of cancer. Boron complexes with ascorbic acid and glucose were prepared in this thesis and then characterized by high-resolution mass spectrometry (HRMS). Synthesised complexes were studied using collision-induced dissociation (CID), which led to the clarification of their fragmentation mechanism. Key Words Boron complexes, ascorbic acid, glucose, carbohydrates, mass spectrometry, electrospray ionization, ESI-MS, collision-induced dissociation
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Analytical study of reduction of ammonium perrhenate using selected reduction agents
Lišková, Jana ; Jelínek, Ivan (advisor) ; Dian, Juraj (referee)
This thesis is focused on analysis of reduction of ammonium perrhenate using less frequent reducing agents. Ascorbic acid and acetylhydrazine were chosen as less common reducing agents. Reductions were observed leveraging capillary zone electrophoresis and UV/Vis spectroscopy. Measurements were carried out both in acidic and in basic environments. Reduction of peak area of ammonium perrhenate was observed using capillary zone electrophoresis after addition of reducing agent. Rate of absorption of ammonium perrhenate was monitored with UV/Vis spectroscopy. Furthermore, the possibility of shifting the equilibrium of the reaction to the benefit of the reduction products was monitored by the addition of the complexing agent. Py- rogallol (benzene-1,2,3-triol) was used as the ligand and acetylhydrazine was selected as the reducing agent. The reduction was monitored by capillary zone electrophoresis. The identity of the resulting complex was confirmed with ESI/MS. Keywords rhenium, rhenium complexes, ascorbic acid, acetylhydrazine, capillary zone electrophore- sis, UV/Vis spectroscopy, mass spectrometry
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Sledování kvalitativního a kvantitativního zastoupení vitaminů v masozeleninových přesnídávkách určených pro dětskou vyživu
Solčány, Veronika
This thesis deals with the qualitative and quantitative representation of vitamins in the meat-vegetables based infant food. The work is divided into the theoretical and the experimental part. The theoretical part describes a child nutrition and the vitamins. In the chapters, dealing with vitamins, is a description of particular vitamins, their biological functions in the body, the recommended daily dose and their stability during food processing. These chapters concern fat-soluble vitamins (vitamin A, D, E and K) and water-soluble vitamins (B-group vitamins and vitamin C) as well. The work deals with nutrition of infants, toddlers and young children under age of 10 years too. For the each age group there is given the daily recommended dose of vitamins. Experimental part deals with the quantification of vitamins (retinol, ascorbic acid, A-tocopherol and B-carotene) by the HPLC analysis in the samples of the meat-vegetables based infant food. The influence of storage time and conditions (exposure to daylight vs storage in the dark) was measured on concetration of the examined vitamins in the samples. The results were statistically analyzed using software Statistica 12 (Wilcoxon signed-rank test, linear regression, p<0,05) and showed, that all monitored vitamins degrades during storage. The concetration of vitamins in the product is also influenced by the storage conditions (the faster degradation of vitamins occurred in samples exposed to daylight).
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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Význam glutathionu pro kvalitu hroznů a vína
Kandourova, Alexandra
Glutathione is an important constituent of grape, must, and wine. Special emphasis is given to its occurrence in grapes, must, and wine; its role as an antioxidant in wine. The effect of glutathione on aroma compounds is also outlined. There are information on the positive effects of the use of glutathione-enriched preparations in wine industry. It is evaluated its effectiveness in combination with ascorbic acid against oxidative color formation.
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